Magnesium in PDB 3pnp: The High Resolution Crystal Structure of Bovine Spleen Purine Nucleoside Phosphorylase in Complex Forms with Phosphate and 9- Deazainosine

Enzymatic activity of The High Resolution Crystal Structure of Bovine Spleen Purine Nucleoside Phosphorylase in Complex Forms with Phosphate and 9- Deazainosine

All present enzymatic activity of The High Resolution Crystal Structure of Bovine Spleen Purine Nucleoside Phosphorylase in Complex Forms with Phosphate and 9- Deazainosine:
2.4.2.1;

Protein crystallography data

The structure of The High Resolution Crystal Structure of Bovine Spleen Purine Nucleoside Phosphorylase in Complex Forms with Phosphate and 9- Deazainosine, PDB code: 3pnp was solved by M.J.Pugmire, C.Mao, S.E.Ealick, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

*Resolution Low / High (Å)*	8.00 / 1.60
*Space group*	P 2₁ 3
*Cell size a, b, c (Å), α, β, γ (°)*	93.000, 93.000, 93.000, 90.00, 90.00, 90.00
*R / R_free (%)*	20.8 / 23.2

Magnesium Binding Sites:

The binding sites of Magnesium atom in the The High Resolution Crystal Structure of Bovine Spleen Purine Nucleoside Phosphorylase in Complex Forms with Phosphate and 9- Deazainosine (pdb code 3pnp). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the The High Resolution Crystal Structure of Bovine Spleen Purine Nucleoside Phosphorylase in Complex Forms with Phosphate and 9- Deazainosine, PDB code: 3pnp:

Magnesium binding site 1 out of 1 in 3pnp

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Magnesium Binding Sites List in 3pnp

Magnesium binding site 1 out of 1 in the The High Resolution Crystal Structure of Bovine Spleen Purine Nucleoside Phosphorylase in Complex Forms with Phosphate and 9- Deazainosine

Mono view

Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of The High Resolution Crystal Structure of Bovine Spleen Purine Nucleoside Phosphorylase in Complex Forms with Phosphate and 9- Deazainosine within 5.0Å range:

probe	atom	residue	distance (Å)	B	Occ
A:Mg291 b:17.4 occ:1.00	O	A:HOH353	2.1	13.5	1.0
	O	A:HOH360	2.1	17.1	1.0
	O	A:HOH350	2.2	24.0	1.0
	O	A:HOH354	2.2	15.6	1.0
	O	A:HOH355	2.2	14.9	1.0
	O	A:HOH351	2.2	19.2	1.0
	OE2	A:GLU22	4.0	19.9	1.0
	OE1	A:GLU22	4.3	17.3	1.0
	O	A:GLN44	4.3	19.3	1.0
	O	A:HOH352	4.3	30.6	1.0
	OE1	A:GLN46	4.4	13.7	1.0
	O	A:ALA45	4.4	16.8	1.0
	CD	A:GLU22	4.5	17.8	1.0
	CB	A:GLN46	4.7	14.4	1.0
	C	A:GLN44	4.8	19.2	1.0

Reference:

M.J.Pugmire, C.Mao, S.E.Ealick. The High Resolution Crystal Structure of Bovine Spleen Purine Nucleoside Phosphorylase in Complex Forms with Phosphate and 9-Deazainosine To Be Published.

Page generated: Thu Aug 15 09:39:59 2024

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