Magnesium in PDB 3t0a: E. Coli (Lacz) Beta-Galactosidase (S796T)
Enzymatic activity of E. Coli (Lacz) Beta-Galactosidase (S796T)
All present enzymatic activity of E. Coli (Lacz) Beta-Galactosidase (S796T):
3.2.1.23;
Protein crystallography data
The structure of E. Coli (Lacz) Beta-Galactosidase (S796T), PDB code: 3t0a
was solved by
L.J.Jancewicz,
R.W.Wheatley,
G.Sutendra,
M.Lee,
M.Fraser,
R.E.Huber,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
|
Resolution Low / High (Å)
|
61.72 /
1.90
|
|
Space group
|
P 21 21 21
|
|
Cell size a, b, c (Å), α, β, γ (°)
|
150.920,
161.220,
202.900,
90.00,
90.00,
90.00
|
|
R / Rfree (%)
|
17 /
21.4
|
Other elements in 3t0a:
The structure of E. Coli (Lacz) Beta-Galactosidase (S796T) also contains other interesting chemical elements:
Magnesium Binding Sites:
Pages:
>>> Page 1 <<<
Page 2, Binding sites: 11 -
12;
Binding sites:
The binding sites of Magnesium atom in the E. Coli (Lacz) Beta-Galactosidase (S796T)
(pdb code 3t0a). This binding sites where shown within
5.0 Angstroms radius around Magnesium atom.
In total 12 binding sites of Magnesium where determined in the
E. Coli (Lacz) Beta-Galactosidase (S796T), PDB code: 3t0a:
Jump to Magnesium binding site number:
1;
2;
3;
4;
5;
6;
7;
8;
9;
10;
Magnesium binding site 1 out
of 12 in 3t0a
Go back to
Magnesium Binding Sites List in 3t0a
Magnesium binding site 1 out
of 12 in the E. Coli (Lacz) Beta-Galactosidase (S796T)
 Mono view
 Stereo pair view
|
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 1 of E. Coli (Lacz) Beta-Galactosidase (S796T) within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Mg3001
b:29.5
occ:1.00
|
ND1
|
A:HIS418
|
2.1
|
25.6
|
1.0
|
|
OE2
|
A:GLU416
|
2.1
|
31.5
|
1.0
|
|
OE1
|
A:GLU461
|
2.1
|
24.4
|
1.0
|
|
O
|
A:HOH4843
|
2.1
|
24.7
|
1.0
|
|
O
|
A:HOH4688
|
2.2
|
25.8
|
1.0
|
|
O
|
A:HOH4751
|
2.3
|
23.9
|
1.0
|
|
CE1
|
A:HIS418
|
2.9
|
29.4
|
1.0
|
|
CD
|
A:GLU461
|
3.1
|
32.7
|
1.0
|
|
CG
|
A:HIS418
|
3.2
|
27.1
|
1.0
|
|
CD
|
A:GLU416
|
3.2
|
25.3
|
1.0
|
|
CB
|
A:HIS418
|
3.6
|
21.8
|
1.0
|
|
OE1
|
A:GLU416
|
3.8
|
