Magnesium in PDB 3glg: Crystal Structure of A Mutant (GAMMAT157A) E. Coli Clamp Loader Bound to Primer-Template Dna
Enzymatic activity of Crystal Structure of A Mutant (GAMMAT157A) E. Coli Clamp Loader Bound to Primer-Template Dna
All present enzymatic activity of Crystal Structure of A Mutant (GAMMAT157A) E. Coli Clamp Loader Bound to Primer-Template Dna:
2.7.7.7;
Protein crystallography data
The structure of Crystal Structure of A Mutant (GAMMAT157A) E. Coli Clamp Loader Bound to Primer-Template Dna, PDB code: 3glg
was solved by
K.R.Simonetta,
S.N.Seyedin,
J.Kuriyan,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
49.24 /
3.25
|
Space group
|
P 21 21 21
|
Cell size a, b, c (Å), α, β, γ (°)
|
100.102,
219.104,
274.664,
90.00,
90.00,
90.00
|
R / Rfree (%)
|
22.4 /
26.3
|
Other elements in 3glg:
The structure of Crystal Structure of A Mutant (GAMMAT157A) E. Coli Clamp Loader Bound to Primer-Template Dna also contains other interesting chemical elements:
Magnesium Binding Sites:
The binding sites of Magnesium atom in the Crystal Structure of A Mutant (GAMMAT157A) E. Coli Clamp Loader Bound to Primer-Template Dna
(pdb code 3glg). This binding sites where shown within
5.0 Angstroms radius around Magnesium atom.
In total 6 binding sites of Magnesium where determined in the
Crystal Structure of A Mutant (GAMMAT157A) E. Coli Clamp Loader Bound to Primer-Template Dna, PDB code: 3glg:
Jump to Magnesium binding site number:
1;
2;
3;
4;
5;
6;
Magnesium binding site 1 out
of 6 in 3glg
Go back to
Magnesium Binding Sites List in 3glg
Magnesium binding site 1 out
of 6 in the Crystal Structure of A Mutant (GAMMAT157A) E. Coli Clamp Loader Bound to Primer-Template Dna
Mono view
Stereo pair view
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 1 of Crystal Structure of A Mutant (GAMMAT157A) E. Coli Clamp Loader Bound to Primer-Template Dna within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Mg412
b:89.5
occ:1.00
|
F1
|
B:BEF401
|
2.1
|
95.3
|
1.0
|
O1B
|
B:ADP400
|
2.1
|
0.7
|
1.0
|
CG2
|
B:THR52
|
2.3
|
0.4
|
1.0
|
OD1
|
B:ASP126
|
3.3
|
87.4
|
1.0
|
OD2
|
B:ASP126
|
3.3
|
88.4
|
1.0
|
BE
|
B:BEF401
|
3.3
|
95.4
|
1.0
|
PB
|
B:ADP400
|
3.5
|
0.5
|
1.0
|
OE2
|
B:GLU127
|
3.6
|
90.2
|
1.0
|
CB
|
B:THR52
|
3.6
|
1.0
|
1.0
|
F3
|
B:BEF401
|
3.6
|
94.6
|
1.0
|
CG
|
B:ASP126
|
3.7
|
87.8
|
1.0
|
OG1
|
B:THR52
|
3.7
|
0.5
|
1.0
|
O3B
|
B:ADP400
|
3.9
|
0.7
|
1.0
|
OE2
|
C:GLU144
|
4.2
|
0.3
|
1.0
|
NH2
|
C:ARG169
|
4.2
|
85.3
|
1.0
|
O2A
|
B:ADP400
|
4.3
|
0.1
|
1.0
|
N
|
B:THR52
|
4.5
|
1.0
|
1.0
|
O2B
|
B:ADP400
|
4.5
|
0.1
|
1.0
|
CD
|
B:GLU127
