Atomistry » Magnesium » PDB 3fzn-3g8c » 3g6w
Atomistry »
  Magnesium »
    PDB 3fzn-3g8c »
      3g6w »

Magnesium in PDB 3g6w: Asymetric Gtp Bound Structure of Uprtase From Sulfolobus Solfataricus Containing Prpp-MG2+ in Half of the Active Sites and R5P and Ppi in the Other Half

Enzymatic activity of Asymetric Gtp Bound Structure of Uprtase From Sulfolobus Solfataricus Containing Prpp-MG2+ in Half of the Active Sites and R5P and Ppi in the Other Half

All present enzymatic activity of Asymetric Gtp Bound Structure of Uprtase From Sulfolobus Solfataricus Containing Prpp-MG2+ in Half of the Active Sites and R5P and Ppi in the Other Half:
2.4.2.9;

Protein crystallography data

The structure of Asymetric Gtp Bound Structure of Uprtase From Sulfolobus Solfataricus Containing Prpp-MG2+ in Half of the Active Sites and R5P and Ppi in the Other Half, PDB code: 3g6w was solved by A.Kadziola, S.Christoffersen, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 25.00 / 2.90
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 60.038, 98.758, 73.528, 90.00, 92.80, 90.00
R / Rfree (%) 21.6 / 27.4

Magnesium Binding Sites:

The binding sites of Magnesium atom in the Asymetric Gtp Bound Structure of Uprtase From Sulfolobus Solfataricus Containing Prpp-MG2+ in Half of the Active Sites and R5P and Ppi in the Other Half (pdb code 3g6w). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 4 binding sites of Magnesium where determined in the Asymetric Gtp Bound Structure of Uprtase From Sulfolobus Solfataricus Containing Prpp-MG2+ in Half of the Active Sites and R5P and Ppi in the Other Half, PDB code: 3g6w:
Jump to Magnesium binding site number: 1; 2; 3; 4;

Magnesium binding site 1 out of 4 in 3g6w

Go back to Magnesium Binding Sites List in 3g6w
Magnesium binding site 1 out of 4 in the Asymetric Gtp Bound Structure of Uprtase From Sulfolobus Solfataricus Containing Prpp-MG2+ in Half of the Active Sites and R5P and Ppi in the Other Half


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Asymetric Gtp Bound Structure of Uprtase From Sulfolobus Solfataricus Containing Prpp-MG2+ in Half of the Active Sites and R5P and Ppi in the Other Half within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg302

b:92.1
occ:1.00
 O1B A:GTP301 1.9 30.5 1.0
O1A D:GTP217 2.0 24.1 1.0
O1A A:GTP301 2.0 28.1 1.0
O A:HOH229 2.1 56.6 1.0
O1B D:GTP217 2.1 26.8 1.0
O A:HOH219 2.6 29.5 1.0
PB A:GTP301 3.1 27.8 1.0
PA A:GTP301 3.2 28.8 1.0
PA D:GTP217 3.3 27.7 1.0
PB D:GTP217 3.4 25.7 1.0
O3A A:GTP301 3.4 29.4 1.0
O3A D:GTP217 3.6 25.4 1.0
O2G A:GTP301 3.7 35.6 1.0
O A:HOH221 3.8 21.8 1.0
O5' A:GTP301 3.9 29.5 1.0
O2G D:GTP217 4.0 29.5 1.0
O2B A:GTP301 4.1 31.4 1.0
O5' D:GTP217 4.1 29.9 1.0
O3B A:GTP301 4.2 33.0 1.0
O2B D:GTP217 4.2 28.5 1.0
CE A:LYS30 4.4 28.9 1.0
O2A A:GTP301 4.4 30.1 1.0
PG A:GTP301 4.4 36.1 1.0
O2A D:GTP217 4.4 24.9 1.0
NZ A:LYS30 4.5 25.7 1.0
O3B D:GTP217 4.5 29.4 1.0
N7 D:GTP217 4.6 23.6 1.0
O3G A:GTP301 4.6 35.5 1.0
C8 D:GTP217 4.6 22.9 1.0
CE D:LYS30 4.7 28.4 1.0
C8 A:GTP301 4.7 29.1 1.0
PG D:GTP217 4.8 31.0 1.0
NZ D:LYS30 4.9 27.5 1.0
N7 A:GTP301 4.9 28.3 1.0