25.7
|
1.0
|
|
OE2
|
A:GLU461
|
3.8
|
29.2
|
1.0
|
|
O
|
A:HOH4388
|
4.0
|
32.7
|
1.0
|
|
CB
|
A:GLU461
|
4.1
|
23.5
|
1.0
|
|
NE2
|
A:HIS418
|
4.1
|
24.3
|
1.0
|
|
CG
|
A:GLU461
|
4.1
|
24.5
|
1.0
|
|
OD1
|
A:ASN102
|
4.1
|
30.9
|
1.0
|
|
O
|
A:HOH4238
|
4.2
|
36.1
|
1.0
|
|
CD2
|
A:HIS418
|
4.2
|
26.7
|
1.0
|
|
CB
|
A:ASP201
|
4.2
|
29.9
|
1.0
|
|
N
|
A:ASP201
|
4.2
|
29.8
|
1.0
|
|
O
|
A:ASP199
|
4.3
|
28.9
|
1.0
|
|
ND2
|
A:ASN460
|
4.3
|
23.1
|
1.0
|
|
CG
|
A:GLU416
|
4.4
|
23.3
|
1.0
|
|
O
|
A:HOH4240
|
4.5
|
44.8
|
1.0
|
|
O
|
A:ASN102
|
4.6
|
33.4
|
1.0
|
|
CA
|
A:ASP201
|
4.8
|
29.7
|
1.0
|
|
CG2
|
A:VAL103
|
4.9
|
30.4
|
1.0
|
|
Magnesium binding site 2 out
of 12 in 3t0a
Go back to
Magnesium Binding Sites List in 3t0a
Magnesium binding site 2 out
of 12 in the E. Coli (Lacz) Beta-Galactosidase (S796T)
 Mono view
 Stereo pair view
|
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 2 of E. Coli (Lacz) Beta-Galactosidase (S796T) within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Mg3002
b:31.4
occ:1.00
|
OD2
|
A:ASP193
|
2.1
|
41.9
|
1.0
|
|
O
|
A:ASP15
|
2.1
|
33.7
|
1.0
|
|
O
|
A:ASN18
|
2.3
|
31.6
|
1.0
|
|
OE1
|
A:GLN163
|
2.3
|
31.9
|
1.0
|
|
O
|
A:VAL21
|
2.3
|
34.9
|
1.0
|
|
CG
|
A:ASP193
|
2.9
|
41.4
|
1.0
|
|
OD1
|
A:ASP193
|
3.0
|
37.4
|
1.0
|
|
CD
|
A:GLN163
|
3.2
|
34.0
|
1.0
|
|
C
|
A:ASN18
|
3.2
|
32.1
|
1.0
|
|
C
|
A:ASP15
|
3.3
|
36.7
|
1.0
|
|
C
|
A:VAL21
|
3.5
|
33.2
|
1.0
|
|
NE2
|
A:GLN163
|
3.6
|
30.1
|
1.0
|
|
N
|
A:ASN18
|
3.6
|
35.1
|
1.0
|
|
CA
|
A:ASN18
|
3.9
|
34.7
|
1.0
|
|
OH
|
A:TYR161
|
3.9
|
34.5
|
1.0
|
|
CA
|
A:TRP16
|
4.0
|
35.7
|
1.0
|
|
N
|
A:TRP16
|
4.1
|
36.6
|
1.0
|
|
C
|
A:TRP16
|
4.2
|
34.9
|
1.0
|
|
CB
|
A:ASN18
|
4.2
|
32.2
|
1.0
|
|
N
|
A:PRO19
|
4.2
|
34.0
|
1.0
|
|
CA
|
A:VAL21
|
4.2
|
32.8
|
1.0
|
|
CE2
|
A:TYR161
|
4.3
|
32.7
|
1.0
|
|
CB
|
A:VAL21
|
4.3
|
34.6
|
1.0
|
|
CB
|
A:ASP193
|
4.3
|
32.6
|
1.0
|
|
N
|
A:GLU17
|
4.4
|
34.9
|
1.0
|
|
N
|
A:VAL21
|
4.4
|
34.6
|
1.0
|
|
CA
|
A:ASP15
|
4.4
|
38.7
|
1.0
|
|
CG
|
A:GLN163
|
4.5
|
31.9
|
1.0
|
|
N
|
A:THR22
|
4.5
|
31.5
|
