Magnesium in PDB 2xzv: The Cyanobacterial PP2C-Like Phosphatase Tppha Requires Three Metals in the Catalytic Center For Efficient Catalysis

The structure of The Cyanobacterial PP2C-Like Phosphatase Tppha Requires Three Metals in the Catalytic Center For Efficient Catalysis, PDB code: 2xzv was solved by C.Schlicker, S.Jiyong, K.Forchhammer, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	36.258 / 1.60
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	38.646, 151.436, 82.603, 90.00, 90.00, 90.00
R / Rfree (%)	20.98 / 24.16

The structure of The Cyanobacterial PP2C-Like Phosphatase Tppha Requires Three Metals in the Catalytic Center For Efficient Catalysis also contains other interesting chemical elements:

Calcium

(Ca)

2 atoms

The binding sites of Magnesium atom in the The Cyanobacterial PP2C-Like Phosphatase Tppha Requires Three Metals in the Catalytic Center For Efficient Catalysis (pdb code 2xzv). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the The Cyanobacterial PP2C-Like Phosphatase Tppha Requires Three Metals in the Catalytic Center For Efficient Catalysis, PDB code: 2xzv:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the The Cyanobacterial PP2C-Like Phosphatase Tppha Requires Three Metals in the Catalytic Center For Efficient Catalysis


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of The Cyanobacterial PP2C-Like Phosphatase Tppha Requires Three Metals in the Catalytic Center For Efficient Catalysis within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1243 b:34.9 occ:1.00 O A:HOH2083 2.4 39.0 1.0 OE1 A:GLU198 2.5 28.8 1.0 O A:HOH2082 2.6 35.7 1.0 ND1 A:HIS227 2.6 30.4 0.5 O A:HIS227 2.6 29.2 1.0 CE1 A:HIS227 2.8 27.3 0.5 CG A:HIS227 3.6 28.5 0.5 CD A:GLU198 3.6 30.1 1.0 C A:HIS227 3.7 27.5 1.0 NE2 A:HIS227 3.7 31.1 0.5 OE2 A:GLU198 4.0 32.2 1.0 CD2 A:HIS227 4.1 28.6 0.5 CB A:HIS227 4.3 26.7 0.5 CB A:HIS227 4.4 26.7 0.5 N A:GLY228 4.5 29.1 1.0 CA A:GLY228 4.5 26.7 1.0 CA A:HIS227 4.6 26.2 1.0 CA A:GLU198 4.7 23.3 1.0 O A:GLU198 4.8 27.0 1.0 CG A:GLU198 4.8 28.2 1.0 O A:GLU197 4.9 29.7 1.0 CB A:GLU198 4.9 26.5 1.0

Magnesium binding site 2 out of 2 in the The Cyanobacterial PP2C-Like Phosphatase Tppha Requires Three Metals in the Catalytic Center For Efficient Catalysis


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of The Cyanobacterial PP2C-Like Phosphatase Tppha Requires Three Metals in the Catalytic Center For Efficient Catalysis within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1244 b:43.8 occ:1.00 OE2 A:GLU183 2.5 33.7 1.0 OE1 A:GLU183 2.8 32.8 1.0 CD A:GLU183 3.0 32.5 1.0 CG A:GLU183 4.5 29.6 1.0 CD A:PRO184 4.7 29.4 1.0 O A:HOH2076 4.8 31.0 1.0 O A:LEU182 4.9 27.3 1.0

J.Su, C.Schlicker, K.Forchhammer. A Third Metal Is Required For Catalytic Activity of the Signal-Transducing Protein Phosphatase M Tppha. J.Biol.Chem. V. 286 13481 2011.
ISSN: ISSN 0021-9258
PubMed: 21310952
DOI: 10.1074/JBC.M109.036467