|
4.5
|
89.6
|
1.0
|
O3A
|
B:ADP400
|
4.6
|
1.0
|
1.0
|
F2
|
B:BEF401
|
4.6
|
95.6
|
1.0
|
CA
|
B:THR52
|
4.7
|
0.2
|
1.0
|
CG
|
B:GLU127
|
4.7
|
89.5
|
1.0
|
NZ
|
C:LYS141
|
5.0
|
90.1
|
1.0
|
|
Magnesium binding site 2 out
of 6 in 3glg
Go back to
Magnesium Binding Sites List in 3glg
Magnesium binding site 2 out
of 6 in the Crystal Structure of A Mutant (GAMMAT157A) E. Coli Clamp Loader Bound to Primer-Template Dna
Mono view
Stereo pair view
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 2 of Crystal Structure of A Mutant (GAMMAT157A) E. Coli Clamp Loader Bound to Primer-Template Dna within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
C:Mg413
b:89.0
occ:1.00
|
OG1
|
C:THR52
|
2.0
|
90.2
|
1.0
|
F1
|
C:BEF403
|
2.1
|
95.9
|
1.0
|
O1B
|
C:ADP402
|
2.1
|
90.3
|
1.0
|
OE2
|
C:GLU127
|
3.1
|
92.8
|
1.0
|
OD1
|
C:ASP126
|
3.1
|
97.6
|
1.0
|
CB
|
C:THR52
|
3.2
|
91.6
|
1.0
|
BE
|
C:BEF403
|
3.5
|
96.8
|
1.0
|
PB
|
C:ADP402
|
3.5
|
91.3
|
1.0
|
O2A
|
C:ADP402
|
3.7
|
92.8
|
1.0
|
NH2
|
D:ARG169
|
3.8
|
91.7
|
1.0
|
CG2
|
C:THR52
|
3.9
|
91.7
|
1.0
|
O3B
|
C:ADP402
|
4.0
|
90.8
|
1.0
|
CG
|
C:ASP126
|
4.0
|
96.6
|
1.0
|
F2
|
C:BEF403
|
4.0
|
97.7
|
1.0
|
OD2
|
C:ASP126
|
4.1
|
96.4
|
1.0
|
OE2
|
D:GLU144
|
4.2
|
0.1
|
1.0
|
CD
|
C:GLU127
|
4.3
|
92.0
|
1.0
|
CA
|
C:THR52
|
4.4
|
92.2
|
1.0
|
N
|
C:THR52
|
4.5
|
92.2
|
1.0
|
O3A
|
C:ADP402
|
4.5
|
93.1
|
1.0
|
OE1
|
D:GLU144
|
4.5
|
0.8
|
1.0
|
F3
|
C:BEF403
|
4.6
|
96.1
|
1.0
|
O2B
|
C:ADP402
|
4.7
|
91.8
|
1.0
|
NH2
|
C:ARG215
|
4.7
|
99.4
|
1.0
|
PA
|
C:ADP402
|
4.7
|
94.0
|
1.0
|
CD
|
D:GLU144
|
4.8
|
0.5
|
1.0
|
OE1
|
C:GLU127
|
4.9
|
91.8
|
1.0
|
CD
|
D:LYS141
|
5.0
|
97.4
|
1.0
|
|
Magnesium binding site 3 out
of 6 in 3glg
Go back to
Magnesium Binding Sites List in 3glg
Magnesium binding site 3 out
of 6 in the Crystal Structure of A Mutant (GAMMAT157A) E. Coli Clamp Loader Bound to Primer-Template Dna
Mono view
Stereo pair view
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 3 of Crystal Structure of A Mutant (GAMMAT157A) E. Coli Clamp Loader Bound to Primer-Template Dna within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
D:Mg414
b:85.8
occ:1.00
|
F1
|
D:BEF405
|
2.1
|
1.0
|
1.0
|
O1B
|
D:ADP404
|
2.1
|
93.2
|
1.0
|
CG2
|
D:THR52
|
2.3
|
89.3
|
1.0
|
OD2
|
D:ASP126
|
2.9
|
97.9
|
1.0
|
OE2
|
D:GLU127
|
3.3
|
85.1
|
1.0
|
CB
|
D:THR52
|
3.4
|
91.2
|
1.0
|
BE
|
D:BEF405
|
3.5
|
0.7
|
1.0
|
OG1
|
D:THR52
|
3.5
|
92.3
|
1.0
|
PB
|
D:ADP404
|
3.5
|
93.8
|
1.0
|
OD1
|
D:ASP126
|
3.7
|
96.4
|
1.0
|
CG
|
D:ASP126
|
3.7
|
97.0
|
1.0
|
NH2
|
E:ARG158
|
3.9
|
87.7
|
1.0
|
O2A
