Magnesium in PDB 2wkr: Structure of A Photoactivatable RAC1 Containing the LOV2 C450M Mutant

The structure of Structure of A Photoactivatable RAC1 Containing the LOV2 C450M Mutant, PDB code: 2wkr was solved by Y.I.Wu, D.Frey, O.I.Lungu, A.Jaehrig, I.Schlichting, B.Kuhlman, K.M.Hahn, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	32.60 / 2.20
Space group	P 32 2 1
Cell size a, b, c (Å), α, β, γ (°)	112.390, 112.390, 69.200, 90.00, 90.00, 120.00
R / Rfree (%)	18.6 / 22.6

The structure of Structure of A Photoactivatable RAC1 Containing the LOV2 C450M Mutant also contains other interesting chemical elements:

Chlorine

(Cl)

2 atoms

The binding sites of Magnesium atom in the Structure of A Photoactivatable RAC1 Containing the LOV2 C450M Mutant (pdb code 2wkr). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the Structure of A Photoactivatable RAC1 Containing the LOV2 C450M Mutant, PDB code: 2wkr:

Magnesium binding site 1 out of 1 in the Structure of A Photoactivatable RAC1 Containing the LOV2 C450M Mutant


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Structure of A Photoactivatable RAC1 Containing the LOV2 C450M Mutant within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1724 b:12.6 occ:1.00 O1G A:GTP1722 2.1 9.8 1.0 O1B A:GTP1722 2.1 10.3 1.0 OG1 A:THR560 2.2 10.8 1.0 OG1 A:THR578 2.2 14.8 1.0 O A:HOH2128 2.2 9.1 1.0 O A:HOH2212 2.3 10.9 1.0 CB A:THR578 3.1 14.3 1.0 CB A:THR560 3.2 12.2 1.0 PG A:GTP1722 3.2 14.2 1.0 PB A:GTP1722 3.3 12.6 1.0 O3B A:GTP1722 3.5 12.3 1.0 N A:THR578 3.8 15.8 1.0 N A:THR560 3.9 9.6 1.0 OD2 A:ASP600 3.9 10.7 1.0 O3G A:GTP1722 4.0 9.5 1.0 CA A:THR578 4.1 17.0 1.0 O1A A:GTP1722 4.1 13.7 1.0 CA A:THR560 4.1 11.0 1.0 OD1 A:ASP600 4.2 10.7 1.0 CG2 A:THR578 4.2 10.3 1.0 O2B A:GTP1722 4.3 10.2 1.0 O3A A:GTP1722 4.3 15.6 1.0 CG2 A:THR560 4.3 11.0 1.0 O A:HOH2098 4.3 21.8 1.0 O2G A:GTP1722 4.4 17.3 1.0 O A:ILE576 4.4 18.1 1.0 CG A:ASP600 4.4 10.7 1.0 O A:THR601 4.5 15.1 1.0 PA A:GTP1722 4.6 13.1 1.0 C A:PRO577 4.6 17.9 1.0 O2A A:GTP1722 4.7 15.7 1.0 CB A:LYS559 4.8 10.1 1.0 CA A:PRO577 4.9 19.0 1.0 CE A:LYS559 5.0 11.8 1.0 C A:LYS559 5.0 12.5 1.0

Y.I.Wu, D.Frey, O.I.Lungu, A.Jaehrig, I.Schlichting, B.Kuhlman, K.M.Hahn. A Genetically Encoded Photoactivatable Rac Controls the Motility of Living Cells. Nature V. 461 104 2009.
ISSN: ISSN 0028-0836
PubMed: 19693014
DOI: 10.1038/NATURE08241