Magnesium in the structure of Crystal Stucture of Biotin Carboxylase in Complex With Biotin, Bicarbonate, Adp and Mg Ion (pdb 3g8c)

The binding sites of Magnesium atom in the structure of Crystal Stucture of Biotin Carboxylase in Complex With Biotin, Bicarbonate, Adp and Mg Ion (pdb code 3g8c). This binding sites where shown with 5.0 Angstroms radius around Magnesium atom. The 3g8c structure was solved by C.Y.CHOU, L.P.YU, L.TONG, with X-Ray Crystallography technique, brief refinement statistics is given in the table below: Resolution (A) 30.0-2.0 Space group P212121 a (A) 83.317 b (A) 106.166 c (A) 121.486 alpha (°) 90.00 beta (°) 90.00 gamma (°) 90.00 Rfactor (%) 18.2 Rfree (%) 21.6 Magnesium Binding Sites: Magnesium binding site 1 out of 2 in 3g8c Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 1 of Magnesium in the PDB 3g8c. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Magnesium atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: A: Glu276, A: Glu288, A: Asn290, A: Adp1000, A: Hoh538, A: Hoh592, A: Hoh640, A: Hoh717, A: Hoh756, A: Hoh777, conact list: Atom Atom Distance (A) Mg OE1 A:Glu276 2.32 Mg OE2 A:Glu276 3.61 Mg CD A:Glu276 3.31 Mg CG A:Glu276 4.63 Mg OE1 A:Glu288 2.13 Mg OE2 A:Glu288 4.30 Mg CD A:Glu288 3.19 Mg CG A:Glu288 3.61 Mg ND2 A:Asn290 4.63 Mg OD1 A:Asn290 4.11 Mg CG A:Asn290 4.81 Mg O3B A:Adp1000 3.83 Mg PA A:Adp1000 3.43 Mg O2A A:Adp1000 2.11 Mg O1B A:Adp1000 2.19 Mg PB A:Adp1000 3.38 Mg C5' A:Adp1000 4.16 Mg O5' A:Adp1000 4.29 Mg O2B A:Adp1000 4.69 Mg O3A A:Adp1000 3.79 Mg O1A A:Adp1000 4.59 Mg O A:Hoh538 1.96 Mg O A:Hoh592 3.65 Mg O A:Hoh640 2.13 Mg O A:Hoh717 4.28 Mg O A:Hoh756 4.00 Mg O A:Hoh777 4.57 interactive model: Magnesium binding site 2 out of 2 in 3g8c Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 2 of Magnesium in the PDB 3g8c. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Magnesium atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: B: Glu276, B: Glu288, B: Asn290, B: Adp1001, B: Hoh503, B: Hoh575, B: Hoh753, B: Hoh804, B: Hoh841, conact list: Atom Atom Distance (A) Mg OE1 B:Glu276 2.47 Mg OE2 B:Glu276 3.63 Mg CD B:Glu276 3.39 Mg CG B:Glu276 4.73 Mg OE1 B:Glu288 4.27 Mg OE2 B:Glu288 2.07 Mg CD B:Glu288 3.20 Mg CG B:Glu288 3.71 Mg ND2 B:Asn290 4.67 Mg OD1 B:Asn290 4.15 Mg CG B:Asn290 4.85 Mg O3B B:Adp1001 3.84 Mg PA B:Adp1001 3.88 Mg O2A B:Adp1001 2.60 Mg O1B B:Adp1001 2.26 Mg PB B:Adp1001 3.50 Mg C5' B:Adp1001 4.68 Mg O5' B:Adp1001 4.67 Mg O2B B:Adp1001 4.76 Mg O3A B:Adp1001 4.11 Mg O B:Hoh503 3.50 Mg O B:Hoh575 4.60 Mg O B:Hoh753 1.97 Mg O B:Hoh804 2.16 Mg O B:Hoh841 3.74 interactive model: