Magnesium in PDB 3ut5: Tubulin-Colchicine-Ustiloxin: Stathmin-Like Domain Complex

The structure of Tubulin-Colchicine-Ustiloxin: Stathmin-Like Domain Complex, PDB code: 3ut5 was solved by F.M.Ranaivoson, B.Gigant, M.Knossow, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	38.26 / 2.73
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	64.810, 128.870, 254.840, 90.00, 90.00, 90.00
R / Rfree (%)	18.4 / 22.2

The binding sites of Magnesium atom in the Tubulin-Colchicine-Ustiloxin: Stathmin-Like Domain Complex (pdb code 3ut5). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Tubulin-Colchicine-Ustiloxin: Stathmin-Like Domain Complex, PDB code: 3ut5:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Tubulin-Colchicine-Ustiloxin: Stathmin-Like Domain Complex


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Tubulin-Colchicine-Ustiloxin: Stathmin-Like Domain Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg502 b:74.6 occ:1.00 O2G A:GTP501 1.9 65.8 1.0 O A:HOH611 2.0 61.6 1.0 O A:HOH613 2.0 55.5 1.0 O A:HOH607 2.1 52.2 1.0 O1B A:GTP501 2.1 64.1 1.0 O B:HOH606 2.2 64.3 1.0 PB A:GTP501 2.9 64.5 1.0 PG A:GTP501 3.0 64.6 1.0 O3B A:GTP501 3.3 64.3 1.0 O3A A:GTP501 3.4 65.5 1.0 O1G A:GTP501 3.7 66.4 1.0 ND2 B:ASN249 4.0 0.9 1.0 NZ B:LYS254 4.0 88.8 1.0 CB A:GLN11 4.1 66.5 1.0 OD2 A:ASP69 4.2 76.2 1.0 O2A A:GTP501 4.3 67.5 1.0 N A:GLN11 4.3 64.6 1.0 O3G A:GTP501 4.3 66.2 1.0 PA A:GTP501 4.4 66.5 1.0 O2B A:GTP501 4.4 63.6 1.0 OD1 A:ASP69 4.4 72.7 1.0 OD2 A:ASP98 4.4 87.7 1.0 CB A:ASP98 4.6 71.2 1.0 CG A:ASP69 4.7 72.7 1.0 CB B:ASN249 4.8 0.8 1.0 CA A:GLN11 4.8 65.0 1.0 CG A:ASP98 4.9 78.9 1.0 CG B:ASN249 4.9 0.2 1.0 CE B:LYS254 4.9 84.3 1.0 O1A A:GTP501 4.9 66.5 1.0 OE1 A:GLU71 4.9 86.9 1.0

Magnesium binding site 2 out of 2 in the Tubulin-Colchicine-Ustiloxin: Stathmin-Like Domain Complex


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Tubulin-Colchicine-Ustiloxin: Stathmin-Like Domain Complex within 5.0Å range: probe atom residue distance (Å) B Occ C:Mg601 b:56.2 occ:1.00 O2G C:GTP600 1.9 59.5 1.0 O C:HOH713 2.1 43.6 1.0 O1B C:GTP600 2.1 58.0 1.0 O D:HOH622 2.2 65.3 1.0 O C:HOH714 2.3 43.6 1.0 O C:HOH725 2.3 57.9 1.0 PB C:GTP600 2.9 58.2 1.0 PG C:GTP600 3.0 58.4 1.0 O3B C:GTP600 3.2 57.7 1.0 O3A C:GTP600 3.4 57.9 1.0 O1G C:GTP600 3.7 60.8 1.0 OD1 D:ASN249 3.8 0.6 1.0 NZ D:LYS254 4.0 84.2 1.0 CB C:GLN11 4.1 51.1 1.0 OD2 C:ASP69 4.2 61.4 1.0 N C:GLN11 4.3 50.2 1.0 O2A C:GTP600 4.3 59.1 1.0 O3G C:GTP600 4.3 58.4 1.0 PA C:GTP600 4.4 58.8 1.0 O2B C:GTP600 4.4 58.0 1.0 OD1 C:ASP69 4.4 67.0 1.0 OD2 C:ASP98 4.5 81.7 1.0 CB C:ASP98 4.6 61.4 1.0 CG D:ASN249 4.7 0.8 1.0 CB D:ASN249 4.7 0.3 1.0 CG C:ASP69 4.7 64.2 1.0 CA C:GLN11 4.8 50.6 1.0 OE1 C:GLU71 4.9 71.4 1.0 CG C:ASP98 4.9 72.6 1.0 CE D:LYS254 4.9 80.0 1.0 O1A C:GTP600 5.0 59.0 1.0

F.M.Ranaivoson, B.Gigant, S.Berritt, M.Joullie, M.Knossow. Structural Plasticity of Tubulin Assembly Probed By Vinca-Domain Ligands. Acta Crystallogr.,Sect.D V. 68 927 2012.
ISSN: ISSN 0907-4449
PubMed: 22868758
DOI: 10.1107/S0907444912017143