1.0
|
|
CA
|
A:PRO19
|
4.5
|
34.1
|
1.0
|
|
CZ
|
A:TYR161
|
4.6
|
32.6
|
1.0
|
|
CA
|
A:THR22
|
4.6
|
33.5
|
1.0
|
|
O
|
A:TRP16
|
4.7
|
36.1
|
1.0
|
|
CG1
|
A:VAL21
|
4.8
|
27.9
|
1.0
|
|
C
|
A:GLU17
|
4.8
|
36.4
|
1.0
|
|
CB
|
A:ASP15
|
4.8
|
38.5
|
1.0
|
|
C
|
A:PRO19
|
5.0
|
35.5
|
1.0
|
|
Magnesium binding site 3 out
of 12 in 3t0a
Go back to
Magnesium Binding Sites List in 3t0a
Magnesium binding site 3 out
of 12 in the E. Coli (Lacz) Beta-Galactosidase (S796T)
 Mono view
 Stereo pair view
|
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 3 of E. Coli (Lacz) Beta-Galactosidase (S796T) within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Mg3003
b:54.9
occ:1.00
|
O
|
A:HOH4816
|
2.1
|
36.9
|
1.0
|
|
O
|
A:HOH4789
|
2.2
|
44.5
|
1.0
|
|
O
|
A:HOH4578
|
2.2
|
23.0
|
0.5
|
|
OE1
|
A:GLN718
|
2.3
|
40.3
|
1.0
|
|
O
|
A:HOH4459
|
2.4
|
44.6
|
1.0
|
|
O
|
A:HOH4334
|
2.7
|
46.6
|
1.0
|
|
CD
|
A:GLN718
|
3.3
|
30.8
|
1.0
|
|
NE2
|
A:GLN718
|
3.7
|
27.8
|
1.0
|
|
O
|
A:THR911
|
4.0
|
24.2
|
1.0
|
|
NE2
|
A:HIS622
|
4.1
|
22.6
|
1.0
|
|
O
|
A:HOH4321
|
4.3
|
36.5
|
1.0
|
|
O
|
A:HOH4380
|
4.3
|
52.8
|
1.0
|
|
O
|
A:HOH4540
|
4.3
|
35.5
|
1.0
|
|
O
|
A:HOH4438
|
4.4
|
23.8
|
1.0
|
|
O
|
A:GLN719
|
4.6
|
24.1
|
1.0
|
|
CG
|
A:GLN718
|
4.6
|
25.1
|
1.0
|
|
CE1
|
A:HIS622
|
4.7
|
24.2
|
1.0
|
|
N
|
A:GLN719
|
4.7
|
21.4
|
1.0
|
|
O
|
A:HOH4835
|
4.8
|
43.3
|
1.0
|
|
O
|
A:HOH4208
|
4.8
|
29.4
|
1.0
|
|
O
|
A:HOH4342
|
4.9
|
42.8
|
1.0
|
|
C
|
A:THR911
|
4.9
|
21.6
|
1.0
|
|
CB
|
A:THR911
|
5.0
|
22.3
|
1.0
|
|
Magnesium binding site 4 out
of 12 in 3t0a
Go back to
Magnesium Binding Sites List in 3t0a
Magnesium binding site 4 out
of 12 in the E. Coli (Lacz) Beta-Galactosidase (S796T)
 Mono view
 Stereo pair view
|
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 4 of E. Coli (Lacz) Beta-Galactosidase (S796T) within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Mg3001
b:23.9
occ:1.00
|
ND1
|
B:HIS418
|
2.1
|
19.6
|
1.0
|
|
OE2
|
B:GLU416
|
2.1
|
20.7
|
1.0
|
|
OE1
|
B:GLU461
|
2.1
|
22.4
|
1.0
|
|
O
|
B:HOH4872
|
2.1
|
19.1
|
1.0
|
|
