Magnesium in PDB 3u0o: The Crystal Structure of Selenophosphate Synthetase From E. Coli

All present enzymatic activity of The Crystal Structure of Selenophosphate Synthetase From E. Coli:
2.7.9.3;

The structure of The Crystal Structure of Selenophosphate Synthetase From E. Coli, PDB code: 3u0o was solved by N.Noinaj, R.Wattanasak, J.Wally, T.Stadtman, S.K.Buchanan, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	28.98 / 2.25
Space group	P 32 2 1
Cell size a, b, c (Å), α, β, γ (°)	102.347, 102.347, 140.669, 90.00, 90.00, 120.00
R / Rfree (%)	22.2 / 26.4

The binding sites of Magnesium atom in the The Crystal Structure of Selenophosphate Synthetase From E. Coli (pdb code 3u0o). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the The Crystal Structure of Selenophosphate Synthetase From E. Coli, PDB code: 3u0o:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the The Crystal Structure of Selenophosphate Synthetase From E. Coli


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of The Crystal Structure of Selenophosphate Synthetase From E. Coli within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg401 b:0.3 occ:1.00 OD1 A:ASP51 2.0 0.7 1.0 OD1 A:ASP227 2.0 70.6 1.0 OD1 A:ASP91 2.0 87.0 1.0 CG A:ASP227 2.9 73.6 1.0 CG A:ASP51 3.1 0.5 1.0 CG A:ASP91 3.1 75.0 1.0 OD2 A:ASP227 3.1 75.3 1.0 OD2 A:ASP91 3.6 82.9 1.0 CB A:ASP51 3.8 0.5 1.0 OG1 A:THR229 4.0 74.0 1.0 OD2 A:ASP51 4.1 91.2 1.0 O A:HOH531 4.2 79.1 1.0 O A:ASP227 4.2 55.7 1.0 CB A:ASP227 4.3 74.1 1.0 CB A:ASP91 4.4 73.2 1.0 CG2 A:THR229 4.6 59.3 1.0 N A:ASP227 4.6 52.9 1.0 CA A:ASP51 4.7 92.3 1.0 CA A:ASP91 4.8 65.4 1.0 CA A:ASP227 4.8 62.5 1.0 C A:ASP227 4.8 58.0 1.0 NH1 A:ARG49 4.9 0.1 1.0 CB A:THR229 4.9 79.9 1.0 NH2 A:ARG49 5.0 0.3 1.0

Magnesium binding site 2 out of 2 in the The Crystal Structure of Selenophosphate Synthetase From E. Coli


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of The Crystal Structure of Selenophosphate Synthetase From E. Coli within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg401 b:0.9 occ:1.00 OD1 B:ASP91 2.0 0.7 1.0 OD1 B:ASP227 2.0 0.3 1.0 CG B:ASP227 2.9 96.7 1.0 OD2 B:ASP227 3.0 0.3 1.0 CG B:ASP91 3.1 0.8 1.0 OD2 B:ASP91 3.6 0.8 1.0 OG1 B:THR229 3.6 75.1 1.0 CB B:ASP227 4.3 83.3 1.0 CB B:ASP91 4.3 86.1 1.0 CA B:ASP91 4.4 78.8 1.0 OD1 B:ASP51 4.7 0.7 1.0 OD2 B:ASP51 4.7 0.0 1.0 CG B:ASP51 4.7 0.9 1.0 N B:ASP91 4.7 69.1 1.0 CB B:THR229 4.8 70.5 1.0 CG2 B:THR229 4.8 55.8 1.0 O B:ASP227 4.9 70.8 1.0

N.Noinaj, R.Wattanasak, D.Y.Lee, J.L.Wally, G.Piszczek, P.B.Chock, T.C.Stadtman, S.K.Buchanan. Structural Insights Into the Catalytic Mechanism of Escherichia Coli Selenophosphate Synthetase. J.Bacteriol. V. 194 499 2012.
ISSN: ISSN 0021-9193
PubMed: 22081394
DOI: 10.1128/JB.06012-11