Magnesium in PDB 3e2d: The 1.4 A Crystal Structure of the Large and Cold-Active Vibrio Sp. Alkaline Phosphatase

All present enzymatic activity of The 1.4 A Crystal Structure of the Large and Cold-Active Vibrio Sp. Alkaline Phosphatase:
3.1.3.1;

The structure of The 1.4 A Crystal Structure of the Large and Cold-Active Vibrio Sp. Alkaline Phosphatase, PDB code: 3e2d was solved by R.Helland, R.L.Larsen, B.Asgeirsson, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.70 / 1.40
Space group	P 21 21 2
Cell size a, b, c (Å), α, β, γ (°)	118.240, 165.980, 57.480, 90.00, 90.00, 90.00
R / Rfree (%)	15.5 / 16.6

The structure of The 1.4 A Crystal Structure of the Large and Cold-Active Vibrio Sp. Alkaline Phosphatase also contains other interesting chemical elements:

Zinc

(Zn)

4 atoms

The binding sites of Magnesium atom in the The 1.4 A Crystal Structure of the Large and Cold-Active Vibrio Sp. Alkaline Phosphatase (pdb code 3e2d). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the The 1.4 A Crystal Structure of the Large and Cold-Active Vibrio Sp. Alkaline Phosphatase, PDB code: 3e2d:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the The 1.4 A Crystal Structure of the Large and Cold-Active Vibrio Sp. Alkaline Phosphatase


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of The 1.4 A Crystal Structure of the Large and Cold-Active Vibrio Sp. Alkaline Phosphatase within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg603 b:3.0 occ:1.00 OD2 A:ASP12 2.0 3.6 1.0 OE2 A:GLU268 2.0 2.8 1.0 O A:HOH648 2.1 3.2 1.0 O A:HOH629 2.1 3.0 1.0 OG1 A:THR118 2.1 3.7 1.0 O A:HOH693 2.1 3.8 1.0 CG A:ASP12 3.1 3.4 1.0 CD A:GLU268 3.1 2.4 1.0 CB A:THR118 3.2 3.8 1.0 OE1 A:GLU268 3.5 3.5 1.0 OG A:SER65 3.6 7.9 0.5 CB A:ASP12 3.6 2.9 1.0 OG A:SER65 3.8 6.3 0.5 O A:HOH624 4.1 3.6 1.0 ND1 A:HIS116 4.1 6.5 1.0 N A:THR118 4.1 3.4 1.0 OD1 A:ASP12 4.1 3.0 1.0 CG2 A:THR118 4.2 4.3 1.0 CA A:THR118 4.2 3.7 1.0 O A:HOH836 4.3 10.9 1.0 CB A:SER65 4.4 4.7 0.5 CG A:GLU268 4.4 2.7 1.0 CB A:SER65 4.4 4.4 0.5 O1 A:SO4610 4.5 4.7 1.0 O A:GLY270 4.5 4.3 1.0 CA A:GLY270 4.6 3.5 1.0 CE1 A:HIS116 4.7 6.9 1.0 ZN A:ZN602 4.7 4.8 1.0 OD2 A:ASP315 4.8 3.7 1.0 OG1 A:THR112 4.9 4.1 1.0

Magnesium binding site 2 out of 2 in the The 1.4 A Crystal Structure of the Large and Cold-Active Vibrio Sp. Alkaline Phosphatase


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of The 1.4 A Crystal Structure of the Large and Cold-Active Vibrio Sp. Alkaline Phosphatase within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg603 b:3.2 occ:1.00 OE2 B:GLU268 2.0 3.3 1.0 OD2 B:ASP12 2.0 3.4 1.0 O B:HOH937 2.1 3.8 1.0 O B:HOH947 2.1 4.2 1.0 O B:HOH923 2.1 4.8 1.0 OG1 B:THR118 2.2 3.5 1.0 CD B:GLU268 3.1 3.4 1.0 CG B:ASP12 3.1 2.8 1.0 CB B:THR118 3.2 3.2 1.0 OE1 B:GLU268 3.4 4.1 1.0 OG B:SER65 3.6 8.6 0.5 CB B:ASP12 3.6 3.5 1.0 OG B:SER65 3.9 6.8 0.5 ND1 B:HIS116 4.1 7.0 1.0 O B:HOH921 4.1 3.9 1.0 OD1 B:ASP12 4.1 2.2 1.0 N B:THR118 4.1 3.7 1.0 CG2 B:THR118 4.2 4.7 1.0 CA B:THR118 4.3 3.5 1.0 O B:HOH1326 4.3 12.0 1.0 CG B:GLU268 4.4 3.6 1.0 CB B:SER65 4.4 5.3 0.5 CB B:SER65 4.4 5.0 0.5 O1 B:SO4610 4.5 6.7 1.0 O B:GLY270 4.5 4.2 1.0 CA B:GLY270 4.6 3.6 1.0 CE1 B:HIS116 4.7 7.8 1.0 ZN B:ZN602 4.7 5.0 1.0 OD2 B:ASP315 4.8 4.0 1.0 OG1 B:THR112 4.9 5.0 1.0

R.Helland, R.L.Larsen, B.Asgeirsson. The 1.4 A Crystal Structure of the Large and Cold-Active Vibrio Sp. Alkaline Phosphatase. Biochim.Biophys.Acta V.1794 297 2009.
ISSN: ISSN 0006-3002
PubMed: 18977465
DOI: 10.1016/J.BBAPAP.2008.09.020