Magnesium in PDB 2w2x: Complex of RAC2 and PLCG2 Spph Domain

All present enzymatic activity of Complex of RAC2 and PLCG2 Spph Domain:
3.1.4.11;

The structure of Complex of RAC2 and PLCG2 Spph Domain, PDB code: 2w2x was solved by O.Opaleye, T.D.Bunney, S.M.Roe, L.H.Pearl, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	69.41 / 2.30
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	131.570, 84.459, 74.196, 90.00, 112.21, 90.00
R / Rfree (%)	23.4 / 29.4

The binding sites of Magnesium atom in the Complex of RAC2 and PLCG2 Spph Domain (pdb code 2w2x). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Complex of RAC2 and PLCG2 Spph Domain, PDB code: 2w2x:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Complex of RAC2 and PLCG2 Spph Domain


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Complex of RAC2 and PLCG2 Spph Domain within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1179 b:47.5 occ:1.00 OG1 A:THR17 2.2 47.1 1.0 OG1 A:THR35 2.3 59.7 1.0 O2G A:GSP1178 2.3 69.6 1.0 O2B A:GSP1178 2.5 37.0 1.0 CB A:THR35 2.9 34.8 1.0 PG A:GSP1178 3.2 50.0 1.0 O3G A:GSP1178 3.2 33.0 1.0 OD1 A:ASP57 3.4 51.7 0.8 O3B A:GSP1178 3.5 0.8 1.0 PB A:GSP1178 3.5 51.8 1.0 OD2 A:ASP57 3.5 42.0 0.8 CB A:THR17 3.5 44.3 1.0 CG2 A:THR35 3.7 34.1 1.0 CG A:ASP57 3.8 57.0 0.8 N A:THR17 3.9 50.1 1.0 O A:THR58 3.9 43.0 0.8 CA A:THR17 4.2 42.4 1.0 CA A:THR35 4.2 41.9 1.0 O1B A:GSP1178 4.2 61.8 1.0 N A:THR35 4.3 42.5 1.0 O2A A:GSP1178 4.4 81.8 1.0 CE A:LYS16 4.5 32.2 1.0 CB A:LYS16 4.5 45.9 1.0 CG2 A:THR17 4.6 46.9 1.0 O3A A:GSP1178 4.7 49.6 1.0 S1G A:GSP1178 4.8 64.9 1.0 NZ A:LYS16 4.8 41.6 1.0 C A:LYS16 4.8 53.1 1.0 O1A A:GSP1178 4.9 40.0 1.0 PA A:GSP1178 5.0 57.0 1.0

Magnesium binding site 2 out of 2 in the Complex of RAC2 and PLCG2 Spph Domain


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Complex of RAC2 and PLCG2 Spph Domain within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg1179 b:46.2 occ:1.00 OG1 B:THR17 2.2 39.2 1.0 O2G B:GSP1178 2.4 60.1 1.0 OG1 B:THR35 2.6 76.3 1.0 O1B B:GSP1178 2.7 35.6 1.0 CB B:THR35 3.1 31.8 1.0 OD1 B:ASP57 3.3 41.5 1.0 PG B:GSP1178 3.4 61.2 1.0 O3G B:GSP1178 3.4 50.8 1.0 OD2 B:ASP57 3.5 45.5 1.0 CB B:THR17 3.6 53.0 1.0 PB B:GSP1178 3.7 55.8 1.0 CG B:ASP57 3.7 63.2 1.0 O3B B:GSP1178 3.8 55.4 1.0 N B:THR17 3.8 58.2 1.0 O B:THR58 3.8 46.2 1.0 CG2 B:THR35 3.8 29.9 1.0 CA B:THR17 4.1 41.9 1.0 CB B:LYS16 4.3 47.1 1.0 CA B:THR35 4.4 45.3 1.0 CE B:LYS16 4.4 40.5 1.0 N B:THR35 4.4 40.0 1.0 O2B B:GSP1178 4.6 60.1 1.0 CG2 B:THR17 4.6 48.5 1.0 C B:LYS16 4.7 59.0 1.0 NZ B:LYS16 4.7 43.7 1.0 O B:HOH2006 4.8 43.8 1.0 O3A B:GSP1178 4.8 32.9 0.7 O2A B:GSP1178 4.9 51.5 1.0 O1A B:GSP1178 4.9 0.8 1.0 S1G B:GSP1178 4.9 59.4 1.0 CA B:LYS16 4.9 42.1 1.0 C B:THR58 5.0 34.8 1.0

T.D.Bunney, O.Opaleye, S.M.Roe, P.Vatter, R.W.Baxendale, C.Walliser, K.L.Everett, M.B.Josephs, C.Christow, F.Rodrigues-Lima, P.Gierschik, L.H.Pearl, M.Katan. Structural Insights Into Formation of An Active Signaling Complex Between Rac and Phospholipase C Gamma 2. Mol.Cell V. 34 223 2009.
ISSN: ISSN 1097-2765
PubMed: 19394299
DOI: 10.1016/J.MOLCEL.2009.02.023