Magnesium in PDB 2zox: Crystal Structure of the Covalent Intermediate of Human Cytosolic Beta-Glucosidase

All present enzymatic activity of Crystal Structure of the Covalent Intermediate of Human Cytosolic Beta-Glucosidase:
3.2.1.21;

The structure of Crystal Structure of the Covalent Intermediate of Human Cytosolic Beta-Glucosidase, PDB code: 2zox was solved by J.Noguchi, Y.Hayashi, Y.Baba, N.Okino, M.Kimura, M.Ito, Y.Kakuta, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.67 / 1.90
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	66.120, 82.529, 91.673, 90.00, 90.00, 90.00
R / Rfree (%)	19.1 / 22.6

The binding sites of Magnesium atom in the Crystal Structure of the Covalent Intermediate of Human Cytosolic Beta-Glucosidase (pdb code 2zox). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of the Covalent Intermediate of Human Cytosolic Beta-Glucosidase, PDB code: 2zox:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of the Covalent Intermediate of Human Cytosolic Beta-Glucosidase


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of the Covalent Intermediate of Human Cytosolic Beta-Glucosidase within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg472 b:11.5 occ:1.00 OG1 A:THR372 3.1 15.4 1.0 O A:HOH487 3.1 18.8 1.0 NH2 A:ARG75 3.2 14.7 1.0 NE A:ARG75 3.2 17.9 1.0 OG A:SER223 3.3 19.5 1.0 CB A:THR372 3.4 16.6 1.0 OE1 A:GLN307 3.4 19.2 1.0 CB A:SER223 3.5 18.1 1.0 CZ A:ARG75 3.6 15.6 1.0 ND2 A:ASN164 3.7 16.3 1.0 CG1 A:ILE161 4.0 14.2 1.0 CG2 A:THR372 4.0 16.2 1.0 OG1 A:THR117 4.0 19.3 1.0 O A:HOH517 4.1 21.2 1.0 CG2 A:ILE161 4.2 15.0 1.0 CD A:ARG75 4.3 15.4 1.0 CG A:ARG75 4.4 16.1 1.0 CB A:ILE161 4.5 16.2 1.0 CD A:GLN307 4.5 20.2 1.0 CD1 A:ILE161 4.6 14.8 1.0 CA A:THR372 4.8 16.9 1.0 O A:THR372 4.8 15.8 1.0 CG A:ASN164 4.9 19.9 1.0 NH1 A:ARG75 4.9 15.8 1.0 CA A:SER223 4.9 18.2 1.0 C A:THR372 5.0 16.7 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of the Covalent Intermediate of Human Cytosolic Beta-Glucosidase


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of the Covalent Intermediate of Human Cytosolic Beta-Glucosidase within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg473 b:34.2 occ:1.00 O A:ARG285 2.5 25.0 1.0 O A:THR188 2.7 23.5 1.0 O A:HOH604 2.9 27.2 1.0 C A:THR188 3.5 23.5 1.0 CA A:THR188 3.5 24.1 1.0 C A:ARG285 3.5 25.3 1.0 CB A:THR188 3.7 23.7 1.0 CA A:ARG285 3.9 25.2 1.0 CB A:GLN192 4.0 20.3 0.6 N A:GLN192 4.0 21.0 1.0 CB A:ARG285 4.2 24.9 1.0 OE1 A:GLN192 4.2 19.3 0.6 CB A:TYR191 4.3 21.9 1.0 CG2 A:THR188 4.4 23.8 1.0 CA A:GLN192 4.4 20.5 0.6 CD A:PRO287 4.6 26.6 1.0 C A:TYR191 4.7 21.5 1.0 N A:GLY189 4.7 23.2 1.0 N A:LEU286 4.7 25.6 1.0 CG A:PRO287 4.8 26.9 1.0 O A:GLY187 4.9 25.5 1.0 N A:THR188 4.9 24.6 1.0 CG A:ARG285 4.9 25.1 1.0 CG A:GLN192 4.9 19.4 0.6 OG1 A:THR188 4.9 23.6 1.0 CA A:TYR191 5.0 21.8 1.0 CD2 A:TYR191 5.0 22.1 1.0 CD A:GLN192 5.0 19.8 0.6

J.Noguchi, Y.Hayashi, Y.Baba, N.Okino, M.Kimura, M.Ito, Y.Kakuta. Crystal Structure of the Covalent Intermediate of Human Cytosolic Beta-Glucosidase Biochem.Biophys.Res.Commun. V. 374 549 2008.
ISSN: ISSN 0006-291X
PubMed: 18662675
DOI: 10.1016/J.BBRC.2008.07.089