Magnesium in PDB 2xkb: Crystal Structure of Gdp-Form Protofilaments of Bacillus Thuringiensis Serovar Israelensis Tubz

The structure of Crystal Structure of Gdp-Form Protofilaments of Bacillus Thuringiensis Serovar Israelensis Tubz, PDB code: 2xkb was solved by C.H.S.Aylett, J.Lowe, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.00 / 3.00
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	52.947, 541.099, 86.128, 90.00, 92.51, 90.00
R / Rfree (%)	23.4 / 29.4

The binding sites of Magnesium atom in the Crystal Structure of Gdp-Form Protofilaments of Bacillus Thuringiensis Serovar Israelensis Tubz (pdb code 2xkb). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 4 binding sites of Magnesium where determined in the Crystal Structure of Gdp-Form Protofilaments of Bacillus Thuringiensis Serovar Israelensis Tubz, PDB code: 2xkb:
Jump to Magnesium binding site number: 1; 2; 3; 4;

Magnesium binding site 1 out of 4 in the Crystal Structure of Gdp-Form Protofilaments of Bacillus Thuringiensis Serovar Israelensis Tubz


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of Gdp-Form Protofilaments of Bacillus Thuringiensis Serovar Israelensis Tubz within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg999 b:12.9 occ:1.00 OD2 A:ASP64 2.2 48.3 1.0 O1B A:GDP900 2.6 50.0 1.0 CG A:ASP64 3.2 48.1 1.0 O3A A:GDP900 3.4 49.7 1.0 OD1 A:ASP64 3.4 47.9 1.0 PB A:GDP900 3.5 49.6 1.0 O2B A:GDP900 4.0 49.7 1.0 NE2 A:GLN32 4.2 42.7 1.0 CG2 A:VAL141 4.4 49.8 1.0 CB A:GLN32 4.6 41.3 1.0 CB A:ASP64 4.6 48.4 1.0 PA A:GDP900 4.7 49.5 1.0 CB A:ALA61 4.8 50.2 1.0 O3B A:GDP900 4.8 48.7 1.0 N A:GLN32 4.8 41.1 1.0 ND2 A:ASN59 5.0 51.8 1.0

Magnesium binding site 2 out of 4 in the Crystal Structure of Gdp-Form Protofilaments of Bacillus Thuringiensis Serovar Israelensis Tubz


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of Gdp-Form Protofilaments of Bacillus Thuringiensis Serovar Israelensis Tubz within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg999 b:13.7 occ:1.00 O1B B:GDP900 2.4 40.4 1.0 OD2 B:ASP64 2.5 53.9 1.0 OD1 A:ASP266 3.0 50.9 1.0 O2B B:GDP900 3.1 38.1 1.0 NZ A:LYS359 3.3 53.5 1.0 CG B:ASP64 3.3 53.1 1.0 OD1 B:ASP64 3.4 54.2 1.0 PB B:GDP900 3.4 39.2 1.0 O1A B:GDP900 3.8 39.2 1.0 CB B:GLN32 4.1 42.5 1.0 O A:THR264 4.2 45.9 1.0 CG A:ASP266 4.3 49.7 1.0 O3B B:GDP900 4.4 38.7 1.0 O3A B:GDP900 4.6 39.3 1.0 N B:GLN32 4.6 42.0 1.0 CB B:ALA61 4.6 54.6 1.0 CE A:LYS359 4.7 52.5 1.0 PA B:GDP900 4.8 40.1 1.0 CB B:ASP64 4.8 52.3 1.0 OE1 B:GLN32 4.8 44.8 1.0 ND2 B:ASN59 4.9 51.8 1.0 CD B:GLN32 5.0 44.9 1.0 CA B:GLN32 5.0 42.1 1.0 CG B:GLN32 5.0 43.8 1.0

Magnesium binding site 3 out of 4 in the Crystal Structure of Gdp-Form Protofilaments of Bacillus Thuringiensis Serovar Israelensis Tubz


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Crystal Structure of Gdp-Form Protofilaments of Bacillus Thuringiensis Serovar Israelensis Tubz within 5.0Å range: probe atom residue distance (Å) B Occ G:Mg999 b:17.9 occ:1.00 O1B G:GDP900 2.5 39.4 1.0 OD2 G:ASP64 2.7 50.5 1.0 OD1 H:ASP266 3.1 50.9 1.0 CG G:ASP64 3.2 50.4 1.0 OD1 G:ASP64 3.2 49.7 1.0 O2B G:GDP900 3.3 37.6 1.0 PB G:GDP900 3.5 38.0 1.0 O1A G:GDP900 4.0 37.4 1.0 NZ H:LYS359 4.1 53.5 1.0 NE2 G:GLN32 4.2 40.4 1.0 CG H:ASP266 4.4 49.7 1.0 OD1 G:ASN59 4.4 52.0 1.0 ND2 G:ASN59 4.5 51.7 1.0 CB G:GLN32 4.5 41.7 1.0 CB G:ASP64 4.5 50.3 1.0 N G:GLN32 4.5 41.4 1.0 O3B G:GDP900 4.5 37.9 1.0 O3A G:GDP900 4.6 37.8 1.0 O H:THR264 4.7 41.2 1.0 PA G:GDP900 4.9 38.0 1.0 CG G:ASN59 4.9 51.5 1.0 CG2 G:VAL141 4.9 46.5 1.0 CD G:GLN32 5.0 41.0 1.0

Magnesium binding site 4 out of 4 in the Crystal Structure of Gdp-Form Protofilaments of Bacillus Thuringiensis Serovar Israelensis Tubz


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 4 of Crystal Structure of Gdp-Form Protofilaments of Bacillus Thuringiensis Serovar Israelensis Tubz within 5.0Å range: probe atom residue distance (Å) B Occ I:Mg999 b:17.8 occ:1.00 O1B I:GDP900 2.5 30.6 1.0 OD2 I:ASP64 2.6 50.2 1.0 OD1 J:ASP266 3.1 50.8 1.0 O2B I:GDP900 3.3 29.1 1.0 NZ J:LYS359 3.3 53.5 1.0 OD1 I:ASP64 3.4 49.8 1.0 CG I:ASP64 3.4 49.1 1.0 PB I:GDP900 3.5 31.5 1.0 CG I:GLN32 3.8 43.0 1.0 NE2 I:GLN32 3.9 44.0 1.0 O1A I:GDP900 4.0 27.1 1.0 CD I:GLN32 4.3 44.6 1.0 CG J:ASP266 4.4 49.7 1.0 O3A I:GDP900 4.4 29.6 1.0 CB I:ALA61 4.6 51.2 1.0 CE J:LYS359 4.7 52.4 1.0 PA I:GDP900 4.7 27.9 1.0 N I:GLN32 4.8 40.9 1.0 CB I:GLN32 4.8 41.3 1.0 O3B I:GDP900 4.8 28.8 1.0 O J:THR264 4.8 45.4 1.0 CB I:ASP64 4.9 48.6 1.0 OD1 I:ASN59 4.9 51.8 1.0 ND2 I:ASN59 4.9 51.7 1.0

C.H.Aylett, Q.Wang, K.A.Michie, L.A.Amos, J.Lowe. Filament Structure of Bacterial Tubulin Homologue Tubz. Proc.Natl.Acad.Sci.Usa V. 107 19766 2010.
ISSN: ISSN 0027-8424
PubMed: 20974911
DOI: 10.1073/PNAS.1010176107