Magnesium in PDB 3hb1: Crystal Structure of Ed-EYA2 Complexed with ALF3

All present enzymatic activity of Crystal Structure of Ed-EYA2 Complexed with ALF3:
3.1.3.48;

The structure of Crystal Structure of Ed-EYA2 Complexed with ALF3, PDB code: 3hb1 was solved by S.K.Jung, D.G.Jeong, S.E.Ryu, S.J.Kim, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	40.00 / 2.51
Space group	I 4
Cell size a, b, c (Å), α, β, γ (°)	183.941, 183.941, 119.976, 90.00, 90.00, 90.00
R / Rfree (%)	20 / 24

The structure of Crystal Structure of Ed-EYA2 Complexed with ALF3 also contains other interesting chemical elements:

Fluorine	(F)	12 atoms
Aluminium	(Al)	4 atoms

The binding sites of Magnesium atom in the Crystal Structure of Ed-EYA2 Complexed with ALF3 (pdb code 3hb1). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 4 binding sites of Magnesium where determined in the Crystal Structure of Ed-EYA2 Complexed with ALF3, PDB code: 3hb1:
Jump to Magnesium binding site number: 1; 2; 3; 4;

Magnesium binding site 1 out of 4 in the Crystal Structure of Ed-EYA2 Complexed with ALF3


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of Ed-EYA2 Complexed with ALF3 within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg701 b:26.8 occ:1.00 O A:HOH143 1.8 36.7 1.0 OD2 A:ASP274 2.0 34.0 1.0 OD1 A:ASP502 2.1 30.6 1.0 F1 A:AF3801 2.2 45.1 1.0 O A:ASP276 2.2 31.8 1.0 CG A:ASP274 3.0 33.8 1.0 CG A:ASP502 3.0 28.5 1.0 OD2 A:ASP502 3.3 25.6 1.0 C A:ASP276 3.4 29.4 1.0 OD1 A:ASP274 3.4 37.4 1.0 OE2 A:GLU506 3.5 44.1 1.0 AL A:AF3801 3.6 41.8 1.0 OG1 A:THR278 3.8 23.2 1.0 CA A:ASP276 4.1 30.6 1.0 O A:HOH36 4.2 22.1 1.0 CB A:ASP276 4.2 30.5 1.0 N A:ASP276 4.2 30.1 1.0 CB A:ASP274 4.3 29.7 1.0 CB A:GLU277 4.3 24.0 1.0 CB A:ASP502 4.4 28.7 1.0 N A:GLU277 4.4 26.9 1.0 N A:GLY503 4.4 30.9 1.0 CD A:GLU506 4.5 39.2 1.0 O A:HOH125 4.5 47.4 1.0 F3 A:AF3801 4.6 42.3 1.0 N A:THR278 4.6 22.5 1.0 C A:GLU277 4.6 23.8 1.0 CA A:GLU277 4.6 24.7 1.0 N A:ASP502 4.7 27.5 1.0 OE1 A:GLU506 4.7 40.6 1.0 O A:HOH75 4.8 35.7 1.0 CB A:THR278 4.8 22.3 1.0 F2 A:AF3801 4.8 44.5 1.0 C A:LEU275 4.8 28.4 1.0 CA A:ASP502 4.9 28.7 1.0 N A:LEU275 5.0 26.5 1.0 C A:ASP502 5.0 30.0 1.0

Magnesium binding site 2 out of 4 in the Crystal Structure of Ed-EYA2 Complexed with ALF3


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of Ed-EYA2 Complexed with ALF3 within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg702 b:35.2 occ:1.00 F1 B:AF3802 2.0 46.0 1.0 OD1 B:ASP502 2.0 35.7 1.0 O B:HOH137 2.0 37.4 1.0 O B:ASP276 2.1 35.2 1.0 OD2 B:ASP274 2.3 39.4 1.0 CG B:ASP502 2.9 34.2 1.0 OD2 B:ASP502 3.1 35.2 1.0 CG B:ASP274 3.3 40.9 1.0 C B:ASP276 3.3 35.5 1.0 AL B:AF3802 3.6 46.5 1.0 OD1 B:ASP274 3.6 43.9 1.0 OG1 B:THR278 4.0 29.4 1.0 CB B:ASP276 4.1 40.8 1.0 CA B:ASP276 4.1 36.3 1.0 O B:HOH135 4.1 34.9 1.0 N B:GLY503 4.3 41.7 1.0 CB B:ASP502 4.3 35.2 1.0 N B:GLU277 4.3 34.2 1.0 CB B:GLU277 4.3 32.8 1.0 N B:ASP276 4.4 35.3 1.0 OE2 B:GLU506 4.4 50.3 1.0 F2 B:AF3802 4.6 50.2 1.0 CB B:ASP274 4.6 38.1 1.0 CA B:GLU277 4.6 32.7 1.0 OE1 B:GLU506 4.6 47.8 1.0 N B:ASP502 4.7 35.3 1.0 C B:GLU277 4.7 32.0 1.0 F3 B:AF3802 4.7 44.9 1.0 C B:ASP502 4.8 39.5 1.0 CA B:GLY503 4.8 42.6 1.0 CD B:GLU506 4.9 48.7 1.0 CA B:ASP502 4.9 37.0 1.0 N B:THR278 4.9 29.5 1.0

