Atomistry » Magnesium » PDB 3mv1-3n8b » 3n23
Atomistry »
  Magnesium »
    PDB 3mv1-3n8b »
      3n23 »

Magnesium in PDB 3n23: Crystal Structure of the High Affinity Complex Between Ouabain and the E2P Form of the Sodium-Potassium Pump

Enzymatic activity of Crystal Structure of the High Affinity Complex Between Ouabain and the E2P Form of the Sodium-Potassium Pump

All present enzymatic activity of Crystal Structure of the High Affinity Complex Between Ouabain and the E2P Form of the Sodium-Potassium Pump:
3.6.3.9;

Protein crystallography data

The structure of Crystal Structure of the High Affinity Complex Between Ouabain and the E2P Form of the Sodium-Potassium Pump, PDB code: 3n23 was solved by L.Yatime, M.Laursen, J.P.Morth, M.Esmann, P.Nissen, N.U.Fedosova, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 39.55 / 4.60
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 117.160, 118.930, 494.100, 90.00, 90.00, 90.00
R / Rfree (%) 27.2 / 30.2

Magnesium Binding Sites:

The binding sites of Magnesium atom in the Crystal Structure of the High Affinity Complex Between Ouabain and the E2P Form of the Sodium-Potassium Pump (pdb code 3n23). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of the High Affinity Complex Between Ouabain and the E2P Form of the Sodium-Potassium Pump, PDB code: 3n23:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in 3n23

Go back to Magnesium Binding Sites List in 3n23
Magnesium binding site 1 out of 2 in the Crystal Structure of the High Affinity Complex Between Ouabain and the E2P Form of the Sodium-Potassium Pump


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of the High Affinity Complex Between Ouabain and the E2P Form of the Sodium-Potassium Pump within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg1017

b:0.7
occ:1.00
OP1 A:PHD369 2.5 0.4 1.0
OD2 A:ASP710 2.6 0.6 1.0
O A:THR371 2.7 0.2 1.0
OD1 A:PHD369 2.8 0.4 1.0
OD2 A:PHD369 2.9 0.4 1.0
CG A:PHD369 3.0 0.4 1.0
P A:PHD369 3.1 0.4 1.0
OP2 A:PHD369 3.6 0.4 1.0
N A:GLY711 3.6 0.0 1.0
CG A:ASP710 3.6 0.6 1.0
OD2 A:ASP714 3.7 0.6 1.0
C A:THR371 3.8 0.2 1.0
CA A:GLY711 3.8 0.0 1.0
CA A:GLY213 3.9 0.8 1.0
OD1 A:ASP710 4.0 0.6 1.0
CB A:THR371 4.1 0.3 1.0
O A:SER209 4.1 0.4 1.0
OD1 A:ASN713 4.2 0.8 1.0
CA A:THR371 4.3 0.2 1.0
N A:THR371 4.4 0.2 1.0
CB A:PHD369 4.5 0.4 1.0
OP3 A:PHD369 4.5 0.4 1.0
OD1 A:ASP714 4.6 0.6 1.0
CG A:ASP714 4.6 0.6 1.0
O A:GLY213 4.7 0.8 1.0
CG2 A:THR371 4.7 0.3 1.0
C A:GLY213 4.7 0.8 1.0
N A:ASP710 4.8 0.7 1.0
C A:ASP710 4.8 0.7 1.0
N A:GLY372 4.8 1.0 1.0
CB A:ASP710 4.9 0.6 1.0
ND2 A:ASN713 5.0 0.8 1.0
C A:GLY711 5.0 0.0 1.0

Magnesium binding site 2 out of 2 in 3n23

Go back to Magnesium Binding Sites List in 3n23
Magnesium binding site 2 out of 2 in the Crystal Structure of the High Affinity Complex Between Ouabain and the E2P Form of the Sodium-Potassium Pump


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of the High Affinity Complex Between Ouabain and the E2P Form of the Sodium-Potassium Pump within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Mg1017

b:0.7
occ:1.00
OP1 C:PHD369 2.5 0.9 1.0
O C:THR371 2.6 0.4 1.0
OD2 C:ASP710 2.7 0.3 1.0
OD2 C:PHD369 3.0 0.9 1.0
CG C:PHD369 3.4 0.9 1.0
OD1 C:PHD369 3.6 0.9 1.0
P C:PHD369 3.6 0.9 1.0
C C:THR371 3.6 0.4 1.0
CG C:ASP710 3.7 0.3 1.0
CA C:GLY213 3.7 0.9 1.0
CB C:THR371 3.8 0.9 1.0
N C:GLY711 3.8 0.8 1.0
O C:SER209 3.9 1.0 1.0
OD1 C:ASP710 3.9 0.3 1.0
CA C:GLY711 4.0 0.8 1.0
CA C:THR371 4.1 0.4 1.0
OD2 C:ASP714 4.2 0.1 1.0
OP2 C:PHD369 4.3 0.9 1.0
CG2 C:THR371 4.3 0.9 1.0
N C:THR371 4.3 0.4 1.0
O C:GLY213 4.4 0.9 1.0
C C:GLY213 4.5 0.9 1.0
OD1 C:ASN713 4.5 0.6 1.0
N C:GLY372 4.6 0.1 1.0
OP3 C:PHD369 4.8 0.9 1.0
CB C:PHD369 4.8 0.9 1.0
C C:SER209 4.8 1.0 1.0
OG1 C:THR371 4.9 0.9 1.0
N C:GLY213 4.9 0.9 1.0
C C:ASP710 4.9 0.7 1.0
OD1 C:ASP714 5.0 0.1 1.0

Reference:

L.Yatime, M.Laursen, J.P.Morth, M.Esmann, P.Nissen, N.U.Fedosova. Structural Insights Into the High Affinity Binding of Cardiotonic Steroids to the Na+,K+-Atpase. J.Struct.Biol. V. 174 296 2011.
ISSN: ISSN 1047-8477
PubMed: 21182963
DOI: 10.1016/J.JSB.2010.12.004
Page generated: Wed Aug 14 19:34:17 2024

Last articles

Mg in 4Y52
Mg in 4Y30
Mg in 4Y2V
Mg in 4Y2X
Mg in 4Y2Y
Mg in 4Y2U
Mg in 4Y2T
Mg in 4Y2Q
Mg in 4Y2R
Mg in 4Y2S
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy