Magnesium in PDB 2v9j: Crystal Structure of the Regulatory Fragment of Mammalian Ampk in Complexes with Mg.Atp-Amp

All present enzymatic activity of Crystal Structure of the Regulatory Fragment of Mammalian Ampk in Complexes with Mg.Atp-Amp:
2.7.11.1;

The structure of Crystal Structure of the Regulatory Fragment of Mammalian Ampk in Complexes with Mg.Atp-Amp, PDB code: 2v9j was solved by B.Xiao, R.Heath, P.Saiu, F.C.Leiper, P.Leone, C.Jing, P.A.Walker, L.Haire, J.F.Eccleston, C.T.Davis, S.R.Martin, D.Carling, S.J.Gamblin, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 2.53
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	48.790, 120.685, 127.069, 90.00, 90.00, 90.00
R / Rfree (%)	22.5 / 26.6

The binding sites of Magnesium atom in the Crystal Structure of the Regulatory Fragment of Mammalian Ampk in Complexes with Mg.Atp-Amp (pdb code 2v9j). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of the Regulatory Fragment of Mammalian Ampk in Complexes with Mg.Atp-Amp, PDB code: 2v9j:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of the Regulatory Fragment of Mammalian Ampk in Complexes with Mg.Atp-Amp


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of the Regulatory Fragment of Mammalian Ampk in Complexes with Mg.Atp-Amp within 5.0Å range: probe atom residue distance (Å) B Occ E:Mg1330 b:58.7 occ:1.00 O E:HOH2074 2.0 43.6 1.0 O1G E:ATP1327 2.1 59.8 1.0 O2B E:ATP1327 2.1 58.1 1.0 O E:HOH2077 2.1 37.4 1.0 PB E:ATP1327 3.3 58.3 1.0 PG E:ATP1327 3.3 61.1 1.0 O3B E:ATP1327 3.4 60.3 1.0 NZ E:LYS169 3.4 52.1 1.0 O1B E:ATP1327 4.1 58.4 1.0 NH1 E:ARG69 4.1 60.3 1.0 O2G E:ATP1327 4.2 61.4 1.0 CZ E:ARG69 4.2 60.0 1.0 CE E:LYS169 4.2 49.8 1.0 O3G E:ATP1327 4.3 62.1 1.0 NE E:ARG69 4.5 57.9 1.0 O E:HOH2033 4.5 46.0 1.0 O3A E:ATP1327 4.5 55.8 1.0 CD E:LYS169 4.5 48.1 1.0 NH2 E:ARG69 4.5 60.9 1.0 NH1 E:ARG151 4.6 61.7 1.0 CD E:ARG69 4.8 55.9 1.0 CG E:ARG69 4.8 51.6 1.0 NH2 E:ARG151 4.9 60.4 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of the Regulatory Fragment of Mammalian Ampk in Complexes with Mg.Atp-Amp


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of the Regulatory Fragment of Mammalian Ampk in Complexes with Mg.Atp-Amp within 5.0Å range: probe atom residue distance (Å) B Occ E:Mg1331 b:60.6 occ:1.00 O1G E:ATP1328 2.1 61.5 1.0 O E:HOH2078 2.1 51.0 1.0 O2B E:ATP1328 2.1 57.1 1.0 PB E:ATP1328 3.2 53.9 1.0 PG E:ATP1328 3.3 62.1 1.0 CG1 E:ILE87 3.3 45.5 1.0 O3B E:ATP1328 3.3 58.6 1.0 CB E:ILE87 3.5 45.5 1.0 NH2 E:ARG151 3.7 60.4 1.0 O2G E:ATP1328 4.0 62.3 1.0 O1B E:ATP1328 4.1 56.8 1.0 N E:ILE87 4.3 45.3 1.0 O3G E:ATP1328 4.4 63.8 1.0 CZ E:ARG151 4.4 60.8 1.0 CG E:ARG69 4.4 51.6 1.0 CA E:ILE87 4.5 45.0 1.0 CB E:LYS242 4.5 56.6 1.0 CG2 E:ILE87 4.6 44.3 1.0 O3A E:ATP1328 4.6 47.8 1.0 O1B E:ATP1327 4.6 58.4 1.0 O E:HOH2075 4.6 25.4 1.0 CD1 E:ILE87 4.7 47.7 1.0 O E:HOH2076 4.8 13.6 1.0 N E:THR88 4.9 45.1 1.0 CD E:LYS242 4.9 56.5 1.0 NE E:ARG151 5.0 59.4 1.0

B.Xiao, R.Heath, P.Saiu, F.C.Leiper, P.Leone, C.Jing, P.A.Walker, L.Haire, J.F.Eccleston, C.T.Davis, S.R.Martin, D.Carling, S.J.Gamblin. Structural Basis For Amp Binding to Mammalian Amp- Activated Protein Kinase Nature V. 449 496 2007.
ISSN: ISSN 0028-0836
PubMed: 17851531
DOI: 10.1038/NATURE06161