Magnesium in PDB 3g4t: MTH0212 (Wt) in Complex with A 7BP Dsdna

All present enzymatic activity of MTH0212 (Wt) in Complex with A 7BP Dsdna:
3.1.11.2;

The structure of MTH0212 (Wt) in Complex with A 7BP Dsdna, PDB code: 3g4t was solved by K.Lakomek, A.Dickmanns, R.Ficner, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	42.57 / 2.64
Space group	P 21 21 2
Cell size a, b, c (Å), α, β, γ (°)	100.764, 79.600, 99.409, 90.00, 90.00, 90.00
R / Rfree (%)	24 / 29.7

The binding sites of Magnesium atom in the MTH0212 (Wt) in Complex with A 7BP Dsdna (pdb code 3g4t). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the MTH0212 (Wt) in Complex with A 7BP Dsdna, PDB code: 3g4t:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the MTH0212 (Wt) in Complex with A 7BP Dsdna


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of MTH0212 (Wt) in Complex with A 7BP Dsdna within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg267 b:77.2 occ:1.00 O4 A:PO4266 2.3 64.6 1.0 OE2 A:GLU38 2.4 56.1 1.0 O3' H:DA7 2.6 69.4 1.0 OD2 A:ASP247 3.2 52.4 1.0 OD1 A:ASP247 3.3 56.0 1.0 CD A:GLU38 3.4 55.9 1.0 NE2 A:HIS248 3.6 51.0 1.0 CG A:ASP247 3.7 54.8 1.0 OD1 A:ASN12 3.7 46.6 1.0 OD1 A:ASN10 3.7 49.8 1.0 P A:PO4266 3.8 63.8 1.0 CD2 A:HIS248 3.8 51.2 1.0 OE1 A:GLU38 3.8 58.6 1.0 ND2 A:ASN10 3.9 46.9 1.0 CG A:ASN12 4.0 48.8 1.0 C3' H:DA7 4.0 69.8 1.0 CG A:ASN10 4.1 48.9 1.0 O3 A:PO4266 4.4 59.3 1.0 CB A:ASN12 4.4 49.7 1.0 O1 A:PO4266 4.4 66.2 1.0 CG A:GLU38 4.6 52.6 1.0 ND2 A:ASN12 4.7 49.4 1.0 O2 A:PO4266 4.7 63.3 1.0 C2' H:DA7 4.7 70.2 1.0 CA A:ASN12 4.9 49.5 1.0 CE1 A:HIS248 4.9 50.6 1.0 C4' H:DA7 4.9 69.5 1.0 NE1 A:TRP205 4.9 67.5 1.0

Magnesium binding site 2 out of 2 in the MTH0212 (Wt) in Complex with A 7BP Dsdna


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of MTH0212 (Wt) in Complex with A 7BP Dsdna within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg267 b:80.7 occ:1.00 O4 B:PO4266 2.3 76.1 1.0 OE2 B:GLU38 2.8 56.4 1.0 O3' I:DG9 2.8 72.7 1.0 OD1 B:ASP247 3.1 57.9 1.0 OD2 B:ASP247 3.2 53.5 1.0 CG B:ASP247 3.5 56.4 1.0 NE2 B:HIS248 3.6 53.4 1.0 OD1 B:ASN10 3.6 51.2 1.0 CD2 B:HIS248 3.7 53.4 1.0 CD B:GLU38 3.7 56.8 1.0 OD1 B:ASN12 3.7 47.7 1.0 P B:PO4266 3.8 76.5 1.0 CG B:ASN12 3.9 49.9 1.0 ND2 B:ASN10 4.1 47.9 1.0 OE1 B:GLU38 4.1 59.7 1.0 CG B:ASN10 4.1 50.1 1.0 CB B:ASN12 4.2 50.5 1.0 C3' I:DG9 4.2 73.8 1.0 O3 B:PO4266 4.3 78.5 1.0 O1 B:PO4266 4.5 74.5 1.0 ND2 B:ASN12 4.5 49.8 1.0 O2 B:PO4266 4.7 75.6 1.0 CA B:ASN12 4.8 50.5 1.0 CG B:GLU38 4.9 54.0 1.0 CE1 B:HIS248 4.9 52.5 1.0 NE1 B:TRP205 4.9 77.0 1.0 C2' I:DG9 5.0 73.5 1.0

K.Lakomek, A.Dickmanns, E.Ciirdaeva, L.Schomacher, R.Ficner. Crystal Structure Analysis of Dna Uridine Endonuclease MTH212 Bound to Dna J.Mol.Biol. V. 399 604 2010.
ISSN: ISSN 0022-2836
PubMed: 20434457
DOI: 10.1016/J.JMB.2010.04.044