Magnesium in PDB 3vhx: The Crystal Structure of ARF6-MKLP1 (Mitotic Kinesin-Like Protein 1) Complex

The structure of The Crystal Structure of ARF6-MKLP1 (Mitotic Kinesin-Like Protein 1) Complex, PDB code: 3vhx was solved by H.Makyio, T.Takei, H.Ohgi, S.Takahashi, H.Takatsu, T.Ueda, Y.Kanaho, Y.Xie, H.W.Shin, H.Kamikubo, M.Kataoka, M.Kawasaki, R.Kato, S.Wakatsuki, K.Nakayama, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.00 / 2.81
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	45.743, 174.573, 76.814, 90.00, 98.74, 90.00
R / Rfree (%)	20.1 / 23.2

The binding sites of Magnesium atom in the The Crystal Structure of ARF6-MKLP1 (Mitotic Kinesin-Like Protein 1) Complex (pdb code 3vhx). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 4 binding sites of Magnesium where determined in the The Crystal Structure of ARF6-MKLP1 (Mitotic Kinesin-Like Protein 1) Complex, PDB code: 3vhx:
Jump to Magnesium binding site number: 1; 2; 3; 4;

Magnesium binding site 1 out of 4 in the The Crystal Structure of ARF6-MKLP1 (Mitotic Kinesin-Like Protein 1) Complex


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of The Crystal Structure of ARF6-MKLP1 (Mitotic Kinesin-Like Protein 1) Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg185 b:44.3 occ:1.00 OG1 A:THR27 1.8 40.3 1.0 O1B A:GTP184 2.0 41.6 1.0 O A:HOH193 2.2 47.7 1.0 O1G A:GTP184 2.3 42.3 1.0 OG1 A:THR44 2.4 40.5 1.0 CB A:THR27 2.9 40.4 1.0 CB A:THR44 3.3 41.1 1.0 PB A:GTP184 3.4 41.9 1.0 PG A:GTP184 3.5 43.7 1.0 O3B A:GTP184 3.8 43.5 1.0 O1A A:GTP184 3.8 46.6 1.0 N A:THR27 3.8 38.7 1.0 CG2 A:THR27 3.9 40.9 1.0 OD2 A:ASP63 3.9 38.1 1.0 CA A:THR27 3.9 39.6 1.0 N A:THR44 3.9 44.5 1.0 O A:ILE42 4.0 47.3 1.0 CA A:THR44 4.2 43.3 1.0 O3G A:GTP184 4.2 43.0 1.0 O3A A:GTP184 4.3 44.3 1.0 PA A:GTP184 4.4 45.4 1.0 O2B A:GTP184 4.4 42.1 1.0 OD1 A:ASP63 4.4 37.9 1.0 O2A A:GTP184 4.4 43.7 1.0 CG2 A:THR44 4.5 41.1 1.0 CG A:ASP63 4.6 37.8 1.0 O2G A:GTP184 4.7 46.6 1.0 C A:PRO43 4.8 46.9 1.0 ND2 A:ASN48 4.9 41.7 1.0 C A:LYS26 5.0 38.1 1.0 CA A:PRO43 5.0 48.4 1.0

Magnesium binding site 2 out of 4 in the The Crystal Structure of ARF6-MKLP1 (Mitotic Kinesin-Like Protein 1) Complex


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of The Crystal Structure of ARF6-MKLP1 (Mitotic Kinesin-Like Protein 1) Complex within 5.0Å range: probe atom residue distance (Å) B Occ C:Mg185 b:25.9 occ:1.00 O C:HOH210 1.7 24.1 1.0 O1G C:GTP184 1.9 26.4 1.0 O1B C:GTP184 2.2 24.9 1.0 OG1 C:THR44 2.2 28.8 1.0 O C:HOH211 2.2 28.3 1.0 OG1 C:THR27 2.3 26.6 1.0 CB C:THR44 2.9 27.5 1.0 PG C:GTP184 3.1 25.5 1.0 CB C:THR27 3.4 27.9 1.0 PB C:GTP184 3.4 23.9 1.0 O3G C:GTP184 3.4 25.8 1.0 O3B C:GTP184 3.6 25.7 1.0 N C:THR44 3.6 30.2 1.0 OD2 C:ASP63 3.8 26.4 1.0 CA C:THR44 3.9 29.3 1.0 OD1 C:ASP63 4.1 24.6 1.0 CG2 C:THR44 4.1 26.5 1.0 N C:THR27 4.1 25.8 1.0 O2B C:GTP184 4.2 23.9 1.0 O C:ILE42 4.3 34.7 1.0 CA C:THR27 4.3 26.8 1.0 O2G C:GTP184 4.4 26.8 1.0 CG C:ASP63 4.4 24.3 1.0 O1A C:GTP184 4.4 28.4 1.0 CG2 C:THR27 4.4 28.1 1.0 O3A C:GTP184 4.6 26.0 1.0 C C:PRO43 4.6 33.0 1.0 PA C:GTP184 4.9 27.7 1.0 CA C:GLY65 4.9 25.9 1.0 ND2 C:ASN48 4.9 30.4 1.0 O2A C:GTP184 5.0 27.9 1.0 CA C:PRO43 5.0 34.3 1.0

