Magnesium in PDB 3ka6: Frog M-Ferritin, Eed Mutant, with Cobalt

All present enzymatic activity of Frog M-Ferritin, Eed Mutant, with Cobalt:
1.16.3.1;

The structure of Frog M-Ferritin, Eed Mutant, with Cobalt, PDB code: 3ka6 was solved by T.Tosha, H.L.Ng, E.Theil, T.Alber, O.Bhattasali, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.00 / 1.40
Space group	F 4 3 2
Cell size a, b, c (Å), α, β, γ (°)	183.708, 183.708, 183.708, 90.00, 90.00, 90.00
R / Rfree (%)	13.9 / 16.6

The structure of Frog M-Ferritin, Eed Mutant, with Cobalt also contains other interesting chemical elements:

Cobalt	(Co)	10 atoms
Chlorine	(Cl)	4 atoms

The binding sites of Magnesium atom in the Frog M-Ferritin, Eed Mutant, with Cobalt (pdb code 3ka6). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 5 binding sites of Magnesium where determined in the Frog M-Ferritin, Eed Mutant, with Cobalt, PDB code: 3ka6:
Jump to Magnesium binding site number: 1; 2; 3; 4; 5;

Magnesium binding site 1 out of 5 in the Frog M-Ferritin, Eed Mutant, with Cobalt


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Frog M-Ferritin, Eed Mutant, with Cobalt within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg186 b:13.2 occ:0.50 O A:HOH406 2.1 12.4 0.5 O A:HOH379 2.1 16.1 1.0 O A:HOH364 4.2 15.2 1.0 NZ A:LYS82 4.5 23.9 1.0 O A:HOH405 4.5 24.1 1.0 CE A:LYS83 4.6 15.3 1.0 O A:HOH463 4.7 29.8 1.0 CD A:LYS82 4.8 16.2 1.0

Magnesium binding site 2 out of 5 in the Frog M-Ferritin, Eed Mutant, with Cobalt


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Frog M-Ferritin, Eed Mutant, with Cobalt within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg187 b:22.9 occ:1.00 O A:HOH440 2.0 22.0 1.0 O A:HOH444 2.0 26.3 1.0 O A:HOH235 2.1 30.5 1.0 OG A:SER10 2.1 19.6 1.0 O A:HOH376 2.1 17.2 1.0 O A:HOH493 2.1 29.3 1.0 CB A:SER10 3.2 14.1 1.0 CA A:SER10 3.9 12.4 1.0 O A:SER10 4.1 11.9 1.0 OE1 A:GLU13 4.1 15.1 1.0 O A:HOH385 4.2 22.3 1.0 O A:HOH273 4.3 41.7 1.0 C A:SER10 4.3 10.6 1.0 O A:HOH263 4.3 38.2 1.0 O A:HOH381 4.5 19.5 1.0 O A:HOH297 4.5 42.6 1.0 CD A:GLU13 4.7 11.6 1.0 O A:HOH272 5.0 34.4 1.0

Magnesium binding site 3 out of 5 in the Frog M-Ferritin, Eed Mutant, with Cobalt


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Frog M-Ferritin, Eed Mutant, with Cobalt within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg188 b:28.9 occ:1.00 O A:HOH304 1.9 37.9 1.0 O A:HOH333 2.1 40.5 1.0 O A:HOH315 2.2 36.5 1.0 O A:HOH396 2.2 21.1 1.0 OD2 A:ASP146 4.0 14.9 1.0 O A:HOH281 4.2 47.0 1.0 O A:HOH472 4.2 25.8 1.0 O A:HOH398 4.3 18.2 1.0 O A:HOH470 4.3 31.8 1.0 O A:HOH335 4.4 36.2 1.0 O A:HOH336 4.4 74.6 1.0 O A:HOH418 4.5 22.6 1.0

Magnesium binding site 4 out of 5 in the Frog M-Ferritin, Eed Mutant, with Cobalt


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 4 of Frog M-Ferritin, Eed Mutant, with Cobalt within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg191 b:18.4 occ:1.00 O A:HOH299 2.1 21.2 1.0 O A:HOH253 2.1 21.2 1.0 O A:HOH334 2.1 25.5 1.0 O A:HOH211 4.1 36.4 1.0 O A:SER131 4.2 15.6 1.0 O A:HOH511 4.3 35.1 1.0 O A:GLU130 4.3 15.6 1.0 O A:HOH219 4.5 38.2 1.0 CA A:SER131 4.5 13.9 1.0 O A:HOH262 4.5 34.3 1.0 C A:SER131 4.7 13.8 1.0 CB A:GLU135 4.7 14.0 1.0 CL A:CL190 4.9 22.2 1.0 CG A:GLU135 4.9 14.2 1.0

Magnesium binding site 5 out of 5 in the Frog M-Ferritin, Eed Mutant, with Cobalt


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 5 of Frog M-Ferritin, Eed Mutant, with Cobalt within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg192 b:35.5 occ:1.00 O A:HOH340 2.0 34.1 1.0 O A:HOH414 2.2 21.0 1.0 O A:HOH341 2.3 48.2 1.0 O A:HOH238 3.8 34.6 1.0 O A:HOH436 3.9 19.4 1.0 O A:HOH252 4.5 21.8 1.0 CO A:CO181 4.9 22.6 0.5

T.Tosha, H.L.Ng, O.Bhattasali, T.Alber, E.C.Theil. Moving Metal Ions Through Ferritin-Protein Nanocages From Three-Fold Pores to Catalytic Sites. J.Am.Chem.Soc. V. 132 14562 2010.
ISSN: ISSN 0002-7863
PubMed: 20866049
DOI: 10.1021/JA105583D