Atomistry » Magnesium » PDB 2reu-2uxc » 2uag
Atomistry »
  Magnesium »
    PDB 2reu-2uxc »
      2uag »

Magnesium in PDB 2uag: Udp-N-Acetylmuramoyl-L-Alanine:D-Glutamate Ligase

Enzymatic activity of Udp-N-Acetylmuramoyl-L-Alanine:D-Glutamate Ligase

All present enzymatic activity of Udp-N-Acetylmuramoyl-L-Alanine:D-Glutamate Ligase:
6.3.2.9;

Protein crystallography data

The structure of Udp-N-Acetylmuramoyl-L-Alanine:D-Glutamate Ligase, PDB code: 2uag was solved by J.Bertrand, E.Fanchon, O.Dideberg, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 8.00 / 1.70
Space group P 41
Cell size a, b, c (Å), α, β, γ (°) 65.560, 65.560, 136.010, 90.00, 90.00, 90.00
R / Rfree (%) 18 / 20.6

Magnesium Binding Sites:

The binding sites of Magnesium atom in the Udp-N-Acetylmuramoyl-L-Alanine:D-Glutamate Ligase (pdb code 2uag). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Udp-N-Acetylmuramoyl-L-Alanine:D-Glutamate Ligase, PDB code: 2uag:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in 2uag

Go back to Magnesium Binding Sites List in 2uag
Magnesium binding site 1 out of 2 in the Udp-N-Acetylmuramoyl-L-Alanine:D-Glutamate Ligase


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Udp-N-Acetylmuramoyl-L-Alanine:D-Glutamate Ligase within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg1000

b:4.7
occ:1.00
O20 A:UMA450 1.8 6.3 1.0
O A:HOH1255 2.1 6.1 1.0
O A:HOH1177 2.1 6.9 1.0
O A:HOH1096 2.1 5.4 1.0
NE2 A:HIS183 2.1 4.3 1.0
O A:HOH1173 2.2 5.8 1.0
C22 A:UMA450 2.8 8.1 1.0
CE1 A:HIS183 3.0 6.2 1.0
CD2 A:HIS183 3.3 4.7 1.0
O19 A:UMA450 3.3 8.5 1.0
OD2 A:ASP182 3.6 5.8 1.0
O A:HOH1241 4.0 5.4 1.0
O A:HOH1094 4.0 5.5 1.0
C21 A:UMA450 4.1 7.2 1.0
OQ1 A:KCX198 4.1 3.6 1.0
ND1 A:HIS183 4.2 5.2 1.0
C23 A:UMA450 4.3 6.5 1.0
O A:HOH1174 4.3 4.4 1.0
NZ A:LYS115 4.3 2.9 1.0
OQ2 A:KCX198 4.3 4.3 1.0
CG A:HIS183 4.3 4.7 1.0
CG A:ASP182 4.5 5.4 1.0
CX A:KCX198 4.7 3.8 1.0
OG1 A:THR321 4.8 10.1 1.0
NZ A:LYS348 4.9 4.4 1.0
OD1 A:ASP182 4.9 5.0 1.0

Magnesium binding site 2 out of 2 in 2uag

Go back to Magnesium Binding Sites List in 2uag
Magnesium binding site 2 out of 2 in the Udp-N-Acetylmuramoyl-L-Alanine:D-Glutamate Ligase


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Udp-N-Acetylmuramoyl-L-Alanine:D-Glutamate Ligase within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg1001

b:7.9
occ:1.00
O1B A:ADP451 2.1 5.1 1.0
OG A:SER116 2.1 4.8 1.0
O A:HOH1310 2.1 5.3 1.0
O A:HOH1309 2.1 7.1 1.0
O A:HOH1126 2.2 5.0 1.0
OE2 A:GLU157 2.3 6.4 1.0
CD A:GLU157 3.2 5.8 1.0
PB A:ADP451 3.2 5.5 1.0
CB A:SER116 3.3 3.3 1.0
OE1 A:GLU157 3.4 6.5 1.0
O3B A:ADP451 3.5 5.1 1.0
N A:SER116 3.9 4.1 1.0
O1A A:ADP451 3.9 5.5 1.0
ND2 A:ASN138 4.1 4.6 1.0
CA A:SER116 4.1 4.0 1.0
O A:HOH1212 4.2 28.4 1.0
O A:HOH1311 4.2 25.4 1.0
O2B A:ADP451 4.2 4.0 1.0
O3A A:ADP451 4.3 6.0 1.0
NH2 A:ARG302 4.3 7.2 1.0
O A:ASN138 4.5 8.5 1.0
CG A:GLU157 4.5 4.5 1.0
PA A:ADP451 4.7 6.3 1.0
O A:HOH1313 4.7 15.7 1.0
CE A:LYS115 4.7 2.4 1.0
CB A:LYS115 4.7 3.2 1.0
CA A:ILE139 4.8 8.5 1.0
C A:LYS115 4.9 3.6 1.0
O19 A:UMA450 4.9 8.5 1.0
C A:ASN138 5.0 6.7 1.0

Reference:

J.A.Bertrand, G.Auger, L.Martin, E.Fanchon, D.Blanot, D.Le Beller, J.Van Heijenoort, O.Dideberg. Determination of the Murd Mechanism Through Crystallographic Analysis of Enzyme Complexes. J.Mol.Biol. V. 289 579 1999.
ISSN: ISSN 0022-2836
PubMed: 10356330
DOI: 10.1006/JMBI.1999.2800
Page generated: Mon Dec 14 07:38:47 2020

Last articles

Zn in 7VD8
Zn in 7V1R
Zn in 7V1Q
Zn in 7VPF
Zn in 7T85
Zn in 7T5F
Zn in 7NF9
Zn in 7M4M
Zn in 7M4O
Zn in 7M4N
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy