Magnesium in PDB 2woj: Adp-ALF4 Complex of S. Cerevisiae GET3

All present enzymatic activity of Adp-ALF4 Complex of S. Cerevisiae GET3:
3.6.3.16;

The structure of Adp-ALF4 Complex of S. Cerevisiae GET3, PDB code: 2woj was solved by A.Mateja, A.Szlachcic, M.E.Downing, M.Dobosz, M.Mariappan, R.S.Hegde, R.J.Keenan, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	37.441 / 1.99
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	53.612, 82.628, 167.282, 90.00, 98.28, 90.00
R / Rfree (%)	17.59 / 21.27

The structure of Adp-ALF4 Complex of S. Cerevisiae GET3 also contains other interesting chemical elements:

Fluorine	(F)	16 atoms
Aluminium	(Al)	4 atoms
Zinc	(Zn)	2 atoms

The binding sites of Magnesium atom in the Adp-ALF4 Complex of S. Cerevisiae GET3 (pdb code 2woj). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 4 binding sites of Magnesium where determined in the Adp-ALF4 Complex of S. Cerevisiae GET3, PDB code: 2woj:
Jump to Magnesium binding site number: 1; 2; 3; 4;

Magnesium binding site 1 out of 4 in the Adp-ALF4 Complex of S. Cerevisiae GET3


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Adp-ALF4 Complex of S. Cerevisiae GET3 within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg403 b:28.8 occ:1.00 F2 A:ALF402 2.0 32.4 1.0 O2B A:ADP401 2.1 22.3 1.0 O A:HOH2133 2.1 24.3 1.0 O A:HOH2025 2.1 22.3 1.0 O A:HOH2066 2.1 29.5 1.0 OG1 A:THR32 2.1 24.3 1.0 F4 A:ALF402 3.1 34.4 1.0 CB A:THR32 3.1 24.7 1.0 AL A:ALF402 3.3 29.1 1.0 PB A:ADP401 3.4 21.0 1.0 O1B A:ADP401 3.5 23.4 1.0 N A:THR32 3.7 26.9 1.0 OD2 A:ASP166 3.9 35.3 1.0 CA A:THR32 4.0 24.8 1.0 OD1 A:ASP166 4.0 29.7 1.0 ND2 A:ASN61 4.0 27.9 1.0 OD2 A:ASP57 4.0 28.8 1.0 O2A A:ADP401 4.2 24.8 1.0 O3A A:ADP401 4.3 21.3 1.0 O A:HOH2021 4.3 26.4 1.0 CG2 A:THR32 4.3 24.8 1.0 O A:HOH2029 4.4 12.9 1.0 CG A:ASP166 4.4 32.0 1.0 O3B A:ADP401 4.4 22.7 1.0 O A:HOH2023 4.4 23.6 1.0 F3 A:ALF402 4.5 25.0 1.0 CB A:LYS31 4.5 21.2 1.0 CE A:LYS31 4.7 23.1 1.0 PA A:ADP401 4.7 24.8 1.0 CB A:ASN61 4.7 24.8 1.0 C A:LYS31 4.8 25.7 1.0 O A:THR167 4.8 23.3 1.0 O1A A:ADP401 4.8 23.2 1.0 F1 A:ALF402 4.9 33.6 1.0 CG A:ASN61 4.9 30.1 1.0 NZ A:LYS31 5.0 21.8 1.0

Magnesium binding site 2 out of 4 in the Adp-ALF4 Complex of S. Cerevisiae GET3


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Adp-ALF4 Complex of S. Cerevisiae GET3 within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg403 b:27.4 occ:1.00 F2 B:ALF402 2.0 29.0 1.0 O2B B:ADP401 2.1 21.6 1.0 O B:HOH2154 2.1 25.2 1.0 O B:HOH2078 2.1 27.2 1.0 O B:HOH2031 2.1 24.0 1.0 OG1 B:THR32 2.1 24.6 1.0 F4 B:ALF402 3.0 35.2 1.0 CB B:THR32 3.1 23.4 1.0 O1B B:ADP401 3.3 20.8 1.0 AL B:ALF402 3.3 27.9 1.0 PB B:ADP401 3.4 22.3 1.0 N B:THR32 3.7 23.9 1.0 CA B:THR32 4.0 22.1 1.0 OD2 B:ASP57 4.0 29.2 1.0 OD1 B:ASP166 4.1 29.3 1.0 O B:HOH2079 4.1 27.6 1.0 ND2 B:ASN61 4.1 35.5 1.0 OD2 B:ASP166 4.2 30.9 1.0 O2A B:ADP401 4.2 24.7 1.0 O3A B:ADP401 4.2 21.6 1.0 O A:HOH2090 4.3 14.7 1.0 O3B B:ADP401 4.3 25.4 1.0 CG2 B:THR32 4.4 24.0 1.0 O B:HOH2032 4.4 25.5 1.0 F3 B:ALF402 4.5 25.1 1.0 CG B:ASP166 4.5 29.7 1.0 CB B:LYS31 4.6 24.6 1.0 CB B:ASN61 4.7 26.9 1.0 PA B:ADP401 4.7 23.2 1.0 C B:LYS31 4.7 24.7 1.0 CE B:LYS31 4.8 22.1 1.0 O1A B:ADP401 4.9 24.6 1.0 F1 B:ALF402 4.9 31.7 1.0 O B:THR167 4.9 22.9 1.0 CG B:ASN61 4.9 30.8 1.0

