Magnesium in PDB 3cif: Crystal Structure of C153S Mutant Glyceraldehyde 3-Phosphate Dehydrogenase From Cryptosporidium Parvum

All present enzymatic activity of Crystal Structure of C153S Mutant Glyceraldehyde 3-Phosphate Dehydrogenase From Cryptosporidium Parvum:
1.2.1.12;

The structure of Crystal Structure of C153S Mutant Glyceraldehyde 3-Phosphate Dehydrogenase From Cryptosporidium Parvum, PDB code: 3cif was solved by W.J.Cook, O.Senkovich, D.Chattopadhyay, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 2.00
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	68.000, 120.100, 79.280, 90.00, 92.08, 90.00
R / Rfree (%)	17.8 / 21

The binding sites of Magnesium atom in the Crystal Structure of C153S Mutant Glyceraldehyde 3-Phosphate Dehydrogenase From Cryptosporidium Parvum (pdb code 3cif). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of C153S Mutant Glyceraldehyde 3-Phosphate Dehydrogenase From Cryptosporidium Parvum, PDB code: 3cif:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of C153S Mutant Glyceraldehyde 3-Phosphate Dehydrogenase From Cryptosporidium Parvum


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of C153S Mutant Glyceraldehyde 3-Phosphate Dehydrogenase From Cryptosporidium Parvum within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg341 b:24.9 occ:1.00 O B:ILE27 2.2 22.1 1.0 O B:ARG24 2.3 27.1 1.0 O B:CYS21 2.4 23.8 1.0 O B:HOH351 3.2 18.4 1.0 C B:ARG24 3.4 27.0 1.0 C B:CYS21 3.4 23.6 1.0 C B:ILE27 3.4 22.2 1.0 CA B:THR28 4.1 21.0 1.0 CA B:ARG24 4.2 27.2 1.0 N B:ARG24 4.2 27.3 1.0 N B:THR28 4.2 21.5 1.0 N B:MET22 4.2 24.7 1.0 CA B:MET22 4.2 25.7 1.0 CA B:CYS21 4.3 22.9 1.0 N B:ASN25 4.3 26.8 1.0 NZ B:LYS71 4.4 31.0 1.0 CB B:ARG24 4.4 27.3 1.0 N B:ILE27 4.5 23.4 1.0 CB B:CYS21 4.5 22.6 1.0 CA B:ILE27 4.5 22.8 1.0 OG1 B:THR28 4.5 22.0 1.0 C B:MET22 4.5 26.1 1.0 CA B:ASN25 4.6 26.6 1.0 CB B:THR28 4.7 21.2 1.0 CG2 B:THR28 4.7 21.6 1.0 O B:MET22 4.7 26.3 1.0 CG2 B:ILE27 4.8 23.1 1.0 C B:ASN25 4.9 26.0 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of C153S Mutant Glyceraldehyde 3-Phosphate Dehydrogenase From Cryptosporidium Parvum


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of C153S Mutant Glyceraldehyde 3-Phosphate Dehydrogenase From Cryptosporidium Parvum within 5.0Å range: probe atom residue distance (Å) B Occ D:Mg340 b:43.9 occ:1.00 O3P D:G3H1340 2.0 33.7 1.0 O D:HOH342 2.6 34.6 1.0 O1P D:G3H1340 3.0 34.7 1.0 P D:G3H1340 3.1 34.1 1.0 OG1 D:THR214 3.3 23.9 1.0 OG1 D:THR154 3.6 13.6 1.0 OG D:SER152 3.7 15.8 1.0 CB D:SER152 3.8 15.3 1.0 CB D:THR154 3.9 13.9 1.0 O2P D:G3H1340 3.9 34.1 1.0 O D:HOH519 4.1 39.4 1.0 CB D:ALA216 4.2 24.5 1.0 CB D:THR214 4.3 23.1 1.0 O4P D:G3H1340 4.3 34.3 1.0 C3 D:G3H1340 4.4 35.4 1.0 O2 D:G3H1340 4.4 36.9 1.0 N D:THR154 4.8 14.1 1.0 C2 D:G3H1340 4.8 36.3 1.0 O D:HOH430 4.9 20.1 1.0 CG2 D:THR154 4.9 13.8 1.0 CA D:THR154 5.0 13.9 1.0

W.J.Cook, O.Senkovich, D.Chattopadhyay. An Unexpected Phosphate Binding Site in Glyceraldehyde 3-Phosphate Dehydrogenase: Crystal Structures of Apo, Holo and Ternary Complex of Cryptosporidium Parvum Enzyme Bmc Struct.Biol. V. 9 9 2009.
ISSN: ESSN 1472-6807
PubMed: 19243605
DOI: 10.1186/1472-6807-9-9