O
|
B:HOH4943
|
2.1
|
20.9
|
1.0
|
|
O
|
B:HOH4979
|
2.3
|
20.6
|
1.0
|
|
CE1
|
B:HIS418
|
2.9
|
24.9
|
1.0
|
|
CG
|
B:HIS418
|
3.1
|
21.4
|
1.0
|
|
CD
|
B:GLU461
|
3.2
|
30.8
|
1.0
|
|
CD
|
B:GLU416
|
3.2
|
17.6
|
1.0
|
|
CB
|
B:HIS418
|
3.6
|
19.7
|
1.0
|
|
OE1
|
B:GLU416
|
3.8
|
20.1
|
1.0
|
|
OE2
|
B:GLU461
|
3.9
|
21.8
|
1.0
|
|
CB
|
B:GLU461
|
4.1
|
18.7
|
1.0
|
|
NE2
|
B:HIS418
|
4.1
|
25.0
|
1.0
|
|
OD1
|
B:ASN102
|
4.1
|
27.3
|
1.0
|
|
CG
|
B:GLU461
|
4.2
|
20.8
|
1.0
|
|
O
|
B:HOH4264
|
4.2
|
37.8
|
1.0
|
|
CD2
|
B:HIS418
|
4.2
|
20.5
|
1.0
|
|
O
|
B:HOH4017
|
4.2
|
25.0
|
1.0
|
|
O
|
B:ASP199
|
4.3
|
21.7
|
1.0
|
|
CB
|
B:ASP201
|
4.3
|
23.0
|
1.0
|
|
ND2
|
B:ASN460
|
4.3
|
20.9
|
1.0
|
|
N
|
B:ASP201
|
4.3
|
21.9
|
1.0
|
|
CG
|
B:GLU416
|
4.4
|
18.3
|
1.0
|
|
O
|
B:HOH4277
|
4.6
|
35.2
|
1.0
|
|
O
|
B:ASN102
|
4.6
|
26.7
|
1.0
|
|
CA
|
B:ASP201
|
4.9
|
21.2
|
1.0
|
|
CG2
|
B:VAL103
|
4.9
|
23.6
|
1.0
|
|
Magnesium binding site 5 out
of 12 in 3t0a
Go back to
Magnesium Binding Sites List in 3t0a
Magnesium binding site 5 out
of 12 in the E. Coli (Lacz) Beta-Galactosidase (S796T)
 Mono view
 Stereo pair view
|
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 5 of E. Coli (Lacz) Beta-Galactosidase (S796T) within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Mg3002
b:25.0
occ:1.00
|
OD2
|
B:ASP193
|
2.1
|
29.5
|
1.0
|
|
O
|
B:ASP15
|
2.1
|
22.2
|
1.0
|
|
O
|
B:ASN18
|
2.2
|
23.9
|
1.0
|
|
OE1
|
B:GLN163
|
2.3
|
27.3
|
1.0
|
|
O
|
B:VAL21
|
2.3
|
25.6
|
1.0
|
|
CG
|
B:ASP193
|
2.9
|
27.0
|
1.0
|
|
OD1
|
B:ASP193
|
3.0
|
32.0
|
1.0
|
|
C
|
B:ASN18
|
3.2
|
24.9
|
1.0
|
|
CD
|
B:GLN163
|
3.2
|
32.9
|
1.0
|
|
C
|
B:ASP15
|
3.3
|
23.1
|
1.0
|
|
C
|
B:VAL21
|
3.5
|
23.9
|
1.0
|
|
NE2
|
B:GLN163
|
3.6
|
26.3
|
1.0
|
|
N
|
B:ASN18
|
3.6
|
26.6
|
1.0
|
|
CA
|
B:ASN18
|
3.8
|
26.0
|
1.0
|
|
OH
|
B:TYR161
|
3.9
|
20.1
|
1.0
|
|
CA
|
B:TRP16
|
4.0
|
26.6
|
1.0
|
|
N
|
B:TRP16
|
4.1
|
23.7
|
1.0
|
|
N
|
B:PRO19
|
4.1
|
26.4
|
1.0
|
|
C
|
B:TRP16
|
4.2
|
26.9
|
1.0
|
|
CB
|
B:ASN18
|
4.2
|
26.1
|
1.0
|
|
CA
|
B:VAL21
|
4.2
|
26.8
|
1.0
|
