Magnesium in PDB 3hox: Complete Rna Polymerase II Elongation Complex V

All present enzymatic activity of Complete Rna Polymerase II Elongation Complex V:
2.7.7.6;

The structure of Complete Rna Polymerase II Elongation Complex V, PDB code: 3hox was solved by J.F.Sydow, F.Brueckner, A.C.M.Cheung, G.E.Damsma, S.Dengl, E.Lehmann, D.Vassylyev, P.Cramer, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.00 / 3.65
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	221.617, 393.716, 282.576, 90.00, 90.00, 90.00
R / Rfree (%)	21.2 / 25

The structure of Complete Rna Polymerase II Elongation Complex V also contains other interesting chemical elements:

Bromine	(Br)	1 atom
Zinc	(Zn)	8 atoms

The binding sites of Magnesium atom in the Complete Rna Polymerase II Elongation Complex V (pdb code 3hox). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the Complete Rna Polymerase II Elongation Complex V, PDB code: 3hox:

Magnesium binding site 1 out of 1 in the Complete Rna Polymerase II Elongation Complex V


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Complete Rna Polymerase II Elongation Complex V within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg2458 b:0.5 occ:1.00 OD1 A:ASP481 2.3 99.1 1.0 OD2 A:ASP485 2.3 87.5 1.0 OD2 A:ASP483 2.3 1.0 1.0 OD1 A:ASP485 2.4 88.2 1.0 OP1 P:U11 2.4 0.8 1.0 O3' P:U10 2.5 0.0 1.0 CG A:ASP485 2.7 85.5 1.0 P P:U11 2.9 0.5 1.0 CG A:ASP483 3.3 0.4 1.0 CG A:ASP481 3.4 98.7 1.0 O5' P:U11 3.4 0.6 1.0 C3' P:U10 3.9 0.9 1.0 OD2 A:ASP481 3.9 98.8 1.0 OD1 A:ASP483 4.1 0.8 1.0 CB A:ASP485 4.2 80.3 1.0 O2' P:U10 4.2 0.5 1.0 CB A:ASP483 4.2 0.3 1.0 OP2 P:U11 4.3 0.5 1.0 C2' P:U10 4.6 0.1 1.0 N A:ASP483 4.6 97.6 1.0 CB A:ASP481 4.6 97.6 1.0 C4' P:U10 4.6 0.3 1.0 O A:ASP481 4.7 98.6 1.0 NH2 A:ARG446 4.9 80.2 1.0 N A:ASP485 4.9 79.9 1.0 CA A:ASP485 4.9 76.6 1.0 CA A:ASP483 4.9 97.3 1.0 C A:ASP481 5.0 98.2 1.0

J.F.Sydow, F.Brueckner, A.C.M.Cheung, G.E.Damsma, S.Dengl, E.Lehmann, D.Vassylyev, P.Cramer. Structural Basis of Transcription: Mismatch-Specific Fidelity Mechanisms and Paused Rna Polymerase II with Frayed Rna. Mol.Cell V. 34 710 2009.
ISSN: ISSN 1097-2765
PubMed: 19560423
DOI: 10.1016/J.MOLCEL.2009.06.002