Magnesium in PDB 3ovb: How the Cca-Adding Enzyme Selects Adenine Over Cytosine in Position 76 of Trna

All present enzymatic activity of How the Cca-Adding Enzyme Selects Adenine Over Cytosine in Position 76 of Trna:
2.7.7.21; 2.7.7.25;

The structure of How the Cca-Adding Enzyme Selects Adenine Over Cytosine in Position 76 of Trna, PDB code: 3ovb was solved by B.C.Pan, Y.Xiong, T.A.Steitz, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	46.63 / 1.95
Space group	P 21 21 2
Cell size a, b, c (Å), α, β, γ (°)	110.978, 215.826, 58.390, 90.00, 90.00, 90.00
R / Rfree (%)	21.1 / 24.8

The binding sites of Magnesium atom in the How the Cca-Adding Enzyme Selects Adenine Over Cytosine in Position 76 of Trna (pdb code 3ovb). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the How the Cca-Adding Enzyme Selects Adenine Over Cytosine in Position 76 of Trna, PDB code: 3ovb:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the How the Cca-Adding Enzyme Selects Adenine Over Cytosine in Position 76 of Trna


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of How the Cca-Adding Enzyme Selects Adenine Over Cytosine in Position 76 of Trna within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg901 b:39.7 occ:1.00 O3G A:ATP501 2.7 23.9 0.5 OP1 C:A35 2.8 22.1 0.5 OE1 A:GLU59 2.9 41.2 1.0 O A:GLU59 3.1 30.0 1.0 O4 C:POP601 3.1 64.3 0.5 O1A A:ATP501 3.1 19.6 0.5 O2B A:ATP501 3.2 12.6 0.5 O A:HOH724 3.2 45.1 1.0 OD1 A:ASP61 3.2 25.2 1.0 O C:HOH478 3.2 40.4 1.0 O C:POP601 3.4 64.6 0.5 O A:HOH485 3.4 19.9 1.0 CD A:GLU59 3.5 40.9 1.0 CB A:GLU59 3.5 31.8 1.0 CG A:GLU59 3.8 36.2 1.0 C A:GLU59 3.8 30.1 1.0 P2 C:POP601 3.9 64.4 0.5 OG A:SER47 3.9 26.5 1.0 CG A:ASP61 3.9 26.7 1.0 O A:HOH565 4.0 35.5 1.0 OD2 A:ASP61 4.1 26.5 1.0 O3A A:ATP501 4.1 17.4 0.5 PB A:ATP501 4.1 14.1 0.5 PG A:ATP501 4.1 25.2 0.5 O3 C:POP601 4.2 64.9 0.5 PA A:ATP501 4.2 18.1 0.5 CA A:GLU59 4.2 31.8 1.0 P1 C:POP601 4.2 65.0 0.5 P C:A35 4.3 21.5 0.5 OE2 A:GLU59 4.4 43.2 1.0 O3B A:ATP501 4.5 19.8 0.5 O2 C:POP601 4.5 64.9 0.5 O5 C:POP601 4.6 64.9 0.5 N A:GLU59 4.7 32.3 1.0 N A:ILE60 4.8 28.8 1.0 O2G A:ATP501 4.8 23.9 0.5 N A:SER47 4.9 23.3 1.0 C5' C:A35 4.9 15.3 0.5

Magnesium binding site 2 out of 2 in the How the Cca-Adding Enzyme Selects Adenine Over Cytosine in Position 76 of Trna


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of How the Cca-Adding Enzyme Selects Adenine Over Cytosine in Position 76 of Trna within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg902 b:62.1 occ:1.00 OP1 D:A35 2.4 55.4 0.5 O2B B:ATP502 2.5 53.4 0.5 O3G B:ATP502 2.7 55.9 0.5 OD1 B:ASP61 2.8 58.6 1.0 O1A B:ATP502 2.9 53.0 0.5 O B:GLU59 3.3 68.2 1.0 PB B:ATP502 3.5 53.5 0.5 O2 B:POP602 3.6 65.0 0.5 CG B:ASP61 3.7 58.8 1.0 O3A B:ATP502 3.7 53.6 0.5 CB B:GLU59 3.8 65.3 1.0 O B:POP602 3.8 64.9 0.5 OE2 B:GLU59 3.8 58.4 1.0 P D:A35 3.9 55.6 0.5 OG B:SER47 3.9 69.4 1.0 OD2 B:ASP61 3.9 58.4 1.0 PA B:ATP502 3.9 52.7 0.5 C B:GLU59 4.0 68.1 1.0 PG B:ATP502 4.0 55.6 0.5 O3B B:ATP502 4.1 54.4 0.5 CD B:GLU59 4.1 60.5 1.0 CG B:GLU59 4.2 62.8 1.0 P1 B:POP602 4.4 64.9 0.5 CA B:GLU59 4.4 68.8 1.0 C5' D:A35 4.6 56.9 0.5 O2G B:ATP502 4.7 55.9 0.5 O5' D:A35 4.7 55.8 0.5 O3' D:C34 4.7 54.7 0.5 O3' D:C34 4.7 54.7 0.5 OP2 D:A35 4.7 55.6 0.5 O4 B:POP602 4.8 64.9 0.5 N B:SER47 4.9 69.2 1.0 N B:ILE60 4.9 68.2 1.0 O5' B:ATP502 4.9 52.7 0.5 N B:GLU59 4.9 71.9 1.0 O1B B:ATP502 4.9 53.3 0.5 OE1 B:GLU59 4.9 60.6 1.0 CB B:ASP61 4.9 61.1 1.0 C5' B:ATP502 4.9 52.1 0.5 P2 B:POP602 5.0 64.9 0.5

B.Pan, Y.Xiong, T.A.Steitz. How the Cca-Adding Enzyme Selects Adenine Over Cytosine at Position 76 of Trna. Science V. 330 937 2010.
ISSN: ISSN 0036-8075
PubMed: 21071662
DOI: 10.1126/SCIENCE.1194985