Magnesium in PDB 3tm0: Crystal Structure of 3',5"-Aminoglycoside Phosphotransferase Type Iiia Amppnp Butirosin A Complex

All present enzymatic activity of Crystal Structure of 3',5"-Aminoglycoside Phosphotransferase Type Iiia Amppnp Butirosin A Complex:
2.7.1.95;

The structure of Crystal Structure of 3',5"-Aminoglycoside Phosphotransferase Type Iiia Amppnp Butirosin A Complex, PDB code: 3tm0 was solved by A.M.Berghuis, D.H.Fong, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	39.57 / 2.10
Space group	P 42 21 2
Cell size a, b, c (Å), α, β, γ (°)	80.112, 80.112, 110.600, 90.00, 90.00, 90.00
R / Rfree (%)	17.9 / 22.4

The binding sites of Magnesium atom in the Crystal Structure of 3',5"-Aminoglycoside Phosphotransferase Type Iiia Amppnp Butirosin A Complex (pdb code 3tm0). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of 3',5"-Aminoglycoside Phosphotransferase Type Iiia Amppnp Butirosin A Complex, PDB code: 3tm0:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of 3',5"-Aminoglycoside Phosphotransferase Type Iiia Amppnp Butirosin A Complex


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of 3',5"-Aminoglycoside Phosphotransferase Type Iiia Amppnp Butirosin A Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg265 b:28.4 occ:1.00 O1G A:ANP300 1.9 27.1 1.0 O2B A:ANP300 2.1 26.1 1.0 O A:HOH432 2.2 22.3 1.0 OD2 A:ASP208 2.2 20.9 1.0 O A:HOH400 2.2 20.6 1.0 OD1 A:ASP208 2.3 24.5 1.0 CG A:ASP208 2.5 26.1 1.0 PG A:ANP300 3.1 26.6 1.0 PB A:ANP300 3.4 23.2 1.0 O2G A:ANP300 3.5 26.9 1.0 N3B A:ANP300 3.7 21.2 1.0 MG A:MG266 3.8 24.5 1.0 O14 A:B31305 3.9 26.1 1.0 O A:HOH280 4.1 17.6 1.0 O A:HOH349 4.1 36.0 1.0 CB A:ASP208 4.1 17.1 1.0 OD2 A:ASP190 4.1 21.9 1.0 O A:HOH271 4.1 17.8 1.0 O A:HOH307 4.3 24.1 1.0 O2A A:ANP300 4.3 19.2 1.0 CB A:SER27 4.3 49.5 1.0 O3A A:ANP300 4.4 33.0 1.0 O3G A:ANP300 4.4 32.1 1.0 O1B A:ANP300 4.4 17.2 1.0 O A:HOH399 4.5 19.1 1.0 NZ A:LYS44 4.5 15.6 1.0 O13 A:B31305 4.6 24.6 1.0 OG A:SER27 4.6 43.1 1.0 PA A:ANP300 4.7 21.6 1.0 CA A:ASP208 4.9 18.6 1.0 ND2 A:ASN195 4.9 19.7 1.0 O1A A:ANP300 5.0 22.7 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of 3',5"-Aminoglycoside Phosphotransferase Type Iiia Amppnp Butirosin A Complex


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of 3',5"-Aminoglycoside Phosphotransferase Type Iiia Amppnp Butirosin A Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg266 b:24.5 occ:1.00 O2G A:ANP300 1.9 26.9 1.0 OD1 A:ASN195 2.0 24.1 1.0 O2A A:ANP300 2.0 19.2 1.0 O A:HOH399 2.2 19.1 1.0 OD2 A:ASP208 2.2 20.9 1.0 O A:HOH353 2.3 24.8 1.0 CG A:ASN195 2.9 25.4 1.0 CG A:ASP208 3.2 26.1 1.0 ND2 A:ASN195 3.2 19.7 1.0 PG A:ANP300 3.4 26.6 1.0 PA A:ANP300 3.5 21.6 1.0 CB A:ASP208 3.6 17.1 1.0 O1G A:ANP300 3.8 27.1 1.0 MG A:MG265 3.8 28.4 1.0 O2B A:ANP300 3.9 26.1 1.0 O3A A:ANP300 4.0 33.0 1.0 O A:HOH358 4.0 48.0 1.0 OD1 A:ASP208 4.3 24.5 1.0 PB A:ANP300 4.3 23.2 1.0 CB A:ASN195 4.3 20.5 1.0 N3B A:ANP300 4.3 21.2 1.0 O1A A:ANP300 4.3 22.7 1.0 O A:SER194 4.4 26.1 1.0 O A:HOH342 4.4 45.6 1.0 O3G A:ANP300 4.4 32.1 1.0 O13 A:B31305 4.4 24.6 1.0 O5' A:ANP300 4.5 20.4 1.0 O3' A:ANP300 4.6 23.8 1.0 C5' A:ANP300 4.6 26.1 1.0 OD2 A:ASP190 4.6 21.9 1.0 O A:HOH392 4.6 14.1 1.0 CA A:ASN195 4.7 17.4 1.0 C A:SER194 4.8 21.8 1.0 O A:HOH400 4.8 20.6 1.0 N A:ASN195 4.9 25.5 1.0 C3' A:ANP300 5.0 27.0 1.0

D.H.Fong, A.M.Berghuis. Structural Basis of Aph(3')-Iiia-Mediated Resistance to N1-Substituted Aminoglycoside Antibiotics. Antimicrob.Agents Chemother. V. 53 3049 2009.
ISSN: ISSN 0066-4804
PubMed: 19433564
DOI: 10.1128/AAC.00062-09