Magnesium in PDB 2y0s: Crystal Structure of Sulfolobus Shibatae Rna Polymerase in P21 Space Group

All present enzymatic activity of Crystal Structure of Sulfolobus Shibatae Rna Polymerase in P21 Space Group:
2.7.7.6;

The structure of Crystal Structure of Sulfolobus Shibatae Rna Polymerase in P21 Space Group, PDB code: 2y0s was solved by M.Wojtas, B.Peralta, M.Ondiviela, M.Mogni, S.D.Bell, N.G.A.Abrescia, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	29.86 / 3.80
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	129.330, 195.909, 212.451, 90.00, 105.73, 90.00
R / Rfree (%)	30 / 35.4

The structure of Crystal Structure of Sulfolobus Shibatae Rna Polymerase in P21 Space Group also contains other interesting chemical elements:

Iron	(Fe)	6 atoms
Zinc	(Zn)	14 atoms

The binding sites of Magnesium atom in the Crystal Structure of Sulfolobus Shibatae Rna Polymerase in P21 Space Group (pdb code 2y0s). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of Sulfolobus Shibatae Rna Polymerase in P21 Space Group, PDB code: 2y0s:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of Sulfolobus Shibatae Rna Polymerase in P21 Space Group


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of Sulfolobus Shibatae Rna Polymerase in P21 Space Group within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1882 b:1.0 occ:1.00 OD1 A:ASP456 1.8 31.1 1.0 OD1 A:ASP458 2.0 43.5 1.0 OD1 A:ASP460 2.2 28.6 1.0 OD2 A:ASP456 2.2 31.1 1.0 OD2 A:ASP458 2.3 42.9 1.0 CG A:ASP456 2.3 31.3 1.0 CG A:ASP458 2.5 43.5 1.0 CG A:ASP460 3.2 29.1 1.0 OD2 A:ASP460 3.6 29.0 1.0 CB A:ASP456 3.9 31.8 1.0 CB A:ASP458 4.1 44.6 1.0 CB A:ASP460 4.6 30.2 1.0 N A:ASP458 4.6 45.7 1.0 C A:ASP458 4.6 45.6 1.0 CA A:ASP458 4.7 45.7 1.0 N A:ASP460 4.7 30.6 1.0 O A:ASP456 4.8 26.5 1.0 O A:ASP458 4.8 45.2 1.0 CA A:ASP456 4.9 26.4 1.0 N A:GLY459 4.9 0.1 1.0 C A:ASP456 4.9 26.4 1.0 CA A:ASP460 4.9 30.6 1.0 C A:GLY459 4.9 0.0 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of Sulfolobus Shibatae Rna Polymerase in P21 Space Group


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of Sulfolobus Shibatae Rna Polymerase in P21 Space Group within 5.0Å range: probe atom residue distance (Å) B Occ W:Mg1882 b:1.0 occ:1.00 OD1 W:ASP456 1.8 47.5 1.0 OD1 W:ASP458 2.0 46.8 1.0 OD2 W:ASP456 2.2 48.3 1.0 OD1 W:ASP460 2.2 59.8 1.0 CG W:ASP456 2.3 47.8 1.0 OD2 W:ASP458 2.4 46.9 1.0 CG W:ASP458 2.5 46.6 1.0 CG W:ASP460 3.2 60.5 1.0 OD2 W:ASP460 3.5 60.6 1.0 CB W:ASP456 3.8 47.6 1.0 CB W:ASP458 4.0 46.6 1.0 CB W:ASP460 4.5 61.4 1.0 N W:ASP458 4.6 46.8 1.0 C W:ASP458 4.6 46.7 1.0 CA W:ASP458 4.7 46.8 1.0 N W:ASP460 4.7 61.2 1.0 O W:ASP456 4.7 48.0 1.0 CA W:ASP456 4.8 48.0 1.0 O W:ASP458 4.8 46.7 1.0 C W:ASP456 4.8 47.9 1.0 CA W:ASP460 4.9 61.6 1.0 N W:GLY459 4.9 0.2 1.0 C W:GLY459 5.0 0.7 1.0 N W:ASP456 5.0 48.5 1.0

M.Wojtas, B.Peralta, M.Ondiviela, M.Mogni, S.D.Bell, N.G.Abrescia. Archaeal Rna Polymerase: the Influence of the Protruding Stalk in Crystal Packing and Preliminary Biophysical Analysis of the RPO13 Subunit. Biochem. Soc. Trans. V. 39 25 2011.
ISSN: ISSN 1470-8752
PubMed: 21265742
DOI: 10.1042/BST0390025