Magnesium in PDB 3sbd: Crystal Structure of RAC1 P29S Mutant

The structure of Crystal Structure of RAC1 P29S Mutant, PDB code: 3sbd was solved by B.H.Ha, T.J.Boggon, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.68 / 2.10
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	50.266, 80.021, 94.910, 90.00, 90.00, 90.00
R / Rfree (%)	23.8 / 28.5

The binding sites of Magnesium atom in the Crystal Structure of RAC1 P29S Mutant (pdb code 3sbd). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of RAC1 P29S Mutant, PDB code: 3sbd:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of RAC1 P29S Mutant


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of RAC1 P29S Mutant within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg501 b:26.6 occ:1.00 O2G A:GNP500 1.9 23.6 1.0 O2B A:GNP500 2.1 22.1 1.0 O A:HOH233 2.1 19.7 1.0 O A:HOH234 2.1 19.9 1.0 OG1 A:THR35 2.1 28.8 1.0 OG1 A:THR17 2.1 24.4 1.0 PG A:GNP500 3.1 25.5 1.0 CB A:THR35 3.1 28.1 1.0 PB A:GNP500 3.2 24.1 1.0 CB A:THR17 3.3 24.1 1.0 N3B A:GNP500 3.3 23.2 1.0 N A:THR35 3.7 28.8 1.0 O1G A:GNP500 3.9 27.4 1.0 N A:THR17 3.9 23.5 1.0 OD2 A:ASP57 4.0 28.6 1.0 O2A A:GNP500 4.0 24.3 1.0 CA A:THR35 4.0 28.6 1.0 CA A:THR17 4.2 24.3 1.0 O1B A:GNP500 4.2 25.3 1.0 OD1 A:ASP57 4.2 24.8 1.0 O3A A:GNP500 4.2 24.7 1.0 CG2 A:THR35 4.2 27.1 1.0 O3G A:GNP500 4.3 22.2 1.0 CG2 A:THR17 4.3 24.0 1.0 PA A:GNP500 4.4 25.8 1.0 CG A:ASP57 4.5 27.9 1.0 O A:ILE33 4.5 33.1 1.0 O A:THR58 4.6 26.9 1.0 O1A A:GNP500 4.7 24.9 1.0 C A:PRO34 4.7 29.9 1.0 CB A:LYS16 4.7 21.8 1.0 CE A:LYS16 5.0 20.8 1.0 CA A:PRO34 5.0 30.7 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of RAC1 P29S Mutant


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of RAC1 P29S Mutant within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg503 b:22.4 occ:1.00 O3G B:GNP502 1.9 20.7 1.0 O2B B:GNP502 2.1 24.4 1.0 O B:HOH235 2.1 19.0 1.0 OG1 B:THR35 2.1 28.2 1.0 OG1 B:THR17 2.2 21.9 1.0 O B:HOH236 2.2 23.6 1.0 PG B:GNP502 3.1 24.4 1.0 CB B:THR35 3.1 27.9 1.0 PB B:GNP502 3.1 24.0 1.0 N3B B:GNP502 3.2 24.0 1.0 CB B:THR17 3.3 23.6 1.0 N B:THR35 3.8 29.0 1.0 O2G B:GNP502 3.9 24.7 1.0 N B:THR17 4.0 23.3 1.0 CA B:THR35 4.0 28.6 1.0 OD2 B:ASP57 4.1 27.2 1.0 O2A B:GNP502 4.1 24.8 1.0 O3A B:GNP502 4.1 25.4 1.0 OD1 B:ASP57 4.2 24.4 1.0 O1G B:GNP502 4.2 23.7 1.0 O1B B:GNP502 4.2 24.6 1.0 CA B:THR17 4.2 23.4 1.0 CG2 B:THR35 4.2 27.2 1.0 CG2 B:THR17 4.3 22.6 1.0 PA B:GNP502 4.5 27.7 1.0 CG B:ASP57 4.5 27.0 1.0 O B:ILE33 4.6 31.5 1.0 O B:HOH237 4.6 41.5 1.0 O B:THR58 4.6 26.4 1.0 O1A B:GNP502 4.7 26.6 1.0 C B:PRO34 4.8 30.2 1.0 CB B:LYS16 4.9 22.5 1.0 CE B:LYS16 4.9 18.9 1.0

M.Krauthammer, Y.Kong, B.H.Ha, P.Evans, A.Bacchiocchi, J.P.Mccusker, E.Cheng, M.J.Davis, G.Goh, M.Choi, S.Ariyan, D.Narayan, K.Dutton-Regester, A.Capatana, E.C.Holman, M.Bosenberg, M.Sznol, H.M.Kluger, D.E.Brash, D.F.Stern, M.A.Materin, R.S.Lo, S.Mane, S.Ma, K.K.Kidd, N.K.Hayward, R.P.Lifton, J.Schlessinger, T.J.Boggon, R.Halaban. Exome Sequencing Identifies Recurrent Somatic RAC1 Mutations in Melanoma. Nat.Genet. V. 44 1006 2012.
ISSN: ISSN 1061-4036
PubMed: 22842228
DOI: 10.1038/NG.2359