|
CB
|
B:VAL21
|
4.3
|
28.6
|
1.0
|
|
CE2
|
B:TYR161
|
4.3
|
25.1
|
1.0
|
|
CB
|
B:ASP193
|
4.3
|
23.5
|
1.0
|
|
N
|
B:GLU17
|
4.4
|
26.7
|
1.0
|
|
N
|
B:VAL21
|
4.4
|
28.5
|
1.0
|
|
CA
|
B:PRO19
|
4.4
|
27.9
|
1.0
|
|
CA
|
B:ASP15
|
4.4
|
26.2
|
1.0
|
|
N
|
B:THR22
|
4.5
|
25.8
|
1.0
|
|
CG
|
B:GLN163
|
4.5
|
23.9
|
1.0
|
|
CZ
|
B:TYR161
|
4.6
|
27.9
|
1.0
|
|
CA
|
B:THR22
|
4.7
|
26.3
|
1.0
|
|
C
|
B:GLU17
|
4.8
|
28.5
|
1.0
|
|
O
|
B:TRP16
|
4.8
|
28.8
|
1.0
|
|
CG1
|
B:VAL21
|
4.8
|
27.1
|
1.0
|
|
CB
|
B:ASP15
|
4.9
|
24.3
|
1.0
|
|
C
|
B:PRO19
|
4.9
|
28.2
|
1.0
|
|
Magnesium binding site 6 out
of 12 in 3t0a
Go back to
Magnesium Binding Sites List in 3t0a
Magnesium binding site 6 out
of 12 in the E. Coli (Lacz) Beta-Galactosidase (S796T)
 Mono view
 Stereo pair view
|
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 6 of E. Coli (Lacz) Beta-Galactosidase (S796T) within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Mg3003
b:50.2
occ:1.00
|
O
|
B:HOH4697
|
1.9
|
22.5
|
0.5
|
|
O
|
B:HOH4253
|
2.3
|
34.0
|
1.0
|
|
O
|
B:HOH4322
|
2.4
|
38.1
|
1.0
|
|
O
|
B:HOH4971
|
2.5
|
48.5
|
1.0
|
|
OE1
|
B:GLN718
|
2.6
|
32.4
|
1.0
|
|
O
|
B:HOH4333
|
2.7
|
40.1
|
1.0
|
|
CD
|
B:GLN718
|
3.6
|
27.2
|
1.0
|
|
O
|
B:THR911
|
3.7
|
22.9
|
1.0
|
|
NE2
|
B:GLN718
|
3.9
|
25.4
|
1.0
|
|
C1
|
B:DMS8019
|
4.1
|
68.8
|
1.0
|
|
NE2
|
B:HIS622
|
4.1
|
17.6
|
1.0
|
|
O
|
B:HOH4321
|
4.1
|
35.0
|
1.0
|
|
O
|
B:HOH4559
|
4.2
|
47.6
|
1.0
|
|
O
|
B:HOH4699
|
4.2
|
42.4
|
1.0
|
|
O
|
B:HOH4391
|
4.3
|
31.3
|
1.0
|
|
O
|
B:HOH4092
|
4.5
|
23.2
|
1.0
|
|
O
|
B:HOH4380
|
4.6
|
39.8
|
1.0
|
|
C
|
B:THR911
|
4.6
|
21.9
|
1.0
|
|
CA
|
B:THR911
|
4.7
|
20.9
|
1.0
|
|
CB
|
B:THR911
|
4.7
|
19.6
|
1.0
|
|
O
|
B:DMS8019
|
4.8
|
69.3
|
1.0
|
|
O
|
B:GLN719
|
4.8
|
22.6
|
1.0
|
|
CE1
|
B:HIS622
|
4.8
|
18.7
|
1.0
|
|
N
|
B:GLN719
|
4.9
|
21.6
|
1.0
|
|
CG
|
B:GLN718
|
5.0
|
21.7
|
1.0
|
|
S
|
B:DMS8019
|
5.0
|
69.6
|
1.0
|
|
Magnesium binding site 7 out
of 12 in 3t0a
Go back to
Magnesium Binding Sites List in 3t0a
Magnesium binding site 7 out
