Magnesium in PDB 3gx2: Ttesam-I Riboswitch Variant A94GU34C Bound to Sinefungin

The structure of Ttesam-I Riboswitch Variant A94GU34C Bound to Sinefungin, PDB code: 3gx2 was solved by R.K.Montange, R.T.Batey, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.41 / 2.90
Space group	P 43 21 2
Cell size a, b, c (Å), α, β, γ (°)	62.614, 62.614, 158.395, 90.00, 90.00, 90.00
R / Rfree (%)	22.2 / 28.8

The binding sites of Magnesium atom in the Ttesam-I Riboswitch Variant A94GU34C Bound to Sinefungin (pdb code 3gx2). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 7 binding sites of Magnesium where determined in the Ttesam-I Riboswitch Variant A94GU34C Bound to Sinefungin, PDB code: 3gx2:
Jump to Magnesium binding site number: 1; 2; 3; 4; 5; 6; 7;

Magnesium binding site 1 out of 7 in the Ttesam-I Riboswitch Variant A94GU34C Bound to Sinefungin


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Ttesam-I Riboswitch Variant A94GU34C Bound to Sinefungin within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg201 b:74.8 occ:1.00 O6 A:G56 3.6 70.8 1.0 N7 A:G55 3.7 0.0 1.0 N7 A:G56 3.8 82.8 1.0 C2 A:A46 4.1 69.5 1.0 C5 A:G55 4.2 97.4 1.0 O6 A:G55 4.2 72.7 1.0 N1 A:A45 4.2 69.7 1.0 C8 A:G55 4.4 0.3 1.0 C6 A:G56 4.4 73.2 1.0 N1 A:A46 4.4 75.7 1.0 C6 A:G55 4.4 79.7 1.0 C5 A:G56 4.5 78.6 1.0 C8 A:G56 4.9 89.2 1.0 C2 A:A45 5.0 63.3 1.0 N6 A:A45 5.0 66.6 1.0

Magnesium binding site 2 out of 7 in the Ttesam-I Riboswitch Variant A94GU34C Bound to Sinefungin


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Ttesam-I Riboswitch Variant A94GU34C Bound to Sinefungin within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg202 b:0.1 occ:1.00 OP2 A:A53 3.0 0.5 1.0 N1 A:G50 3.2 86.6 1.0 C5' A:A52 3.4 0.8 1.0 N2 A:G50 3.4 76.7 1.0 O4 A:U54 3.5 0.6 1.0 P A:A53 3.7 0.2 1.0 OP1 A:A53 3.8 0.2 1.0 O3' A:A52 3.8 0.4 1.0 C2 A:G50 3.8 83.4 1.0 C3' A:A52 4.1 0.7 1.0 C6 A:G50 4.2 85.6 1.0 C4' A:A52 4.2 0.5 1.0 C4 A:U54 4.3 99.6 1.0 O6 A:G50 4.3 82.3 1.0 O5' A:A52 4.4 0.9 1.0 C5 A:U54 4.5 0.1 1.0

Magnesium binding site 3 out of 7 in the Ttesam-I Riboswitch Variant A94GU34C Bound to Sinefungin


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Ttesam-I Riboswitch Variant A94GU34C Bound to Sinefungin within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg203 b:58.0 occ:1.00 O2 A:U64 3.0 48.4 1.0 O4 A:U67 3.1 67.4 1.0 O2' A:U64 3.6 50.5 1.0 C4 A:U67 4.0 50.6 1.0 C2 A:U64 4.0 50.2 1.0 N6 A:A24 4.3 65.0 1.0 O6 A:G68 4.3 82.2 1.0 N4 A:C25 4.3 61.7 1.0 C5 A:U67 4.4 47.5 1.0 C2 A:A85 4.6 52.0 1.0 N3 A:U64 4.8 56.6 1.0 C2' A:U64 4.8 50.1 1.0 C1' A:U64 4.8 47.7 1.0 N7 A:A24 4.8 58.5 1.0 OP2 A:C66 4.9 56.3 1.0 N1 A:U64 4.9 48.3 1.0

