Magnesium in PDB 3i4n: 8-Oxoguanine Containing Rna Polymerase II Elongation Complex E

All present enzymatic activity of 8-Oxoguanine Containing Rna Polymerase II Elongation Complex E:
2.7.7.6;

The structure of 8-Oxoguanine Containing Rna Polymerase II Elongation Complex E, PDB code: 3i4n was solved by G.E.Damsma, P.Cramer, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.00 / 3.90
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	221.173, 394.148, 282.339, 90.00, 90.00, 90.00
R / Rfree (%)	22.8 / 26.6

The structure of 8-Oxoguanine Containing Rna Polymerase II Elongation Complex E also contains other interesting chemical elements:

Bromine	(Br)	1 atom
Zinc	(Zn)	8 atoms

The binding sites of Magnesium atom in the 8-Oxoguanine Containing Rna Polymerase II Elongation Complex E (pdb code 3i4n). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the 8-Oxoguanine Containing Rna Polymerase II Elongation Complex E, PDB code: 3i4n:

Magnesium binding site 1 out of 1 in the 8-Oxoguanine Containing Rna Polymerase II Elongation Complex E


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of 8-Oxoguanine Containing Rna Polymerase II Elongation Complex E within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg2458 b:0.0 occ:1.00 OP2 P:U11 2.2 0.0 1.0 OD2 A:ASP483 2.3 0.7 1.0 OD2 A:ASP485 2.4 0.1 1.0 OD1 A:ASP481 2.4 0.0 1.0 O3' P:A10 2.4 0.7 1.0 CG A:ASP483 2.6 0.9 1.0 OD1 A:ASP485 2.7 0.4 1.0 OD1 A:ASP483 2.7 0.1 1.0 P P:U11 2.8 0.0 1.0 CG A:ASP485 2.8 0.4 1.0 CG A:ASP481 3.3 0.5 1.0 C5' P:U11 3.6 0.0 1.0 O5' P:U11 3.6 0.0 1.0 OD2 A:ASP481 3.7 0.6 1.0 C3' P:A10 3.8 0.7 1.0 O A:ASP483 3.9 0.1 1.0 CB A:ASP483 3.9 0.5 1.0 C A:ASP483 4.1 0.9 1.0 N A:ASP483 4.1 0.0 1.0 OP1 P:U11 4.1 0.0 1.0 CA A:ASP483 4.2 0.5 1.0 C4' P:U11 4.2 0.0 1.0 O4' P:U11 4.2 0.0 1.0 CB A:ASP485 4.2 0.6 1.0 C4' P:A10 4.3 0.6 1.0 CB A:ASP481 4.4 0.0 1.0 O2' P:A10 4.4 0.0 1.0 N A:ASP485 4.6 0.4 1.0 C2' P:A10 4.7 0.0 1.0 C5' P:A10 4.8 0.8 1.0 N A:GLY484 4.8 0.5 1.0 CA A:ASP485 4.8 0.8 1.0 O A:ASP481 4.9 0.8 1.0 C A:ASP481 4.9 0.2 1.0 NH2 A:ARG446 4.9 0.1 1.0

G.E.Damsma, P.Cramer. Molecular Basis of Transcriptional Mutagenesis at 8-Oxoguanine J.Biol.Chem. V. 284 31658 2009.
ISSN: ISSN 0021-9258
PubMed: 19758983
DOI: 10.1074/JBC.M109.022764