of 12 in the E. Coli (Lacz) Beta-Galactosidase (S796T)
 Mono view
 Stereo pair view
|
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 7 of E. Coli (Lacz) Beta-Galactosidase (S796T) within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
C:Mg3001
b:23.2
occ:1.00
|
ND1
|
C:HIS418
|
2.1
|
20.8
|
1.0
|
|
O
|
C:HOH4958
|
2.1
|
19.2
|
1.0
|
|
OE2
|
C:GLU416
|
2.1
|
23.1
|
1.0
|
|
OE1
|
C:GLU461
|
2.1
|
22.6
|
1.0
|
|
O
|
C:HOH4920
|
2.1
|
18.8
|
1.0
|
|
O
|
C:HOH4989
|
2.2
|
21.2
|
1.0
|
|
CE1
|
C:HIS418
|
2.9
|
23.0
|
1.0
|
|
CG
|
C:HIS418
|
3.1
|
19.7
|
1.0
|
|
CD
|
C:GLU461
|
3.2
|
27.6
|
1.0
|
|
CD
|
C:GLU416
|
3.2
|
21.9
|
1.0
|
|
CB
|
C:HIS418
|
3.6
|
20.2
|
1.0
|
|
OE1
|
C:GLU416
|
3.7
|
20.7
|
1.0
|
|
OE2
|
C:GLU461
|
3.9
|
28.1
|
1.0
|
|
CB
|
C:GLU461
|
4.1
|
19.6
|
1.0
|
|
NE2
|
C:HIS418
|
4.1
|
23.7
|
1.0
|
|
OD1
|
C:ASN102
|
4.2
|
27.0
|
1.0
|
|
CG
|
C:GLU461
|
4.2
|
18.3
|
1.0
|
|
CD2
|
C:HIS418
|
4.2
|
22.1
|
1.0
|
|
CB
|
C:ASP201
|
4.2
|
19.0
|
1.0
|
|
O
|
C:ASP199
|
4.2
|
22.8
|
1.0
|
|
O
|
C:HOH4279
|
4.3
|
25.8
|
1.0
|
|
N
|
C:ASP201
|
4.3
|
20.8
|
1.0
|
|
O
|
C:HOH4482
|
4.3
|
40.0
|
1.0
|
|
ND2
|
C:ASN460
|
4.3
|
21.6
|
1.0
|
|
CG
|
C:GLU416
|
4.4
|
17.0
|
1.0
|
|
O
|
C:HOH4023
|
4.5
|
39.2
|
1.0
|
|
O
|
C:ASN102
|
4.6
|
27.4
|
1.0
|
|
CA
|
C:ASP201
|
4.8
|
21.6
|
1.0
|
|
CG2
|
C:VAL103
|
4.8
|
25.8
|
1.0
|
|
C
|
C:GLN200
|
5.0
|
24.2
|
1.0
|
|
CA
|
C:GLN200
|
5.0
|
22.3
|
1.0
|
|
Magnesium binding site 8 out
of 12 in 3t0a
Go back to
Magnesium Binding Sites List in 3t0a
Magnesium binding site 8 out
of 12 in the E. Coli (Lacz) Beta-Galactosidase (S796T)
 Mono view
 Stereo pair view
|
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 8 of E. Coli (Lacz) Beta-Galactosidase (S796T) within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
C:Mg3002
b:21.8
occ:1.00
|
O
|
C:ASP15
|
2.1
|
25.0
|
1.0
|
|
OD2
|
C:ASP193
|
2.1
|
25.8
|
1.0
|
|
OE1
|
C:GLN163
|
2.3
|
25.9
|
1.0
|
|
O
|
C:ASN18
|
2.3
|
24.4
|
1.0
|
|
O
|
C:VAL21
|
2.3
|
25.7
|
1.0
|
|
CG
|
C:ASP193
|
2.9
|
25.6
|
1.0
|
|
OD1
|
C:ASP193
|
3.0
|
24.7
|
1.0
|
|
CD
|
C:GLN163
|
3.2
|
27.4
