Magnesium in PDB 3h8a: Crystal Structure of E. Coli Enolase Bound to Its Cognate Rnase E Recognition Domain

All present enzymatic activity of Crystal Structure of E. Coli Enolase Bound to Its Cognate Rnase E Recognition Domain:
4.2.1.11;

The structure of Crystal Structure of E. Coli Enolase Bound to Its Cognate Rnase E Recognition Domain, PDB code: 3h8a was solved by S.Nurmohamed, B.F.Luisi, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	24.89 / 1.90
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	103.883, 110.207, 160.267, 90.00, 90.00, 90.00
R / Rfree (%)	18 / 22.6

The binding sites of Magnesium atom in the Crystal Structure of E. Coli Enolase Bound to Its Cognate Rnase E Recognition Domain (pdb code 3h8a). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of E. Coli Enolase Bound to Its Cognate Rnase E Recognition Domain, PDB code: 3h8a:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of E. Coli Enolase Bound to Its Cognate Rnase E Recognition Domain


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of E. Coli Enolase Bound to Its Cognate Rnase E Recognition Domain within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1431 b:25.1 occ:1.00 OE2 A:GLU289 2.3 19.6 1.0 OD2 A:ASP316 2.4 20.4 1.0 O A:HOH878 2.4 42.9 1.0 OD2 A:ASP245 2.5 21.1 1.0 O A:HOH512 2.6 22.2 1.0 CG A:ASP245 3.3 18.2 1.0 CG A:ASP316 3.3 20.9 1.0 CD A:GLU289 3.4 18.4 1.0 OD1 A:ASP245 3.5 19.0 1.0 NZ A:LYS392 3.7 17.8 1.0 CB A:ASP316 3.8 18.9 1.0 O A:HOH530 3.8 30.2 1.0 O A:HOH608 4.0 36.9 1.0 OE1 A:GLU289 4.1 22.6 1.0 NE2 A:GLN166 4.2 20.0 1.0 CG A:GLU289 4.3 16.3 1.0 OD1 A:ASP316 4.3 22.3 1.0 OD2 A:ASP290 4.4 14.1 1.0 CB A:ASP245 4.5 15.6 1.0 O A:HOH581 4.5 27.8 1.0 CD2 A:LEU339 4.5 17.8 1.0 NZ A:LYS341 4.7 20.9 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of E. Coli Enolase Bound to Its Cognate Rnase E Recognition Domain


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of E. Coli Enolase Bound to Its Cognate Rnase E Recognition Domain within 5.0Å range: probe atom residue distance (Å) B Occ C:Mg1431 b:39.0 occ:1.00 OE2 C:GLU289 2.2 30.1 1.0 O C:HOH879 2.2 31.9 1.0 OD2 C:ASP245 2.2 37.6 1.0 OD2 C:ASP316 2.5 36.1 1.0 CG C:ASP245 3.2 36.0 1.0 CD C:GLU289 3.3 29.8 1.0 OD1 C:ASP245 3.5 39.3 1.0 CG C:ASP316 3.5 35.9 1.0 NZ C:LYS392 4.0 21.0 1.0 CB C:ASP316 4.0 35.6 1.0 OD2 C:ASP290 4.0 33.9 1.0 OE1 C:GLU289 4.1 31.4 1.0 CG C:GLU289 4.2 29.5 1.0 O C:HOH926 4.3 41.9 1.0 NE2 C:GLN166 4.3 34.9 1.0 O C:HOH1187 4.4 48.8 1.0 NZ C:LYS341 4.4 23.7 1.0 CB C:ASP245 4.4 34.9 1.0 CD2 C:LEU339 4.5 23.8 1.0 OD1 C:ASP316 4.6 36.8 1.0

S.Nurmohamed, A.R.Mckay, C.V.Robinson, B.F.Luisi. Molecular Recognition Between Escherichia Coli Enolase and Ribonuclease E. Acta Crystallogr.,Sect.D V. 66 1036 2010.
ISSN: ISSN 0907-4449
PubMed: 20823555
DOI: 10.1107/S0907444910030015