Magnesium binding site 2 out of 4 in 3g6w

Go back to Magnesium Binding Sites List in 3g6w
Magnesium binding site 2 out of 4 in the Asymetric Gtp Bound Structure of Uprtase From Sulfolobus Solfataricus Containing Prpp-MG2+ in Half of the Active Sites and R5P and Ppi in the Other Half


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Asymetric Gtp Bound Structure of Uprtase From Sulfolobus Solfataricus Containing Prpp-MG2+ in Half of the Active Sites and R5P and Ppi in the Other Half within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg305

b:65.5
occ:1.00
 O A:HOH218 2.2 40.2 1.0
O2B A:PRP303 2.2 71.3 1.0
O A:HOH217 2.2 42.9 1.0
O3A A:PRP303 2.3 71.6 1.0
PB A:PRP303 2.3 72.2 1.0
O1B A:PRP303 2.3 70.0 1.0
O1 A:PRP303 2.8 69.0 1.0
PA A:PRP303 3.2 69.6 1.0
O A:LEU79 3.5 35.4 1.0
O2 A:PRP303 3.5 66.5 1.0
O3 A:PRP303 3.5 63.8 1.0
CB A:ARG80 3.6 33.1 1.0
O3B A:PRP303 3.8 71.5 1.0
N A:ALA81 4.0 31.2 1.0
O1A A:PRP303 4.0 68.8 1.0
C1 A:PRP303 4.1 67.2 1.0
C2 A:PRP303 4.2 66.5 1.0
CB A:ALA81 4.3 31.0 1.0
O2A A:PRP303 4.3 69.0 1.0
C3 A:PRP303 4.5 65.2 1.0
C A:LEU79 4.6 36.4 1.0
CG A:ARG80 4.6 33.2 1.0
CA A:ARG80 4.7 33.8 1.0
CA A:ALA81 4.8 30.9 1.0
C A:ARG80 4.8 32.6 1.0
NE A:ARG80 5.0 36.1 1.0

Magnesium binding site 3 out of 4 in 3g6w

Go back to Magnesium Binding Sites List in 3g6w
Magnesium binding site 3 out of 4 in the Asymetric Gtp Bound Structure of Uprtase From Sulfolobus Solfataricus Containing Prpp-MG2+ in Half of the Active Sites and R5P and Ppi in the Other Half


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Asymetric Gtp Bound Structure of Uprtase From Sulfolobus Solfataricus Containing Prpp-MG2+ in Half of the Active Sites and R5P and Ppi in the Other Half within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Mg302

