Magnesium in PDB 3mjh: Crystal Structure of Human RAB5A in Complex with the C2H2 Zinc Finger of EEA1

All present enzymatic activity of Crystal Structure of Human RAB5A in Complex with the C2H2 Zinc Finger of EEA1:
3.6.5.2;

The structure of Crystal Structure of Human RAB5A in Complex with the C2H2 Zinc Finger of EEA1, PDB code: 3mjh was solved by A.K.Mishra, S.Eathiraj, D.G.Lambright, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 2.03
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	46.412, 80.398, 103.495, 90.00, 90.00, 90.00
R / Rfree (%)	19.2 / 26

The structure of Crystal Structure of Human RAB5A in Complex with the C2H2 Zinc Finger of EEA1 also contains other interesting chemical elements:

Zinc

(Zn)

2 atoms

The binding sites of Magnesium atom in the Crystal Structure of Human RAB5A in Complex with the C2H2 Zinc Finger of EEA1 (pdb code 3mjh). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of Human RAB5A in Complex with the C2H2 Zinc Finger of EEA1, PDB code: 3mjh:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of Human RAB5A in Complex with the C2H2 Zinc Finger of EEA1


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of Human RAB5A in Complex with the C2H2 Zinc Finger of EEA1 within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg201 b:30.2 occ:1.00 O A:HOH302 2.0 24.7 1.0 OG1 A:THR52 2.0 31.5 1.0 O2G A:GTP200 2.0 28.8 1.0 O2B A:GTP200 2.1 26.9 1.0 OG A:SER34 2.2 24.2 1.0 O A:HOH306 2.3 30.8 1.0 CB A:THR52 3.1 32.0 1.0 PG A:GTP200 3.2 31.3 1.0 CB A:SER34 3.2 23.7 1.0 PB A:GTP200 3.3 28.0 1.0 O3B A:GTP200 3.5 28.4 1.0 N A:THR52 3.7 32.5 1.0 O3G A:GTP200 3.9 29.6 1.0 N A:SER34 3.9 24.3 1.0 CA A:THR52 4.0 32.1 1.0 OD2 A:ASP75 4.1 26.4 1.0 CA A:SER34 4.1 23.9 1.0 OD1 A:ASP75 4.2 26.1 1.0 O2A A:GTP200 4.3 26.8 1.0 CG2 A:THR52 4.3 31.2 1.0 O1B A:GTP200 4.3 27.7 1.0 O3A A:GTP200 4.4 28.4 1.0 O1G A:GTP200 4.4 29.9 1.0 O B:HOH71 4.4 35.1 1.0 CG A:ASP75 4.6 26.1 1.0 O A:THR76 4.6 29.9 1.0 O A:GLU50 4.6 31.7 1.0 PA A:GTP200 4.6 26.6 1.0 C A:SER51 4.7 32.6 1.0 O1A A:GTP200 4.7 27.4 1.0 CB A:LYS33 4.9 25.3 1.0 CA A:SER51 5.0 33.0 1.0 CE A:LYS33 5.0 26.3 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of Human RAB5A in Complex with the C2H2 Zinc Finger of EEA1


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of Human RAB5A in Complex with the C2H2 Zinc Finger of EEA1 within 5.0Å range: probe atom residue distance (Å) B Occ C:Mg201 b:27.0 occ:1.00 O2B C:GTP200 2.0 29.2 1.0 O2G C:GTP200 2.1 31.1 1.0 OG C:SER34 2.1 27.1 1.0 O C:HOH274 2.1 25.9 1.0 O C:HOH272 2.2 26.2 1.0 OG1 C:THR52 2.3 31.1 1.0 CB C:SER34 3.2 26.9 1.0 PG C:GTP200 3.2 34.1 1.0 PB C:GTP200 3.2 30.2 1.0 CB C:THR52 3.2 32.1 1.0 O3B C:GTP200 3.4 31.6 1.0 N C:THR52 3.7 32.7 1.0 N C:SER34 3.8 27.0 1.0 O3G C:GTP200 3.9 32.8 1.0 CA C:SER34 4.1 26.7 1.0 O2A C:GTP200 4.1 30.2 1.0 CA C:THR52 4.1 32.3 1.0 OD2 C:ASP75 4.2 26.8 1.0 OD1 C:ASP75 4.2 27.6 1.0 O1B C:GTP200 4.2 29.5 1.0 O C:HOH220 4.2 28.7 1.0 O3A C:GTP200 4.3 31.6 1.0 O1G C:GTP200 4.3 32.2 1.0 CG2 C:THR52 4.4 31.5 1.0 PA C:GTP200 4.5 29.1 1.0 CG C:ASP75 4.6 28.7 1.0 O C:GLU50 4.6 33.7 1.0 O1A C:GTP200 4.6 30.0 1.0 O C:THR76 4.6 31.2 1.0 CB C:LYS33 4.7 27.8 1.0 C C:SER51 4.8 32.7 1.0 C C:LYS33 4.9 27.6 1.0

A.Mishra, S.Eathiraj, S.Corvera, D.G.Lambright. Structural Basis For Rab Gtpase Recognition and Endosome Tethering By the C2H2 Zinc Finger of Early Endosomal Autoantigen 1 (EEA1). Proc.Natl.Acad.Sci.Usa V. 107 10866 2010.
ISSN: ISSN 0027-8424
PubMed: 20534488
DOI: 10.1073/PNAS.1000843107