Magnesium binding site 4 out of 7 in the Ttesam-I Riboswitch Variant A94GU34C Bound to Sinefungin


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 4 of Ttesam-I Riboswitch Variant A94GU34C Bound to Sinefungin within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg204 b:61.7 occ:1.00 N6 A:A83 4.0 72.4 1.0 O6 A:G82 4.1 67.8 1.0 N7 A:G82 4.2 80.5 1.0 N7 A:A83 4.3 69.4 1.0 C6 A:G82 4.7 75.0 1.0 C5 A:G82 4.7 82.6 1.0 N6 A:A84 4.9 74.3 1.0 C6 A:A83 5.0 70.1 1.0 C5 A:A83 5.0 68.7 1.0

Magnesium binding site 5 out of 7 in the Ttesam-I Riboswitch Variant A94GU34C Bound to Sinefungin


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 5 of Ttesam-I Riboswitch Variant A94GU34C Bound to Sinefungin within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg205 b:69.3 occ:1.00 OP2 A:A33 4.1 77.4 1.0 OP2 A:G32 4.6 74.9 1.0 OP1 A:A33 4.7 69.2 1.0 P A:A33 5.0 74.1 1.0

Magnesium binding site 6 out of 7 in the Ttesam-I Riboswitch Variant A94GU34C Bound to Sinefungin


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 6 of Ttesam-I Riboswitch Variant A94GU34C Bound to Sinefungin within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg206 b:71.2 occ:1.00 O6 A:G27 3.5 61.8 1.0 O6 A:G28 4.0 58.2 1.0 O6 A:G23 4.1 79.2 1.0 N7 A:G27 4.1 67.4 1.0 OP2 A:U26 4.1 99.5 1.0 N7 A:G28 4.2 53.1 1.0 C6 A:G27 4.4 51.8 1.0 C6 A:G23 4.5 69.2 1.0 C5 A:U26 4.5 76.8 1.0 OP2 A:C25 4.5 85.7 1.0 C5 A:G27 4.6 53.5 1.0 C6 A:G28 4.7 54.5 1.0 C5 A:G28 4.8 53.0 1.0 C5 A:G23 5.0 68.4 1.0 N1 A:G23 5.0 66.4 1.0

Magnesium binding site 7 out of 7 in the Ttesam-I Riboswitch Variant A94GU34C Bound to Sinefungin


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 7 of Ttesam-I Riboswitch Variant A94GU34C Bound to Sinefungin within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg207 b:78.5 occ:1.00 OP2 A:U64 2.8 60.4 1.0 OP2 A:A10 2.9 98.2 1.0 OP1 A:A62 3.2 67.1 1.0 P A:U64 4.0 45.6 1.0 P A:A10 4.1 96.5 1.0 N7 A:G11 4.1 61.0 1.0 O6 A:G11 4.2 70.9 1.0 P A:A62 4.2 60.7 1.0 OP2 A:A62 4.3 58.8 1.0 OP1 A:A10 4.4 86.7 1.0 O3' A:U63 4.4 84.6 1.0 OP1 A:U64 4.6 42.7 1.0 N6 A:A12 4.7 70.7 1.0 C5 A:G11 4.9 63.5 1.0 C6 A:G11 4.9 65.3 1.0 O3' A:A9 5.0 0.2 1.0

R.K.Montange, E.Mondragon, D.Van Tyne, A.D.Garst, P.Ceres, R.T.Batey. Discrimination Between Closely Related Cellular Metabolites By the Sam-I Riboswitch. J.Mol.Biol. V. 396 761 2010.
ISSN: ISSN 0022-2836
PubMed: 20006621
DOI: 10.1016/J.JMB.2009.12.007