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Magnesium in PDB 2zuu: Crystal Structure of Galacto-N-Biose/Lacto-N-Biose I Phosphorylase in Complex with Glcnac

Enzymatic activity of Crystal Structure of Galacto-N-Biose/Lacto-N-Biose I Phosphorylase in Complex with Glcnac

All present enzymatic activity of Crystal Structure of Galacto-N-Biose/Lacto-N-Biose I Phosphorylase in Complex with Glcnac:
2.4.1.211;

Protein crystallography data

The structure of Crystal Structure of Galacto-N-Biose/Lacto-N-Biose I Phosphorylase in Complex with Glcnac, PDB code: 2zuu was solved by M.Hidaka, M.Nishimoto, M.Kitaoka, T.Wakagi, H.Shoun, S.Fushinobu, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 40.68 / 2.30
Space group P 1
Cell size a, b, c (Å), α, β, γ (°) 67.777, 111.484, 118.432, 105.19, 90.48, 107.35
R / Rfree (%) 17 / 23.1

Magnesium Binding Sites:

The binding sites of Magnesium atom in the Crystal Structure of Galacto-N-Biose/Lacto-N-Biose I Phosphorylase in Complex with Glcnac (pdb code 2zuu). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the Crystal Structure of Galacto-N-Biose/Lacto-N-Biose I Phosphorylase in Complex with Glcnac, PDB code: 2zuu:

Magnesium binding site 1 out of 1 in 2zuu

Go back to Magnesium Binding Sites List in 2zuu
Magnesium binding site 1 out of 1 in the Crystal Structure of Galacto-N-Biose/Lacto-N-Biose I Phosphorylase in Complex with Glcnac


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of Galacto-N-Biose/Lacto-N-Biose I Phosphorylase in Complex with Glcnac within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Mg4009

b:36.8
occ:1.00
 O B:HOH1556 2.1 28.1 1.0
O B:HOH1325 2.1 37.2 1.0
O B:HOH1238 2.2 32.5 1.0
O B:HOH1437 2.3 32.0 1.0
OD1 B:ASP132 3.9 32.0 1.0
OD2 B:ASP132 3.9 29.2 1.0
OD2 B:ASP134 4.1 38.7 1.0
CG B:ASP132 4.3 30.5 1.0
CB B:ASP134 4.9 35.5 1.0
O B:HOH1221 4.9 36.2 1.0
CG B:ASP134 4.9 37.9 1.0

Reference:

M.Hidaka, M.Nishimoto, M.Kitaoka, T.Wakagi, H.Shoun, S.Fushinobu. The Crystal Structure of Galacto-N-Biose/Lacto-N-Biose I Phosphorylase: A Large Deformation of A Tim Barrel Scaffold J.Biol.Chem. V. 284 7273 2009.
ISSN: ISSN 0021-9258
PubMed: 19124470
DOI: 10.1074/JBC.M808525200
Page generated: Wed Aug 14 08:02:55 2024

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