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Magnesium in PDB 3sm4: Crystal Structure of the K131A Mutant of Lambda Exonuclease in Complex with A 5'-Phosphorylated 14-Mer/12-Mer Duplex and Magnesium
Enzymatic activity of Crystal Structure of the K131A Mutant of Lambda Exonuclease in Complex with A 5'-Phosphorylated 14-Mer/12-Mer Duplex and Magnesium
All present enzymatic activity of Crystal Structure of the K131A Mutant of Lambda Exonuclease in Complex with A 5'-Phosphorylated 14-Mer/12-Mer Duplex and Magnesium:
3.1.11.3;
3.1.11.3;
Protein crystallography data
The structure of Crystal Structure of the K131A Mutant of Lambda Exonuclease in Complex with A 5'-Phosphorylated 14-Mer/12-Mer Duplex and Magnesium, PDB code: 3sm4
was solved by
C.E.Bell,
J.Zhang,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
| Resolution Low / High (Å) | 67.89 / 1.88 |
| Space group | P 65 |
| Cell size a, b, c (Å), α, β, γ (°) | 78.391, 78.391, 247.534, 90.00, 90.00, 120.00 |
| R / Rfree (%) | 16 / 18.6 |
Other elements in 3sm4:
The structure of Crystal Structure of the K131A Mutant of Lambda Exonuclease in Complex with A 5'-Phosphorylated 14-Mer/12-Mer Duplex and Magnesium also contains other interesting chemical elements:
| Chlorine | (Cl) | 2 atoms |
Magnesium Binding Sites:
The binding sites of Magnesium atom in the Crystal Structure of the K131A Mutant of Lambda Exonuclease in Complex with A 5'-Phosphorylated 14-Mer/12-Mer Duplex and Magnesium
(pdb code 3sm4). This binding sites where shown within
5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of the K131A Mutant of Lambda Exonuclease in Complex with A 5'-Phosphorylated 14-Mer/12-Mer Duplex and Magnesium, PDB code: 3sm4:
Jump to Magnesium binding site number: 1; 2;
In total 2 binding sites of Magnesium where determined in the Crystal Structure of the K131A Mutant of Lambda Exonuclease in Complex with A 5'-Phosphorylated 14-Mer/12-Mer Duplex and Magnesium, PDB code: 3sm4:
Jump to Magnesium binding site number: 1; 2;
Magnesium binding site 1 out of 2 in 3sm4
Go back to
Magnesium Binding Sites List in 3sm4
Magnesium binding site 1 out
of 2 in the Crystal Structure of the K131A Mutant of Lambda Exonuclease in Complex with A 5'-Phosphorylated 14-Mer/12-Mer Duplex and Magnesium
Mono view
Stereo pair view
Mono view
Stereo pair view
A full contact list of Magnesium with other atoms in the Mg binding
site number 1 of Crystal Structure of the K131A Mutant of Lambda Exonuclease in Complex with A 5'-Phosphorylated 14-Mer/12-Mer Duplex and Magnesium within 5.0Å range:
|
Magnesium binding site 2 out of 2 in 3sm4
Go back to
Magnesium Binding Sites List in 3sm4
Magnesium binding site 2 out
of 2 in the Crystal Structure of the K131A Mutant of Lambda Exonuclease in Complex with A 5'-Phosphorylated 14-Mer/12-Mer Duplex and Magnesium
Mono view
Stereo pair view
Mono view
Stereo pair view
A full contact list of Magnesium with other atoms in the Mg binding
site number 2 of Crystal Structure of the K131A Mutant of Lambda Exonuclease in Complex with A 5'-Phosphorylated 14-Mer/12-Mer Duplex and Magnesium within 5.0Å range:
|
Reference:
J.Zhang,
K.A.Mccabe,
C.E.Bell.
Crystal Structures of {Lambda} Exonuclease in Complex with Dna Suggest An Electrostatic Ratchet Mechanism For Processivity. Proc.Natl.Acad.Sci.Usa V. 108 11872 2011.
ISSN: ISSN 0027-8424
PubMed: 21730170
DOI: 10.1073/PNAS.1103467108
Page generated: Thu Aug 15 11:04:12 2024
ISSN: ISSN 0027-8424
PubMed: 21730170
DOI: 10.1073/PNAS.1103467108
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