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Magnesium in PDB 3h0g: Rna Polymerase II From Schizosaccharomyces Pombe

Enzymatic activity of Rna Polymerase II From Schizosaccharomyces Pombe

All present enzymatic activity of Rna Polymerase II From Schizosaccharomyces Pombe:
2.7.7.6;

Protein crystallography data

The structure of Rna Polymerase II From Schizosaccharomyces Pombe, PDB code: 3h0g was solved by H.Spahr, G.Calero, D.A.Bushnell, R.D.Kornberg, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 48.70 / 3.65
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 163.034, 202.684, 391.258, 90.00, 90.00, 90.00
R / Rfree (%) 29.7 / 32.1

Other elements in 3h0g:

The structure of Rna Polymerase II From Schizosaccharomyces Pombe also contains other interesting chemical elements:

Zinc (Zn) 16 atoms

Magnesium Binding Sites:

The binding sites of Magnesium atom in the Rna Polymerase II From Schizosaccharomyces Pombe (pdb code 3h0g). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Rna Polymerase II From Schizosaccharomyces Pombe, PDB code: 3h0g:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in 3h0g

Go back to Magnesium Binding Sites List in 3h0g
Magnesium binding site 1 out of 2 in the Rna Polymerase II From Schizosaccharomyces Pombe


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Rna Polymerase II From Schizosaccharomyces Pombe within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg2458

b:0.5
occ:1.00
OD2 A:ASP489 4.5 0.5 1.0
NH2 B:ARG1009 4.8 0.5 1.0

Magnesium binding site 2 out of 2 in 3h0g

Go back to Magnesium Binding Sites List in 3h0g
Magnesium binding site 2 out of 2 in the Rna Polymerase II From Schizosaccharomyces Pombe


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Rna Polymerase II From Schizosaccharomyces Pombe within 5.0Å range:
probe atom residue distance (Å) B Occ
M:Mg2458

b:0.4
occ:1.00
OD2 M:ASP489 4.5 0.6 1.0
NH2 N:ARG1009 4.9 0.4 1.0

Reference:

H.Spahr, G.Calero, D.A.Bushnell, R.D.Kornberg. Schizosacharomyces Pombe Rna Polymerase II at 3.6-A Resolution. Proc.Natl.Acad.Sci.Usa V. 106 9185 2009.
ISSN: ISSN 0027-8424
PubMed: 19458260
DOI: 10.1073/PNAS.0903361106
Page generated: Mon Dec 14 08:13:00 2020

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