Magnesium in PDB 3htx: Crystal Structure of Small Rna Methyltransferase HEN1

The structure of Crystal Structure of Small Rna Methyltransferase HEN1, PDB code: 3htx was solved by Y.Huang, L.-J.Ji, Q.-C.Huang, D.G.Vassylyev, X.-M.Chen, J.-B.Ma, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 3.10
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	96.995, 124.347, 101.376, 90.00, 93.47, 90.00
R / Rfree (%)	26 / 28.8

The binding sites of Magnesium atom in the Crystal Structure of Small Rna Methyltransferase HEN1 (pdb code 3htx). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of Small Rna Methyltransferase HEN1, PDB code: 3htx:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of Small Rna Methyltransferase HEN1


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of Small Rna Methyltransferase HEN1 within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg950 b:60.1 occ:1.00 OE2 A:GLU799 2.2 0.0 1.0 OE1 A:GLU796 2.2 0.2 1.0 O2' B:G22 2.2 86.3 1.0 NE2 A:HIS860 2.2 95.7 1.0 O3' B:G22 2.2 89.2 1.0 NE2 A:HIS800 2.2 80.9 1.0 CD A:GLU799 2.8 0.4 1.0 C3' B:G22 2.8 90.3 1.0 OE1 A:GLU799 2.8 0.3 1.0 C2' B:G22 2.9 89.7 1.0 CE1 A:HIS860 2.9 96.1 1.0 CD2 A:HIS800 3.0 81.4 1.0 CE1 A:HIS800 3.0 80.7 1.0 CD2 A:HIS860 3.2 95.8 1.0 CD A:GLU796 3.4 98.7 1.0 C4' B:G22 3.6 91.5 1.0 O A:GLU796 3.6 87.6 1.0 ND1 A:HIS800 4.0 80.2 1.0 OE2 A:GLU796 4.0 0.5 1.0 CG A:HIS800 4.0 80.4 1.0 ND1 A:HIS860 4.0 96.9 1.0 C1' B:G22 4.2 91.8 1.0 CG A:HIS860 4.2 95.4 1.0 CG A:GLU799 4.3 95.1 1.0 O4' B:G22 4.4 91.4 1.0 CG A:GLU796 4.5 95.4 1.0 C5' B:G22 4.7 93.2 1.0 CB A:GLU796 4.7 93.3 1.0 C A:GLU796 4.8 89.0 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of Small Rna Methyltransferase HEN1


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of Small Rna Methyltransferase HEN1 within 5.0Å range: probe atom residue distance (Å) B Occ D:Mg950 b:36.6 occ:1.00 OE2 D:GLU799 2.2 88.8 1.0 O2' E:G22 2.2 0.7 1.0 OE1 D:GLU796 2.2 90.7 1.0 NE2 D:HIS800 2.2 92.7 1.0 O3' E:G22 2.2 0.9 1.0 NE2 D:HIS860 2.2 92.0 1.0 CE1 D:HIS860 2.3 92.5 1.0 C2' E:G22 3.0 0.7 1.0 CD D:GLU799 3.1 87.2 1.0 CD2 D:HIS800 3.1 92.7 1.0 C3' E:G22 3.1 0.1 1.0 CE1 D:HIS800 3.2 92.7 1.0 CD2 D:HIS860 3.4 93.3 1.0 ND1 D:HIS860 3.4 93.2 1.0 CD D:GLU796 3.4 88.8 1.0 CG D:GLU799 3.5 86.6 1.0 O D:GLU796 3.7 81.3 1.0 C4' E:G22 3.8 0.0 1.0 CG D:HIS860 3.9 93.6 1.0 CB D:GLU796 4.0 81.6 1.0 OE1 D:GLU799 4.1 85.8 1.0 CG D:GLU796 4.2 86.1 1.0 C D:GLU796 4.2 82.0 1.0 CG D:HIS800 4.2 92.6 1.0 ND1 D:HIS800 4.2 92.6 1.0 CA D:GLU796 4.3 81.8 1.0 C1' E:G22 4.3 0.1 1.0 OE2 D:GLU796 4.4 89.1 1.0 CG D:PRO827 4.5 90.7 1.0 O4' E:G22 4.7 0.6 1.0 CD D:PRO827 4.8 90.4 1.0

Y.Huang, L.Ji, Q.Huang, D.G.Vassylyev, X.Chen, J.B.Ma. Structural Insights Into Mechanisms of the Small Rna Methyltransferase HEN1. Nature V. 461 823 2009.
ISSN: ISSN 0028-0836
PubMed: 19812675
DOI: 10.1038/NATURE08433