Magnesium binding site 3 out of 4 in the Adp-ALF4 Complex of S. Cerevisiae GET3


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Adp-ALF4 Complex of S. Cerevisiae GET3 within 5.0Å range: probe atom residue distance (Å) B Occ C:Mg403 b:30.4 occ:1.00 F2 C:ALF402 2.0 28.7 1.0 O2B C:ADP401 2.1 28.5 1.0 OG1 C:THR32 2.1 27.4 1.0 O C:HOH2033 2.1 25.2 1.0 O C:HOH2026 2.1 28.4 1.0 O C:HOH2066 2.1 29.9 1.0 F4 C:ALF402 2.9 41.7 1.0 CB C:THR32 3.2 26.5 1.0 AL C:ALF402 3.3 29.6 1.0 PB C:ADP401 3.4 25.4 1.0 O1B C:ADP401 3.4 25.9 1.0 N C:THR32 3.7 27.0 1.0 ND2 C:ASN61 3.9 31.9 1.0 OD1 C:ASP166 4.0 34.6 1.0 CA C:THR32 4.0 28.2 1.0 OD2 C:ASP166 4.1 34.2 1.0 OD2 C:ASP57 4.1 29.1 1.0 O2A C:ADP401 4.2 28.3 1.0 O3A C:ADP401 4.3 24.3 1.0 O C:HOH2021 4.3 28.2 1.0 O3B C:ADP401 4.3 29.3 1.0 CG2 C:THR32 4.3 27.6 1.0 O C:HOH2034 4.4 15.2 1.0 O C:HOH2027 4.4 30.6 1.0 CG C:ASP166 4.5 34.7 1.0 F3 C:ALF402 4.5 29.7 1.0 CB C:ASN61 4.6 26.8 1.0 PA C:ADP401 4.7 27.4 1.0 CB C:LYS31 4.7 25.8 1.0 O1A C:ADP401 4.8 26.8 1.0 CE C:LYS31 4.8 30.1 1.0 C C:LYS31 4.8 28.9 1.0 CG C:ASN61 4.8 36.5 1.0 F1 C:ALF402 4.8 33.6 1.0 O C:THR167 4.9 26.9 1.0

Magnesium binding site 4 out of 4 in the Adp-ALF4 Complex of S. Cerevisiae GET3


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 4 of Adp-ALF4 Complex of S. Cerevisiae GET3 within 5.0Å range: probe atom residue distance (Å) B Occ D:Mg403 b:35.3 occ:1.00 F2 D:ALF402 2.0 30.7 1.0 O2B D:ADP401 2.1 29.5 1.0 O D:HOH2028 2.1 29.9 1.0 OG1 D:THR32 2.1 33.1 1.0 O D:HOH2055 2.1 34.9 1.0 O D:HOH2022 2.1 29.7 1.0 F4 D:ALF402 3.1 41.1 1.0 CB D:THR32 3.2 32.3 1.0 PB D:ADP401 3.3 29.2 1.0 O1B D:ADP401 3.3 28.8 1.0 AL D:ALF402 3.3 32.2 1.0 N D:THR32 3.8 30.9 1.0 ND2 D:ASN61 3.9 36.2 1.0 OD2 D:ASP57 4.0 32.4 1.0 CA D:THR32 4.0 31.3 1.0 OD1 D:ASP166 4.1 39.8 1.0 O2A D:ADP401 4.1 30.2 1.0 OD2 D:ASP166 4.1 36.8 1.0 O3A D:ADP401 4.2 29.7 1.0 O3B D:ADP401 4.3 37.7 1.0 O D:HOH2029 4.3 16.4 1.0 O D:HOH2054 4.4 32.2 1.0 F3 D:ALF402 4.4 28.7 1.0 O D:HOH2023 4.4 29.6 1.0 CG2 D:THR32 4.4 39.2 1.0 CG D:ASP166 4.5 38.1 1.0 PA D:ADP401 4.6 30.4 1.0 CB D:ASN61 4.6 33.4 1.0 CB D:LYS31 4.7 30.0 1.0 O1A D:ADP401 4.7 30.8 1.0 CE D:LYS31 4.8 35.8 1.0 CG D:ASN61 4.8 38.7 1.0 C D:LYS31 4.9 31.0 1.0 F1 D:ALF402 4.9 37.2 1.0 O D:THR167 4.9 29.4 1.0

A.Mateja, A.Szlachcic, M.E.Downing, M.Dobosz, M.Mariappan, R.S.Hegde, R.J.Keenan. The Structural Basis of Tail-Anchored Membrane Protein Recognition By GET3. Nature V. 461 361 2009.
ISSN: ISSN 0028-0836
PubMed: 19675567
DOI: 10.1038/NATURE08319