Magnesium binding site 3 out of 4 in the Crystal Structure of Ed-EYA2 Complexed with ALF3


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Crystal Structure of Ed-EYA2 Complexed with ALF3 within 5.0Å range: probe atom residue distance (Å) B Occ C:Mg703 b:24.1 occ:1.00 OD1 C:ASP502 2.1 38.5 1.0 O C:HOH142 2.1 42.5 1.0 F1 C:AF3803 2.1 49.9 1.0 OD2 C:ASP274 2.1 39.2 1.0 O C:ASP276 2.2 37.7 1.0 CG C:ASP502 2.9 36.1 1.0 CG C:ASP274 3.0 39.0 1.0 OD2 C:ASP502 3.1 36.0 1.0 AL C:AF3803 3.3 48.0 1.0 OD1 C:ASP274 3.3 43.5 1.0 C C:ASP276 3.4 37.2 1.0 F3 C:AF3803 3.7 49.2 1.0 OE2 C:GLU506 3.7 53.8 1.0 O C:HOH131 4.1 34.2 1.0 CA C:ASP276 4.1 38.1 1.0 OG1 C:THR278 4.1 32.1 1.0 CB C:ASP276 4.2 40.7 1.0 N C:ASP276 4.2 37.2 1.0 N C:GLY503 4.2 37.2 1.0 CB C:ASP274 4.4 35.7 1.0 CB C:ASP502 4.4 34.5 1.0 CD C:GLU506 4.4 50.1 1.0 N C:GLU277 4.5 35.5 1.0 CB C:GLU277 4.5 32.9 1.0 OE1 C:GLU506 4.5 52.1 1.0 N C:ASP502 4.7 33.1 1.0 F2 C:AF3803 4.8 44.7 1.0 C C:GLU277 4.8 33.5 1.0 CA C:GLY503 4.8 38.6 1.0 CA C:GLU277 4.8 33.4 1.0 N C:THR278 4.8 32.8 1.0 C C:ASP502 4.9 35.9 1.0 CA C:ASP502 4.9 34.2 1.0 C C:LEU275 5.0 36.7 1.0

Magnesium binding site 4 out of 4 in the Crystal Structure of Ed-EYA2 Complexed with ALF3


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 4 of Crystal Structure of Ed-EYA2 Complexed with ALF3 within 5.0Å range: probe atom residue distance (Å) B Occ D:Mg704 b:32.5 occ:1.00 F1 D:AF3804 1.9 48.6 1.0 OD2 D:ASP274 1.9 42.2 1.0 O D:HOH139 2.1 25.4 1.0 O D:ASP276 2.2 34.9 1.0 OD1 D:ASP502 2.3 37.7 1.0 CG D:ASP274 2.8 41.3 1.0 OD1 D:ASP274 3.1 44.8 1.0 CG D:ASP502 3.2 35.7 1.0 AL D:AF3804 3.3 47.6 1.0 C D:ASP276 3.3 36.1 1.0 OD2 D:ASP502 3.4 36.8 1.0 OE2 D:GLU506 3.4 47.5 1.0 CA D:ASP276 3.9 36.4 1.0 OG1 D:THR278 4.0 28.3 1.0 CB D:ASP276 4.0 39.4 1.0 N D:ASP276 4.1 35.1 1.0 O D:HOH140 4.1 44.3 1.0 CB D:ASP274 4.1 34.7 1.0 F3 D:AF3804 4.2 50.2 1.0 CD D:GLU506 4.4 45.1 1.0 F2 D:AF3804 4.4 44.0 1.0 N D:GLU277 4.4 34.7 1.0 N D:GLY503 4.5 36.4 1.0 CB D:ASP502 4.6 33.4 1.0 CB D:GLU277 4.6 33.6 1.0 OE1 D:GLU506 4.6 46.4 1.0 C D:GLU277 4.8 32.1 1.0 C D:LEU275 4.8 33.0 1.0 CA D:GLU277 4.8 33.3 1.0 N D:ASP502 4.8 30.9 1.0 N D:THR278 4.8 30.0 1.0 N D:LEU275 4.9 32.7 1.0 CB D:THR278 5.0 26.2 1.0

S.K.Jung, D.G.Jeong, S.J.Chung, J.H.Kim, B.C.Park, N.K.Tonks, S.E.Ryu, S.J.Kim. Crystal Structure of Ed-EYA2: Insight Into Dual Roles As A Protein Tyrosine Phosphatase and A Transcription Factor Faseb J. V. 24 560 2010.
ISSN: ISSN 0892-6638
PubMed: 19858093
DOI: 10.1096/FJ.09-143891