Magnesium in PDB 3g2c: MTH0212 in Complex with A Short Ssdna (Cgta)

All present enzymatic activity of MTH0212 in Complex with A Short Ssdna (Cgta):
3.1.11.2;

The structure of MTH0212 in Complex with A Short Ssdna (Cgta), PDB code: 3g2c was solved by K.Lakomek, A.Dickmanns, R.Ficner, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	28.27 / 2.30
Space group	P 32
Cell size a, b, c (Å), α, β, γ (°)	80.325, 80.325, 79.611, 90.00, 90.00, 120.00
R / Rfree (%)	20.4 / 26.3

The binding sites of Magnesium atom in the MTH0212 in Complex with A Short Ssdna (Cgta) (pdb code 3g2c). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the MTH0212 in Complex with A Short Ssdna (Cgta), PDB code: 3g2c:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the MTH0212 in Complex with A Short Ssdna (Cgta)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of MTH0212 in Complex with A Short Ssdna (Cgta) within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg271 b:49.1 occ:1.00 OE2 A:GLU38 2.6 38.7 1.0 O3 A:PO4272 2.7 46.4 1.0 OE1 A:GLU38 2.7 39.4 1.0 OD1 A:ASN12 2.9 32.1 1.0 CD A:GLU38 3.0 36.0 1.0 CG A:ASN12 3.6 31.3 1.0 OD2 A:ASP247 4.0 29.8 1.0 P A:PO4272 4.1 49.5 1.0 CB A:ASN12 4.2 29.3 1.0 O2 A:PO4272 4.3 50.5 1.0 OD1 A:ASP247 4.3 33.5 1.0 CA A:ASN12 4.5 28.5 1.0 ND2 A:ASN12 4.5 34.4 1.0 CG A:GLU38 4.5 31.3 1.0 OD1 A:ASN10 4.5 22.9 1.0 OH A:TYR111 4.6 30.9 1.0 ND2 A:ASN10 4.6 18.8 1.0 CG A:ASP247 4.6 29.0 1.0 NZ A:LYS40 4.6 38.9 1.0 O4 A:PO4272 4.7 47.3 1.0 CE A:LYS40 4.9 38.3 1.0 CG A:ASN10 5.0 22.0 1.0

Magnesium binding site 2 out of 2 in the MTH0212 in Complex with A Short Ssdna (Cgta)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of MTH0212 in Complex with A Short Ssdna (Cgta) within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg266 b:48.3 occ:1.00 OE2 B:GLU38 2.1 40.6 1.0 O1 B:PO4267 2.2 47.4 1.0 OD1 B:ASN12 2.7 42.0 1.0 CD B:GLU38 3.1 41.5 1.0 CG B:ASN12 3.4 43.0 1.0 P B:PO4267 3.6 50.1 1.0 OE1 B:GLU38 3.6 41.4 1.0 OD1 B:ASP247 3.8 37.9 1.0 OD1 B:ASN10 3.8 35.9 1.0 CB B:ASN12 3.9 42.1 1.0 OD2 B:ASP247 4.0 39.4 1.0 O4 B:PO4267 4.0 51.9 1.0 CA B:ASN12 4.1 42.1 1.0 ND2 B:ASN10 4.2 33.8 1.0 O3 B:PO4267 4.2 49.7 1.0 OH B:TYR111 4.3 29.6 1.0 CG B:ASP247 4.3 39.2 1.0 ND2 B:ASN12 4.3 43.2 1.0 CG B:ASN10 4.4 35.6 1.0 CG B:GLU38 4.4 38.1 1.0 CD2 B:HIS248 4.5 36.0 1.0 NE2 B:HIS248 4.6 37.5 1.0 O2 B:PO4267 4.7 51.0 1.0 N B:ASN12 4.8 41.3 1.0 CB B:GLU38 4.9 36.1 1.0

K.Lakomek, A.Dickmanns, E.Ciirdaeva, L.Schomacher, R.Ficner. Crystal Structure Analysis of Dna Uridine Endonuclease MTH212 Bound to Dna J.Mol.Biol. V. 399 604 2010.
ISSN: ISSN 0022-2836
PubMed: 20434457
DOI: 10.1016/J.JMB.2010.04.044