Magnesium in PDB 3rlg: Crystal Structure of Loxosceles Intermedia Phospholipase D Isoform 1 H12A Mutant

All present enzymatic activity of Crystal Structure of Loxosceles Intermedia Phospholipase D Isoform 1 H12A Mutant:
3.1.4.41;

The structure of Crystal Structure of Loxosceles Intermedia Phospholipase D Isoform 1 H12A Mutant, PDB code: 3rlg was solved by P.O.Giuseppe, A.Ullah, S.S.Veiga, M.T.Murakami, R.K.Arni, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	27.16 / 1.60
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	49.575, 49.455, 56.395, 90.00, 105.56, 90.00
R / Rfree (%)	16.4 / 19.8

The binding sites of Magnesium atom in the Crystal Structure of Loxosceles Intermedia Phospholipase D Isoform 1 H12A Mutant (pdb code 3rlg). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the Crystal Structure of Loxosceles Intermedia Phospholipase D Isoform 1 H12A Mutant, PDB code: 3rlg:

Magnesium binding site 1 out of 1 in the Crystal Structure of Loxosceles Intermedia Phospholipase D Isoform 1 H12A Mutant


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of Loxosceles Intermedia Phospholipase D Isoform 1 H12A Mutant within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg286 b:21.4 occ:1.00 OE2 A:GLU32 2.2 26.2 1.0 OD1 A:ASP34 2.2 23.1 1.0 OD2 A:ASP91 2.3 25.0 1.0 O A:HOH475 2.3 33.2 1.0 O A:HOH357 2.3 25.4 1.0 CD A:GLU32 3.2 22.9 1.0 CG A:ASP91 3.3 21.5 1.0 CG A:ASP34 3.3 21.2 1.0 OE1 A:GLU32 3.5 21.1 1.0 CB A:ASP91 3.5 18.1 1.0 OD2 A:ASP34 3.7 22.6 1.0 O A:HOH470 3.9 46.5 1.0 NZ A:LYS93 3.9 33.7 1.0 CD2 A:HIS47 4.1 29.8 1.0 CE A:LYS93 4.1 29.1 1.0 OD1 A:ASP91 4.4 22.3 1.0 CG A:GLU32 4.5 19.4 1.0 NE2 A:HIS47 4.6 30.6 1.0 CB A:ASP34 4.6 19.1 1.0 CA A:ASP34 5.0 19.3 1.0

A.Ullah, P.O.De Giuseppe, M.T.Murakami, D.Trevisan-Silva, A.C.Wille, D.Chaves-Moreira, L.H.Gremski, R.B.Da Silveira, A.Sennf-Ribeiro, O.M.Chaim, S.S.Veiga, R.K.Arni. Crystallization and Preliminary X-Ray Diffraction Analysis of A Class II Phospholipase D From Loxosceles Intermedia Venom. Acta Crystallogr.,Sect.F V. 67 234 2011.
ISSN: ESSN 1744-3091
PubMed: 21301094
DOI: 10.1107/S1744309110050931