|
1.0
|
|
C
|
C:ASN18
|
3.2
|
22.0
|
1.0
|
|
C
|
C:ASP15
|
3.3
|
25.6
|
1.0
|
|
C
|
C:VAL21
|
3.4
|
28.1
|
1.0
|
|
NE2
|
C:GLN163
|
3.5
|
24.9
|
1.0
|
|
N
|
C:ASN18
|
3.7
|
27.8
|
1.0
|
|
CA
|
C:ASN18
|
3.9
|
25.2
|
1.0
|
|
OH
|
C:TYR161
|
3.9
|
22.6
|
1.0
|
|
CA
|
C:TRP16
|
4.0
|
26.2
|
1.0
|
|
N
|
C:TRP16
|
4.1
|
24.0
|
1.0
|
|
CA
|
C:VAL21
|
4.2
|
31.2
|
1.0
|
|
C
|
C:TRP16
|
4.2
|
26.1
|
1.0
|
|
N
|
C:PRO19
|
4.2
|
24.0
|
1.0
|
|
CB
|
C:VAL21
|
4.3
|
31.6
|
1.0
|
|
N
|
C:VAL21
|
4.3
|
32.3
|
1.0
|
|
CB
|
C:ASP193
|
4.3
|
24.6
|
1.0
|
|
CB
|
C:ASN18
|
4.3
|
23.9
|
1.0
|
|
CE2
|
C:TYR161
|
4.3
|
21.1
|
1.0
|
|
N
|
C:GLU17
|
4.4
|
23.7
|
1.0
|
|
CA
|
C:ASP15
|
4.4
|
25.3
|
1.0
|
|
N
|
C:THR22
|
4.4
|
23.6
|
1.0
|
|
CA
|
C:PRO19
|
4.5
|
24.2
|
1.0
|
|
CG
|
C:GLN163
|
4.5
|
23.1
|
1.0
|
|
CZ
|
C:TYR161
|
4.6
|
24.2
|
1.0
|
|
CG1
|
C:VAL21
|
4.7
|
29.4
|
1.0
|
|
CA
|
C:THR22
|
4.7
|
24.9
|
1.0
|
|
O
|
C:TRP16
|
4.7
|
27.6
|
1.0
|
|
C
|
C:GLU17
|
4.8
|
26.7
|
1.0
|
|
CB
|
C:ASP15
|
4.9
|
22.4
|
1.0
|
|
C
|
C:PRO19
|
4.9
|
28.0
|
1.0
|
|
Magnesium binding site 9 out
of 12 in 3t0a
Go back to
Magnesium Binding Sites List in 3t0a
Magnesium binding site 9 out
of 12 in the E. Coli (Lacz) Beta-Galactosidase (S796T)
 Mono view
 Stereo pair view
|
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 9 of E. Coli (Lacz) Beta-Galactosidase (S796T) within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
C:Mg3003
b:39.0
occ:0.50
|
O
|
C:HOH4265
|
2.1
|
30.2
|
1.0
|
|
O
|
C:HOH4531
|
2.2
|
40.4
|
1.0
|
|
OE1
|
C:GLN718
|
2.3
|
34.7
|
1.0
|
|
O
|
C:HOH4530
|
2.5
|
44.6
|
1.0
|
|
CD
|
C:GLN718
|
3.3
|
30.7
|
1.0
|
|
O
|
C:HOH4812
|
3.6
|
41.4
|
1.0
|
|
NE2
|
C:GLN718
|
3.7
|
29.4
|
1.0
|
|
O
|
C:HOH4569
|
3.8
|
57.2
|
1.0
|
|
O
|
C:THR911
|
3.9
|
21.4
|
1.0
|
|
NE2
|
C:HIS622
|
4.0
|
20.0
|
1.0
|
|
O
|
C:HOH4708
|
4.2
|
45.8
|
1.0
|
|
O
|
C:DMS8017
|
4.4
|
78.6
|
1.0
|
|
N
|
C:GLN719
|
4.5
|
20.9
|
1.0
|
|
O
|
C:GLN719
|
4.5
|
22.2
|
1.0
|
|
C1
|
C:DMS8017
|
4.5
|
77.5
|
1.0
|
|
CE1
|
C:HIS622
|
4.6
|
21.5
|
1.0
|
|
CG
|
C:GLN718
|
4.6
|
20.3
|
1.0
|
|
