Magnesium in PDB 3sjd: Crystal Structure of S. Cerevisiae GET3 with Bound Adp-MG2+ in Complex with GET2 Cytosolic Domain

The structure of Crystal Structure of S. Cerevisiae GET3 with Bound Adp-MG2+ in Complex with GET2 Cytosolic Domain, PDB code: 3sjd was solved by S.Reitz, K.Wild, I.Sinning, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	56.23 / 4.60
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	149.640, 209.750, 133.240, 90.00, 90.00, 90.00
R / Rfree (%)	32.3 / 38.8

The structure of Crystal Structure of S. Cerevisiae GET3 with Bound Adp-MG2+ in Complex with GET2 Cytosolic Domain also contains other interesting chemical elements:

Zinc

(Zn)

2 atoms

The binding sites of Magnesium atom in the Crystal Structure of S. Cerevisiae GET3 with Bound Adp-MG2+ in Complex with GET2 Cytosolic Domain (pdb code 3sjd). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 3 binding sites of Magnesium where determined in the Crystal Structure of S. Cerevisiae GET3 with Bound Adp-MG2+ in Complex with GET2 Cytosolic Domain, PDB code: 3sjd:
Jump to Magnesium binding site number: 1; 2; 3;

Magnesium binding site 1 out of 3 in the Crystal Structure of S. Cerevisiae GET3 with Bound Adp-MG2+ in Complex with GET2 Cytosolic Domain


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of S. Cerevisiae GET3 with Bound Adp-MG2+ in Complex with GET2 Cytosolic Domain within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg402 b:0.1 occ:1.00 OG1 A:THR32 2.0 0.8 1.0 O2B A:ADP401 2.1 0.0 1.0 CG2 A:THR32 2.3 0.6 1.0 CB A:THR32 2.5 0.2 1.0 ND2 A:ASN61 2.9 0.9 1.0 CA A:THR32 3.2 0.5 1.0 PB A:ADP401 3.4 0.5 1.0 O1B A:ADP401 3.5 0.1 1.0 N A:THR32 3.6 0.9 1.0 NZ A:LYS31 3.7 0.7 1.0 CE A:LYS31 3.8 0.5 1.0 OD2 A:ASP166 3.9 0.7 1.0 CG A:ASN61 3.9 0.8 1.0 O2A A:ADP401 4.2 0.1 1.0 CB A:ASN61 4.3 0.7 1.0 O3A A:ADP401 4.3 0.0 1.0 O3B A:ADP401 4.4 0.1 1.0 OD1 A:ASP166 4.5 0.0 1.0 OD2 A:ASP57 4.6 0.7 1.0 C A:THR32 4.6 0.5 1.0 C A:LYS31 4.7 0.3 1.0 CG A:ASP166 4.7 0.2 1.0 PA A:ADP401 4.7 0.6 1.0 O1A A:ADP401 4.8 0.8 1.0 OD1 A:ASN61 5.0 0.9 1.0

Magnesium binding site 2 out of 3 in the Crystal Structure of S. Cerevisiae GET3 with Bound Adp-MG2+ in Complex with GET2 Cytosolic Domain


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of S. Cerevisiae GET3 with Bound Adp-MG2+ in Complex with GET2 Cytosolic Domain within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg402 b:0.6 occ:1.00 O2B B:ADP401 2.1 0.5 1.0 OG1 B:THR32 2.2 0.5 1.0 CG2 B:THR32 2.4 0.2 1.0 CB B:THR32 2.6 0.3 1.0 ND2 B:ASN61 2.8 0.3 1.0 CA B:THR32 3.3 0.9 1.0 O1B B:ADP401 3.3 0.1 1.0 PB B:ADP401 3.4 0.5 1.0 NZ B:LYS31 3.7 0.5 1.0 N B:THR32 3.7 0.5 1.0 CE B:LYS31 3.9 0.1 1.0 OD2 B:ASP166 3.9 0.3 1.0 CG B:ASN61 3.9 0.4 1.0 O2A B:ADP401 4.2 0.8 1.0 O3A B:ADP401 4.2 0.7 1.0 CB B:ASN61 4.2 0.3 1.0 O3B B:ADP401 4.3 0.6 1.0 OD2 B:ASP57 4.6 0.8 1.0 OD1 B:ASP166 4.6 0.5 1.0 PA B:ADP401 4.7 0.7 1.0 CG B:ASP166 4.7 0.6 1.0 C B:THR32 4.7 0.2 1.0 C B:LYS31 4.8 0.3 1.0 O1A B:ADP401 4.9 0.1 1.0 OD1 B:ASN61 5.0 0.5 1.0

Magnesium binding site 3 out of 3 in the Crystal Structure of S. Cerevisiae GET3 with Bound Adp-MG2+ in Complex with GET2 Cytosolic Domain


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Crystal Structure of S. Cerevisiae GET3 with Bound Adp-MG2+ in Complex with GET2 Cytosolic Domain within 5.0Å range: probe atom residue distance (Å) B Occ C:Mg402 b:0.6 occ:1.00 O2B C:ADP401 2.1 0.3 1.0 OG1 C:THR32 2.2 0.5 1.0 CG2 C:THR32 2.4 0.4 1.0 CB C:THR32 2.6 0.7 1.0 ND2 C:ASN61 2.9 0.0 1.0 CA C:THR32 3.3 0.9 1.0 PB C:ADP401 3.4 0.0 1.0 O1B C:ADP401 3.4 0.7 1.0 NZ C:LYS31 3.6 0.4 1.0 N C:THR32 3.7 0.5 1.0 CE C:LYS31 3.8 0.3 1.0 OD2 C:ASP166 3.9 0.7 1.0 CG C:ASN61 4.0 0.2 1.0 O2A C:ADP401 4.2 0.1 1.0 O3A C:ADP401 4.3 0.2 1.0 CB C:ASN61 4.3 0.0 1.0 O3B C:ADP401 4.3 0.5 1.0 OD2 C:ASP57 4.6 0.8 1.0 OD1 C:ASP166 4.6 0.2 1.0 PA C:ADP401 4.7 0.5 1.0 C C:THR32 4.7 0.4 1.0 C C:LYS31 4.7 0.2 1.0 CG C:ASP166 4.7 0.3 1.0 O1A C:ADP401 4.8 0.4 1.0

S.Stefer, S.Reitz, F.Wang, K.Wild, Y.Y.Pang, D.Schwarz, J.Bomke, C.Hein, F.Lohr, F.Bernhard, V.Denic, V.Dotsch, I.Sinning. Structural Basis For Tail-Anchored Membrane Protein Biogenesis By the GET3-Receptor Complex. Science V. 333 758 2011.
ISSN: ISSN 0036-8075
PubMed: 21719644
DOI: 10.1126/SCIENCE.1207125