|
D:ADP404
|
3.9
|
96.5
|
1.0
|
O3B
|
D:ADP404
|
4.0
|
92.8
|
1.0
|
F2
|
D:BEF405
|
4.1
|
0.2
|
1.0
|
OE1
|
E:GLU133
|
4.4
|
0.2
|
1.0
|
N
|
D:THR52
|
4.4
|
92.1
|
1.0
|
NZ
|
E:LYS130
|
4.5
|
0.6
|
1.0
|
O2B
|
D:ADP404
|
4.5
|
93.7
|
1.0
|
CD
|
D:GLU127
|
4.6
|
84.0
|
1.0
|
CA
|
D:THR52
|
4.6
|
92.5
|
1.0
|
O3A
|
D:ADP404
|
4.7
|
96.1
|
1.0
|
NH2
|
D:ARG215
|
4.7
|
95.8
|
1.0
|
F3
|
D:BEF405
|
4.7
|
0.7
|
1.0
|
PA
|
D:ADP404
|
4.9
|
97.7
|
1.0
|
|
Magnesium binding site 4 out
of 6 in 3glg
Go back to
Magnesium Binding Sites List in 3glg
Magnesium binding site 4 out
of 6 in the Crystal Structure of A Mutant (GAMMAT157A) E. Coli Clamp Loader Bound to Primer-Template Dna
Mono view
Stereo pair view
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 4 of Crystal Structure of A Mutant (GAMMAT157A) E. Coli Clamp Loader Bound to Primer-Template Dna within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
G:Mg415
b:89.5
occ:1.00
|
F1
|
G:BEF407
|
2.1
|
97.8
|
1.0
|
O1B
|
G:ADP406
|
2.1
|
93.2
|
1.0
|
CG2
|
G:THR52
|
2.3
|
94.0
|
1.0
|
BE
|
G:BEF407
|
3.3
|
97.1
|
1.0
|
OD1
|
G:ASP126
|
3.4
|
93.5
|
1.0
|
PB
|
G:ADP406
|
3.4
|
93.1
|
1.0
|
OD2
|
G:ASP126
|
3.5
|
94.9
|
1.0
|
OE2
|
G:GLU127
|
3.5
|
95.1
|
1.0
|
CB
|
G:THR52
|
3.6
|
95.8
|
1.0
|
F3
|
G:BEF407
|
3.6
|
96.7
|
1.0
|
CG
|
G:ASP126
|
3.8
|
94.2
|
1.0
|
O3B
|
G:ADP406
|
3.8
|
91.6
|
1.0
|
OG1
|
G:THR52
|
3.8
|
96.2
|
1.0
|
OE2
|
H:GLU144
|
4.2
|
0.2
|
1.0
|
NH2
|
H:ARG169
|
4.2
|
96.4
|
1.0
|
O2A
|
G:ADP406
|
4.2
|
95.2
|
1.0
|
CD
|
G:GLU127
|
4.4
|
94.8
|
1.0
|
O2B
|
G:ADP406
|
4.5
|
93.5
|
1.0
|
N
|
G:THR52
|
4.5
|
96.1
|
1.0
|
O3A
|
G:ADP406
|
4.6
|
94.0
|
1.0
|
F2
|
G:BEF407
|
4.6
|
96.5
|
1.0
|
CA
|
G:THR52
|
4.7
|
96.9
|
1.0
|
CG
|
G:GLU127
|
4.7
|
95.2
|
1.0
|
NZ
|
H:LYS141
|
4.8
|
89.0
|
1.0
|
CE
|
G:LYS51
|
4.9
|
91.2
|
1.0
|
|
Magnesium binding site 5 out
of 6 in 3glg
Go back to
Magnesium Binding Sites List in 3glg
Magnesium binding site 5 out
of 6 in the Crystal Structure of A Mutant (GAMMAT157A) E. Coli Clamp Loader Bound to Primer-Template Dna
Mono view
Stereo pair view
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 5 of Crystal Structure of A Mutant (GAMMAT157A) E. Coli Clamp Loader Bound to Primer-Template Dna within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
H:Mg416
b:63.9
occ:1.00
|
OG1
|
H:THR52
|
1.9
|
66.2
|
1.0
|
F1
|
I:BEF409
|
2.1
|
72.7
|
1.0
|
O1B
|
H:ADP408
|
2.1
|
74.2
|
1.0
|
OE2
|
H:GLU127
|
3.1
|
71.6
|
1.0
|
OD1
|
H:ASP126
|
3.1
|
75.7
|
1.0
|
CB
|
H:THR52
|
3.2
|
66.8
|
1.0
|
BE
|
I:BEF409
|
3.4
|
72.7
|
1.0
|
PB
|
H:ADP408
|
3.6
|
74.4