O
|
C:HOH4258
|
4.7
|
28.7
|
1.0
|
|
O
|
C:HOH4097
|
4.7
|
22.8
|
1.0
|
|
O
|
C:HOH4245
|
4.8
|
44.2
|
1.0
|
|
C
|
C:THR911
|
4.8
|
20.1
|
1.0
|
|
C2
|
C:DMS8017
|
4.9
|
76.8
|
1.0
|
|
S
|
C:DMS8017
|
5.0
|
76.9
|
1.0
|
|
Magnesium binding site 10 out
of 12 in 3t0a
Go back to
Magnesium Binding Sites List in 3t0a
Magnesium binding site 10 out
of 12 in the E. Coli (Lacz) Beta-Galactosidase (S796T)
 Mono view
 Stereo pair view
|
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 10 of E. Coli (Lacz) Beta-Galactosidase (S796T) within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
D:Mg3001
b:27.2
occ:1.00
|
ND1
|
D:HIS418
|
2.0
|
26.4
|
1.0
|
|
O
|
D:HOH4792
|
2.1
|
24.2
|
1.0
|
|
OE2
|
D:GLU416
|
2.1
|
25.4
|
1.0
|
|
OE1
|
D:GLU461
|
2.1
|
25.8
|
1.0
|
|
O
|
D:HOH4680
|
2.2
|
22.2
|
1.0
|
|
O
|
D:HOH4882
|
2.2
|
27.3
|
1.0
|
|
CE1
|
D:HIS418
|
2.9
|
29.2
|
1.0
|
|
CD
|
D:GLU461
|
3.1
|
31.4
|
1.0
|
|
CG
|
D:HIS418
|
3.1
|
22.5
|
1.0
|
|
CD
|
D:GLU416
|
3.2
|
26.5
|
1.0
|
|
CB
|
D:HIS418
|
3.6
|
21.6
|
1.0
|
|
OE1
|
D:GLU416
|
3.8
|
26.7
|
1.0
|
|
OE2
|
D:GLU461
|
3.8
|
32.5
|
1.0
|
|
O
|
D:HOH4430
|
4.0
|
39.3
|
1.0
|
|
NE2
|
D:HIS418
|
4.1
|
26.9
|
1.0
|
|
CB
|
D:GLU461
|
4.1
|
25.2
|
1.0
|
|
O
|
D:HOH4036
|
4.2
|
31.2
|
1.0
|
|
CG
|
D:GLU461
|
4.2
|
26.9
|
1.0
|
|
OD1
|
D:ASN102
|
4.2
|
31.3
|
1.0
|
|
CD2
|
D:HIS418
|
4.2
|
26.9
|
1.0
|
|
CB
|
D:ASP201
|
4.2
|
31.2
|
1.0
|
|
O
|
D:ASP199
|
4.3
|
31.9
|
1.0
|
|
ND2
|
D:ASN460
|
4.3
|
28.8
|
1.0
|
|
N
|
D:ASP201
|
4.3
|
29.4
|
1.0
|
|
CG
|
D:GLU416
|
4.4
|
27.4
|
1.0
|
|
O
|
D:HOH4280
|
4.6
|
42.1
|
1.0
|
|
O
|
D:ASN102
|
4.6
|
32.6
|
1.0
|
|
CA
|
D:ASP201
|
4.9
|
29.4
|
1.0
|
|
Reference:
L.J.Jancewicz,
R.W.Wheatley,
G.Sutendra,
M.Lee,
M.E.Fraser,
R.E.Huber.
Ser-796 of Beta-Galactosidase (E. Coli) Plays A Key Role in Maintaining An Optimum Balance Between the Opened and Closed Conformations of the Catalytically Important Active Site Loop Arch.Biochem.Biophys. V. 517 111 2012.
ISSN: ISSN 0003-9861
PubMed: 22155115
DOI: 10.1016/J.ABB.2011.11.017
Page generated: Thu Aug 15 11:12:44 2024
|