Magnesium binding site 3 out of 4 in the The Crystal Structure of ARF6-MKLP1 (Mitotic Kinesin-Like Protein 1) Complex


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of The Crystal Structure of ARF6-MKLP1 (Mitotic Kinesin-Like Protein 1) Complex within 5.0Å range: probe atom residue distance (Å) B Occ E:Mg185 b:39.7 occ:1.00 O E:HOH204 1.7 37.9 1.0 O1G E:GTP184 1.9 35.4 1.0 O1B E:GTP184 2.0 36.2 1.0 O E:HOH201 2.2 30.4 1.0 OG1 E:THR27 2.3 33.9 1.0 OG1 E:THR44 2.4 36.9 1.0 PG E:GTP184 3.1 36.0 1.0 CB E:THR44 3.1 36.1 1.0 PB E:GTP184 3.2 36.8 1.0 CB E:THR27 3.5 35.0 1.0 O3B E:GTP184 3.5 37.8 1.0 O3G E:GTP184 3.5 35.0 1.0 N E:THR44 3.8 40.0 1.0 OD2 E:ASP63 3.8 32.8 1.0 OD1 E:ASP63 3.9 33.0 1.0 O2B E:GTP184 4.0 35.0 1.0 N E:THR27 4.0 33.8 1.0 CA E:THR44 4.0 38.3 1.0 CG E:ASP63 4.3 31.9 1.0 CG2 E:THR44 4.3 35.0 1.0 CA E:THR27 4.3 34.3 1.0 O2G E:GTP184 4.4 37.6 1.0 O1A E:GTP184 4.4 36.2 1.0 O3A E:GTP184 4.5 36.6 1.0 CG2 E:THR27 4.5 35.7 1.0 O E:ILE42 4.6 45.7 1.0 CA E:GLY65 4.8 32.6 1.0 PA E:GTP184 4.8 36.4 1.0 C E:PRO43 4.8 43.1 1.0 CB E:LYS26 4.8 32.0 1.0 CE E:LYS26 5.0 27.4 1.0 O2A E:GTP184 5.0 34.9 1.0

Magnesium binding site 4 out of 4 in the The Crystal Structure of ARF6-MKLP1 (Mitotic Kinesin-Like Protein 1) Complex


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 4 of The Crystal Structure of ARF6-MKLP1 (Mitotic Kinesin-Like Protein 1) Complex within 5.0Å range: probe atom residue distance (Å) B Occ G:Mg185 b:56.9 occ:1.00 O1G G:GTP184 1.9 60.3 1.0 OG1 G:THR27 2.1 64.5 1.0 O1B G:GTP184 2.2 61.5 1.0 OG1 G:THR44 2.3 59.1 1.0 CB G:THR44 3.0 57.6 1.0 PG G:GTP184 3.1 60.2 1.0 PB G:GTP184 3.4 61.3 1.0 CB G:THR27 3.5 66.8 1.0 O3B G:GTP184 3.6 61.4 1.0 OD2 G:ASP63 3.7 59.4 1.0 O3G G:GTP184 3.7 57.2 1.0 OD1 G:ASP63 3.8 55.9 1.0 N G:THR44 3.9 60.7 1.0 CA G:THR44 4.0 59.4 1.0 N G:THR27 4.1 63.9 1.0 O2B G:GTP184 4.1 59.4 1.0 CG2 G:THR44 4.1 57.2 1.0 CG G:ASP63 4.2 57.6 1.0 O2G G:GTP184 4.3 62.7 1.0 CA G:THR27 4.3 66.1 1.0 CG2 G:THR27 4.4 68.8 1.0 O1A G:GTP184 4.5 66.6 1.0 O3A G:GTP184 4.7 63.0 1.0 O G:ILE42 4.8 67.8 1.0 O2A G:GTP184 4.8 64.5 1.0 CE G:LYS26 4.9 52.7 1.0 CB G:LYS26 4.9 58.9 1.0 PA G:GTP184 4.9 65.1 1.0 CA G:GLY65 4.9 55.6 1.0

H.Makyio, M.Ohgi, T.Takei, S.Takahashi, H.Takatsu, Y.Katoh, A.Hanai, T.Ueda, Y.Kanaho, Y.Xie, H.W.Shin, H.Kamikubo, M.Kataoka, M.Kawasaki, R.Kato, S.Wakatsuki, K.Nakayama. Structural Basis For ARF6-MKLP1 Complex Formation on the Flemming Body Responsible For Cytokinesis Embo J. V. 31 2590 2012.
ISSN: ISSN 0261-4189
PubMed: 22522702
DOI: 10.1038/EMBOJ.2012.89