b:35.0
occ:1.00
 O D:HOH218 1.9 28.2 1.0
O1A B:GTP301 2.0 35.5 1.0
O1B D:GTP301 2.0 40.9 1.0
O1A D:GTP301 2.1 32.9 1.0
O B:HOH218 2.1 22.6 1.0
O1B B:GTP301 2.1 38.7 1.0
PA D:GTP301 3.3 36.1 1.0
PB D:GTP301 3.3 37.1 1.0
PA B:GTP301 3.3 38.6 1.0
PB B:GTP301 3.4 35.5 1.0
O3A D:GTP301 3.5 36.7 1.0
O2G B:GTP301 3.6 41.2 1.0
O3A B:GTP301 3.6 37.2 1.0
O5' D:GTP301 3.9 35.2 1.0
O3G D:GTP301 3.9 39.5 1.0
O5' B:GTP301 4.0 38.8 1.0
O2B D:GTP301 4.2 38.6 1.0
C8 D:GTP301 4.3 41.1 1.0
O3B D:GTP301 4.3 38.8 1.0
N7 D:GTP301 4.3 41.6 1.0
O2B B:GTP301 4.3 41.4 1.0
O3B B:GTP301 4.4 39.4 1.0
C8 B:GTP301 4.5 39.0 1.0
O2A B:GTP301 4.5 39.5 1.0
CE B:LYS30 4.5 29.3 1.0
O2A D:GTP301 4.5 35.5 1.0
N7 B:GTP301 4.5 38.9 1.0
PG D:GTP301 4.5 39.5 1.0
O2G D:GTP301 4.6 39.2 1.0
NZ B:LYS30 4.6 27.7 1.0
PG B:GTP301 4.6 42.7 1.0
CE C:LYS30 4.8 30.1 1.0
NZ C:LYS30 5.0 28.9 1.0

Magnesium binding site 4 out of 4 in 3g6w

Go back to Magnesium Binding Sites List in 3g6w
Magnesium binding site 4 out of 4 in the Asymetric Gtp Bound Structure of Uprtase From Sulfolobus Solfataricus Containing Prpp-MG2+ in Half of the Active Sites and R5P and Ppi in the Other Half


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 4 of Asymetric Gtp Bound Structure of Uprtase From Sulfolobus Solfataricus Containing Prpp-MG2+ in Half of the Active Sites and R5P and Ppi in the Other Half within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Mg304

b:80.6
occ:1.00
 O1B D:PRP303 1.9 66.0 1.0
O D:HOH220 2.0 42.1 1.0
O D:HOH219 2.1 38.5 1.0
O3B D:PRP303 2.2 63.2 1.0
PB D:PRP303 2.2 66.0 1.0
O3A D:PRP303 2.5 65.3 1.0
O1 D:PRP303 2.6 64.3 1.0
O2 D:PRP303 2.9 59.0 1.0
O D:LEU79 3.2 26.4 1.0
PA D:PRP303 3.3 65.7 1.0
N D:ALA81 3.6 27.5 1.0
CB D:ARG80 3.6 28.0 1.0
C1 D:PRP303 3.7 61.6 1.0
CB D:ALA81 3.7 29.5 1.0
O2B D:PRP303 3.7 66.4 1.0
C2 D:PRP303 3.9 59.4 1.0
C D:LEU79 4.2 27.4 1.0
O1A D:PRP303 4.2 67.7 1.0
O3 D:PRP303 4.2 57.8 1.0
CA D:ALA81 4.3 26.6 1.0
O2A D:PRP303 4.3 65.8 1.0
CA D:ARG80 4.5 28.5 1.0
C D:ARG80 4.5 28.3 1.0
CG D:ARG80 4.7 29.1 1.0
N D:ARG80 4.7 30.0 1.0
C3 D:PRP303 4.7 57.6 1.0
O D:ALA103 4.8 53.2 1.0
NH1 D:ARG80 4.8 28.6 1.0
O4 D:PRP303 4.9 60.0 1.0

Reference:

S.Christoffersen, A.Kadziola, E.Johansson, M.Rasmussen, M.Willemoes, K.F.Jensen. Structural and Kinetic Studies of the Allosteric Transition in Sulfolobus Solfataricus Uracil Phosphoribosyltransferase: Permanent Activation By Engineering of the C-Terminus J.Mol.Biol. V. 393 464 2009.
ISSN: ISSN 0022-2836
PubMed: 19683539
DOI: 10.1016/J.JMB.2009.08.019
Page generated: Wed Aug 14 14:08:31 2024

Last articles

Zn in 9JYW
Zn in 9IR4
Zn in 9IR3
Zn in 9GMX
Zn in 9GMW
Zn in 9JEJ
Zn in 9ERF
Zn in 9ERE
Zn in 9EGV
Zn in 9EGW
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy