Magnesium in PDB 2y09: The Cyanobacterial PP2C-Like Phosphatase Tppha Requires Three Metals in the Catalytic Center For Efficient Catalysis

The structure of The Cyanobacterial PP2C-Like Phosphatase Tppha Requires Three Metals in the Catalytic Center For Efficient Catalysis, PDB code: 2y09 was solved by C.Schlicker, S.Jiyong, K.Forchhammer, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	36.330 / 1.70
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	38.714, 151.678, 82.777, 90.00, 90.00, 90.00
R / Rfree (%)	19.23 / 21.85

The structure of The Cyanobacterial PP2C-Like Phosphatase Tppha Requires Three Metals in the Catalytic Center For Efficient Catalysis also contains other interesting chemical elements:

Calcium

(Ca)

2 atoms

The binding sites of Magnesium atom in the The Cyanobacterial PP2C-Like Phosphatase Tppha Requires Three Metals in the Catalytic Center For Efficient Catalysis (pdb code 2y09). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the The Cyanobacterial PP2C-Like Phosphatase Tppha Requires Three Metals in the Catalytic Center For Efficient Catalysis, PDB code: 2y09:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the The Cyanobacterial PP2C-Like Phosphatase Tppha Requires Three Metals in the Catalytic Center For Efficient Catalysis


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of The Cyanobacterial PP2C-Like Phosphatase Tppha Requires Three Metals in the Catalytic Center For Efficient Catalysis within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1243 b:24.9 occ:1.00 OE2 A:GLU183 2.5 22.2 1.0 OE1 A:GLU183 2.7 23.0 1.0 CD A:GLU183 3.0 21.7 1.0 CG A:GLU183 4.4 21.2 1.0 O A:HOH2082 4.6 27.8 1.0 O A:HOH2083 4.6 18.9 1.0 CD A:PRO184 4.7 19.6 1.0 O A:LEU182 4.8 19.2 1.0

Magnesium binding site 2 out of 2 in the The Cyanobacterial PP2C-Like Phosphatase Tppha Requires Three Metals in the Catalytic Center For Efficient Catalysis


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of The Cyanobacterial PP2C-Like Phosphatase Tppha Requires Three Metals in the Catalytic Center For Efficient Catalysis within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1244 b:27.4 occ:1.00 O A:HOH2093 2.5 40.5 1.0 OE1 A:GLU198 2.5 21.0 1.0 O A:HOH2092 2.6 33.2 1.0 CD2 A:HIS227 2.6 21.6 0.5 O A:HIS227 2.7 23.8 1.0 NE2 A:HIS227 2.8 23.0 0.5 CG A:HIS227 3.6 21.7 0.5 CD A:GLU198 3.6 22.9 1.0 C A:HIS227 3.7 21.6 1.0 CE1 A:HIS227 3.8 24.0 0.5 OE2 A:GLU198 4.0 24.9 1.0 ND1 A:HIS227 4.2 21.6 0.5 CA A:GLY228 4.4 22.2 1.0 N A:GLY228 4.5 21.8 1.0 CB A:HIS227 4.5 21.5 0.5 CB A:HIS227 4.5 21.5 0.5 CA A:HIS227 4.7 21.4 1.0 CA A:GLU198 4.7 17.9 1.0 O A:GLU197 4.9 24.4 1.0 CG A:GLU198 4.9 19.9 1.0 O A:GLU198 4.9 19.3 1.0 CB A:GLU198 5.0 17.7 1.0

J.Su, C.Schlicker, K.Forchhammer. A Third Metal Is Required For Catalytic Activity of the Signal-Transducing Protein Phosphatase M Tppha. J.Biol.Chem. V. 286 13481 2011.
ISSN: ISSN 0021-9258
PubMed: 21310952
DOI: 10.1074/JBC.M109.036467