Magnesium in PDB 3cu8: Impaired Binding of 14-3-3 to RAF1 Is Linked to Noonan and Leopard Syndrome

All present enzymatic activity of Impaired Binding of 14-3-3 to RAF1 Is Linked to Noonan and Leopard Syndrome:
2.7.11.1;

The structure of Impaired Binding of 14-3-3 to RAF1 Is Linked to Noonan and Leopard Syndrome, PDB code: 3cu8 was solved by B.Schumacher, M.Weyand, J.Kuhlmann, C.Ottmann, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	15.00 / 2.40
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	72.400, 83.840, 111.650, 90.00, 90.00, 90.00
R / Rfree (%)	18.8 / 25.9

The binding sites of Magnesium atom in the Impaired Binding of 14-3-3 to RAF1 Is Linked to Noonan and Leopard Syndrome (pdb code 3cu8). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Impaired Binding of 14-3-3 to RAF1 Is Linked to Noonan and Leopard Syndrome, PDB code: 3cu8:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Impaired Binding of 14-3-3 to RAF1 Is Linked to Noonan and Leopard Syndrome


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Impaired Binding of 14-3-3 to RAF1 Is Linked to Noonan and Leopard Syndrome within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg246 b:88.0 occ:1.00 O3P P:SEP259 2.7 26.4 1.0 NZ A:LYS49 2.9 52.2 1.0 CD A:LYS49 3.1 49.3 1.0 CG A:LYS49 3.2 40.9 1.0 O A:HOH248 3.2 42.7 1.0 CE A:LYS49 3.6 51.3 1.0 O P:HOH117 3.8 45.8 1.0 P P:SEP259 3.8 28.3 1.0 O P:HOH11 3.9 27.4 1.0 O2P P:SEP259 4.0 29.2 1.0 O P:THR260 4.1 33.7 1.0 NH1 A:ARG56 4.1 37.0 1.0 O P:HOH128 4.1 31.8 1.0 OH A:TYR128 4.2 35.2 1.0 OG P:SEP259 4.2 29.4 1.0 CB A:LYS49 4.5 36.9 1.0 O P:HOH99 4.9 37.6 1.0

Magnesium binding site 2 out of 2 in the Impaired Binding of 14-3-3 to RAF1 Is Linked to Noonan and Leopard Syndrome


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Impaired Binding of 14-3-3 to RAF1 Is Linked to Noonan and Leopard Syndrome within 5.0Å range: probe atom residue distance (Å) B Occ Q:Mg1 b:47.9 occ:1.00 O2P Q:SEP259 2.6 29.2 1.0 O Q:THR260 2.7 39.3 1.0 ND2 B:ASN173 2.8 35.4 1.0 N Q:THR260 3.3 38.8 1.0 CE B:LYS49 3.4 63.7 1.0 CB Q:SEP259 3.5 37.5 1.0 OG Q:SEP259 3.5 31.7 1.0 CG B:ASN173 3.6 40.4 1.0 OD1 B:ASN173 3.6 39.4 1.0 C Q:THR260 3.6 39.5 1.0 P Q:SEP259 3.7 33.6 1.0 NZ B:LYS49 3.7 65.0 1.0 CA Q:THR260 3.7 39.1 1.0 CB Q:THR260 3.8 40.0 1.0 C Q:SEP259 3.9 38.4 1.0 OH B:TYR128 4.0 39.7 1.0 O B:HOH266 4.1 64.0 1.0 OD2 B:ASP124 4.2 41.9 1.0 NH2 B:ARG127 4.3 36.9 1.0 CA Q:SEP259 4.3 38.3 1.0 O3P Q:SEP259 4.4 30.2 1.0 OG1 Q:THR260 4.6 41.9 1.0 CG B:ASP124 4.6 38.7 1.0 O Q:SEP259 4.6 38.5 1.0 OD1 B:ASP124 4.7 39.9 1.0 CG2 Q:THR260 4.9 39.5 1.0 CD B:LYS49 4.9 59.0 1.0 N Q:PRO261 4.9 39.7 1.0 CB B:ASN173 5.0 36.8 1.0

M.Molzan, B.Schumacher, C.Ottmann, A.Baljuls, L.Polzien, M.Weyand, P.Thiel, R.Rose, M.Rose, P.Kuhenne, M.Kaiser, U.R.Rapp, J.Kuhlmann, C.Ottmann. Impaired Binding of 14-3-3 to C-Raf in Noonan Syndrome Suggests New Approaches in Diseases with Increased Ras Signaling. Mol. Cell. Biol. V. 30 4698 2010.
ISSN: ESSN 1098-5549
PubMed: 20679480
DOI: 10.1128/MCB.01636-09