|
1.0
|
O2A
|
H:ADP408
|
3.7
|
74.7
|
1.0
|
NH2
|
I:ARG169
|
3.8
|
72.4
|
1.0
|
CG
|
H:ASP126
|
3.9
|
75.9
|
1.0
|
CG2
|
H:THR52
|
3.9
|
67.5
|
1.0
|
OD2
|
H:ASP126
|
3.9
|
75.6
|
1.0
|
O3B
|
H:ADP408
|
4.0
|
74.1
|
1.0
|
F2
|
I:BEF409
|
4.0
|
72.6
|
1.0
|
CD
|
H:GLU127
|
4.3
|
71.8
|
1.0
|
CA
|
H:THR52
|
4.3
|
67.5
|
1.0
|
N
|
H:THR52
|
4.3
|
67.2
|
1.0
|
OE2
|
I:GLU144
|
4.4
|
86.1
|
1.0
|
O3A
|
H:ADP408
|
4.5
|
74.6
|
1.0
|
F3
|
I:BEF409
|
4.5
|
72.9
|
1.0
|
OE1
|
I:GLU144
|
4.5
|
86.8
|
1.0
|
O2B
|
H:ADP408
|
4.7
|
75.0
|
1.0
|
NH2
|
H:ARG215
|
4.7
|
67.6
|
1.0
|
PA
|
H:ADP408
|
4.7
|
75.0
|
1.0
|
OE1
|
H:GLU127
|
4.9
|
71.8
|
1.0
|
CD
|
I:GLU144
|
4.9
|
86.0
|
1.0
|
|
Magnesium binding site 6 out
of 6 in 3glg
Go back to
Magnesium Binding Sites List in 3glg
Magnesium binding site 6 out
of 6 in the Crystal Structure of A Mutant (GAMMAT157A) E. Coli Clamp Loader Bound to Primer-Template Dna
Mono view
Stereo pair view
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 6 of Crystal Structure of A Mutant (GAMMAT157A) E. Coli Clamp Loader Bound to Primer-Template Dna within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
I:Mg417
b:67.6
occ:1.00
|
F1
|
I:BEF411
|
2.1
|
76.3
|
1.0
|
O1B
|
I:ADP410
|
2.1
|
67.6
|
1.0
|
CG2
|
I:THR52
|
2.4
|
73.6
|
1.0
|
OD2
|
I:ASP126
|
3.2
|
87.0
|
1.0
|
BE
|
I:BEF411
|
3.4
|
77.1
|
1.0
|
NH2
|
J:ARG158
|
3.4
|
73.7
|
1.0
|
OG1
|
I:THR52
|
3.4
|
75.5
|
1.0
|
OE2
|
I:GLU127
|
3.5
|
90.2
|
1.0
|
CB
|
I:THR52
|
3.5
|
74.5
|
1.0
|
PB
|
I:ADP410
|
3.5
|
68.0
|
1.0
|
O2A
|
I:ADP410
|
3.6
|
69.8
|
1.0
|
O3B
|
I:ADP410
|
3.9
|
68.0
|
1.0
|
F2
|
I:BEF411
|
3.9
|
78.1
|
1.0
|
OE1
|
J:GLU133
|
4.0
|
95.5
|
1.0
|
OD1
|
I:ASP126
|
4.0
|
85.5
|
1.0
|
CG
|
I:ASP126
|
4.0
|
86.2
|
1.0
|
NZ
|
J:LYS130
|
4.3
|
93.2
|
1.0
|
NH2
|
I:ARG215
|
4.4
|
74.7
|
1.0
|
N
|
I:THR52
|
4.5
|
74.7
|
1.0
|
O2B
|
I:ADP410
|
4.6
|
67.6
|
1.0
|
O3A
|
I:ADP410
|
4.6
|
69.4
|
1.0
|
OE2
|
J:GLU133
|
4.6
|
96.5
|
1.0
|
CA
|
I:THR52
|
4.6
|
74.9
|
1.0
|
CZ
|
J:ARG158
|
4.7
|
73.4
|
1.0
|
F3
|
I:BEF411
|
4.7
|
76.7
|
1.0
|
CD
|
J:GLU133
|
4.7
|
96.1
|
1.0
|
PA
|
I:ADP410
|
4.7
|
70.3
|
1.0
|
CD
|
I:GLU127
|
4.7
|
90.1
|
1.0
|
|
Reference:
K.R.Simonetta,
S.L.Kazmirski,
E.R.Goedken,
A.J.Cantor,
B.A.Kelch,
R.Mcnally,
S.N.Seyedin,
D.L.Makino,
M.O'donnell,
J.Kuriyan.
The Mechanism of Atp-Dependent Primer-Template Recognition By A Clamp Loader Complex. Cell(Cambridge,Mass.) V. 137 659 2009.
ISSN: ISSN 0092-8674
PubMed: 19450514
DOI: 10.1016/J.CELL.2009.03.044
Page generated: Wed Aug 14 14:49:59 2024
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