Magnesium in PDB 3nbz: Crystal Structure of the Hiv-1 Rev Nes-CRM1-Rangtp Nuclear Export Complex (Crystal I)

The structure of Crystal Structure of the Hiv-1 Rev Nes-CRM1-Rangtp Nuclear Export Complex (Crystal I), PDB code: 3nbz was solved by T.Guttler, T.Madl, P.Neumann, D.Deichsel, L.Corsini, T.Monecke, R.Ficner, M.Sattler, D.Gorlich, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	38.90 / 2.80
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	72.660, 224.617, 164.021, 90.00, 100.82, 90.00
R / Rfree (%)	22.6 / 28.5

The structure of Crystal Structure of the Hiv-1 Rev Nes-CRM1-Rangtp Nuclear Export Complex (Crystal I) also contains other interesting chemical elements:

Sodium

(Na)

2 atoms

The binding sites of Magnesium atom in the Crystal Structure of the Hiv-1 Rev Nes-CRM1-Rangtp Nuclear Export Complex (Crystal I) (pdb code 3nbz). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of the Hiv-1 Rev Nes-CRM1-Rangtp Nuclear Export Complex (Crystal I), PDB code: 3nbz:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of the Hiv-1 Rev Nes-CRM1-Rangtp Nuclear Export Complex (Crystal I)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of the Hiv-1 Rev Nes-CRM1-Rangtp Nuclear Export Complex (Crystal I) within 5.0Å range: probe atom residue distance (Å) B Occ C:Mg218 b:14.5 occ:1.00 O3G C:GTP217 2.2 14.2 1.0 O2B C:GTP217 2.3 23.2 1.0 O C:HOH183 2.6 11.5 1.0 OH C:TYR39 2.8 24.8 1.0 CE1 C:TYR39 3.2 34.3 1.0 O C:HOH395 3.3 29.7 1.0 CZ C:TYR39 3.4 34.9 1.0 CB C:ALA41 3.5 14.7 1.0 PG C:GTP217 3.6 71.7 1.0 CA C:GLY19 3.6 30.9 1.0 PB C:GTP217 3.7 32.8 1.0 CA C:ALA41 3.8 14.7 1.0 N C:GLY20 3.8 30.3 1.0 O2G C:GTP217 4.1 14.1 1.0 N C:THR42 4.2 27.5 1.0 O3B C:GTP217 4.2 25.3 1.0 C C:GLY19 4.3 39.2 1.0 O1B C:GTP217 4.3 56.2 1.0 CG C:LEU69 4.3 46.3 1.0 N C:GLY68 4.5 20.4 1.0 C C:ALA41 4.5 30.0 1.0 CD1 C:TYR39 4.5 22.1 1.0 N C:LEU69 4.6 28.6 1.0 O1G C:GTP217 4.7 19.9 1.0 CD1 C:LEU69 4.7 64.4 1.0 O3A C:GTP217 4.8 21.5 1.0 CE2 C:TYR39 4.8 38.0 1.0 N C:ALA41 4.8 21.7 1.0 N C:GLY19 4.8 36.6 1.0 CB C:LEU69 4.9 31.6 1.0 CA C:GLY68 5.0 26.7 1.0 CA C:GLY20 5.0 25.8 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of the Hiv-1 Rev Nes-CRM1-Rangtp Nuclear Export Complex (Crystal I)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of the Hiv-1 Rev Nes-CRM1-Rangtp Nuclear Export Complex (Crystal I) within 5.0Å range: probe atom residue distance (Å) B Occ F:Mg218 b:26.0 occ:1.00 OG1 F:THR24 2.5 24.4 1.0 OG1 F:THR42 2.6 31.1 1.0 O1B F:GTP217 2.6 21.9 1.0 OD2 F:ASP65 2.7 37.0 1.0 O1G F:GTP217 2.8 56.0 1.0 CB F:THR42 3.2 33.7 1.0 O F:THR66 3.3 30.2 1.0 OD1 F:ASP65 3.4 47.1 1.0 CG F:ASP65 3.4 41.2 1.0 O3G F:GTP217 3.7 49.7 1.0 PG F:GTP217 3.7 47.2 1.0 CB F:THR24 3.8 37.9 1.0 PB F:GTP217 3.8 34.5 1.0 CG2 F:THR42 4.0 37.0 1.0 N F:THR24 4.1 34.7 1.0 O3B F:GTP217 4.2 27.8 1.0 CE F:LYS23 4.2 25.8 1.0 C F:THR66 4.3 35.5 1.0 CB F:LYS23 4.3 24.6 1.0 O2B F:GTP217 4.4 50.5 1.0 O F:ASP65 4.4 37.9 1.0 CA F:THR24 4.4 30.1 1.0 N F:THR42 4.4 25.0 1.0 CA F:THR42 4.4 34.3 1.0 CA F:ALA67 4.6 35.6 1.0 NZ F:LYS23 4.7 46.0 1.0 C F:ASP65 4.7 19.5 1.0 CB F:ASP65 4.8 11.6 1.0 N F:ALA67 4.9 39.9 1.0 CG2 F:THR24 4.9 36.1 1.0 CD F:LYS23 4.9 20.8 1.0 C F:LYS23 5.0 32.7 1.0

T.Guttler, T.Madl, P.Neumann, D.Deichsel, L.Corsini, T.Monecke, R.Ficner, M.Sattler, D.Gorlich. Nes Consensus Redefined By Structures of Pki-Type and Rev-Type Nuclear Export Signals Bound to CRM1. Nat.Struct.Mol.Biol. V. 17 1367 2010.
ISSN: ISSN 1545-9993
PubMed: 20972448
DOI: 10.1038/NSMB.1931