Magnesium in PDB 3ref: Crystal Structure of EHRHO1 Bound to Gdp and Magnesium

The structure of Crystal Structure of EHRHO1 Bound to Gdp and Magnesium, PDB code: 3ref was solved by D.E.Bosch, C.Qiu, D.P.Siderovski, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	28.32 / 1.95
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	50.300, 54.476, 132.285, 90.00, 90.00, 90.00
R / Rfree (%)	15.7 / 19.7

The binding sites of Magnesium atom in the Crystal Structure of EHRHO1 Bound to Gdp and Magnesium (pdb code 3ref). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of EHRHO1 Bound to Gdp and Magnesium, PDB code: 3ref:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of EHRHO1 Bound to Gdp and Magnesium


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of EHRHO1 Bound to Gdp and Magnesium within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg550 b:21.8 occ:1.00 O3B B:GDP274 2.0 16.6 1.0 O B:HOH220 2.2 18.2 1.0 OG1 B:THR34 2.2 16.6 1.0 O B:HOH222 2.2 15.0 1.0 O B:HOH221 2.2 17.2 1.0 O B:THR52 2.3 18.6 1.0 CB B:THR34 3.2 12.6 1.0 PB B:GDP274 3.2 17.7 1.0 C B:THR52 3.4 24.4 1.0 O2B B:GDP274 3.4 18.7 1.0 N B:THR34 3.9 15.4 1.0 O B:PRO51 4.0 22.2 1.0 CA B:THR52 4.1 21.7 1.0 OD2 B:ASP74 4.1 21.2 1.0 O1A B:GDP274 4.1 18.1 1.0 CA B:THR34 4.1 14.7 1.0 OD1 B:ASP74 4.2 16.8 1.0 O1B B:GDP274 4.2 17.5 1.0 CG2 B:THR34 4.3 16.6 1.0 O3A B:GDP274 4.3 16.0 1.0 O B:HOH200 4.4 17.7 1.0 N B:VAL53 4.4 20.5 1.0 PA B:GDP274 4.5 16.3 1.0 O B:THR75 4.5 20.1 1.0 CG B:ASP74 4.6 20.5 1.0 CA B:VAL53 4.6 23.2 1.0 O2A B:GDP274 4.7 18.0 1.0 CG2 B:THR52 4.7 29.9 1.0 O B:HOH280 4.8 22.9 1.0 CG2 B:VAL53 4.8 41.2 1.0 NZ B:LYS33 4.8 17.8 1.0 CB B:LYS33 4.9 15.9 1.0 CE B:LYS33 4.9 13.9 1.0 C B:LYS33 5.0 19.9 1.0 C B:PRO51 5.0 23.0 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of EHRHO1 Bound to Gdp and Magnesium


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of EHRHO1 Bound to Gdp and Magnesium within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg192 b:30.0 occ:1.00 O A:HOH205 2.1 35.1 1.0 O A:HOH207 2.1 24.2 1.0 O A:THR52 2.3 28.7 1.0 O3B A:GDP274 2.3 17.1 1.0 O A:HOH256 2.3 31.4 1.0 OG1 A:THR34 2.3 25.6 1.0 PB A:GDP274 3.3 19.4 1.0 O2B A:GDP274 3.4 18.6 1.0 C A:THR52 3.4 34.0 1.0 CB A:THR34 3.4 23.3 1.0 OD2 A:ASP74 4.1 25.3 1.0 O1A A:GDP274 4.1 23.9 1.0 O A:PRO51 4.1 35.3 1.0 O A:HOH197 4.1 25.0 1.0 N A:THR34 4.1 19.6 1.0 CA A:THR52 4.2 42.1 1.0 OD1 A:ASP74 4.4 24.8 1.0 CA A:THR34 4.4 23.2 1.0 O3A A:GDP274 4.4 20.7 1.0 O1B A:GDP274 4.4 16.5 1.0 N A:VAL53 4.5 31.0 1.0 CG2 A:THR34 4.5 21.6 1.0 O A:THR75 4.6 18.6 1.0 CG A:ASP74 4.6 26.0 1.0 PA A:GDP274 4.6 22.6 1.0 CA A:VAL53 4.7 31.2 1.0 O2A A:GDP274 4.8 18.8 1.0 CG2 A:THR52 4.9 35.5 1.0 O A:HOH201 5.0 33.0 1.0 CB A:ALA76 5.0 18.6 1.0

D.E.Bosch, E.S.Wittchen, C.Qiu, K.Burridge, D.P.Siderovski. Unique Structural and Nucleotide Exchange Features of the RHO1 Gtpase of Entamoeba Histolytica. J.Biol.Chem. V. 286 39236 2011.
ISSN: ISSN 0021-9258
PubMed: 21930699
DOI: 10.1074/JBC.M111.253898