Magnesium in PDB 3x2v: Michaelis-Like Complex of Camp-Dependent Protein Kinase Catalytic Subunit

All present enzymatic activity of Michaelis-Like Complex of Camp-Dependent Protein Kinase Catalytic Subunit:
2.7.11.11;

The structure of Michaelis-Like Complex of Camp-Dependent Protein Kinase Catalytic Subunit, PDB code: 3x2v was solved by A.Das, P.Langan, O.Gerlits, A.Y.Kovalevsky, W.T.Heller, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	35.17 / 1.77
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	61.310, 78.420, 79.580, 90.00, 90.00, 90.00
R / Rfree (%)	18.3 / 23

The binding sites of Magnesium atom in the Michaelis-Like Complex of Camp-Dependent Protein Kinase Catalytic Subunit (pdb code 3x2v). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Michaelis-Like Complex of Camp-Dependent Protein Kinase Catalytic Subunit, PDB code: 3x2v:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Michaelis-Like Complex of Camp-Dependent Protein Kinase Catalytic Subunit


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Michaelis-Like Complex of Camp-Dependent Protein Kinase Catalytic Subunit within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg401 b:6.7 occ:1.00 O3G A:ATP403 2.0 4.2 1.0 O A:HOH730 2.0 6.0 1.0 O1B A:ATP403 2.0 5.0 1.0 O A:HOH678 2.1 5.8 1.0 OD2 A:ASP184 2.2 5.4 1.0 OD1 A:ASP184 2.2 5.6 1.0 CG A:ASP184 2.5 7.6 1.0 PG A:ATP403 3.1 6.4 1.0 PB A:ATP403 3.2 7.6 1.0 O3B A:ATP403 3.4 10.3 1.0 O2G A:ATP403 3.7 6.6 1.0 MG A:MG402 3.8 4.8 1.0 CB A:ASP184 4.0 2.4 1.0 OD2 A:ASP166 4.1 6.0 1.0 O A:HOH829 4.1 11.5 0.9 OG S:SER621 4.1 7.8 1.0 O S:HOH730 4.2 11.1 0.7 O2B A:ATP403 4.3 8.7 1.0 O3A A:ATP403 4.3 7.0 1.0 NZ A:LYS72 4.3 6.6 1.0 CA A:GLY186 4.3 4.4 1.0 O1G A:ATP403 4.4 7.6 1.0 O2A A:ATP403 4.5 5.6 1.0 N A:GLY186 4.6 5.6 1.0 CB S:SER621 4.6 9.2 1.0 PA A:ATP403 4.7 4.8 1.0 O1A A:ATP403 4.7 6.4 1.0 O A:HOH887 4.8 31.6 1.0 CA A:ASP184 4.9 4.1 1.0 ND2 A:ASN171 5.0 4.5 1.0

Magnesium binding site 2 out of 2 in the Michaelis-Like Complex of Camp-Dependent Protein Kinase Catalytic Subunit


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Michaelis-Like Complex of Camp-Dependent Protein Kinase Catalytic Subunit within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg402 b:4.8 occ:1.00 OD1 A:ASN171 2.0 4.7 1.0 O2G A:ATP403 2.0 6.6 1.0 O2A A:ATP403 2.0 5.6 1.0 O A:HOH504 2.1 5.2 1.0 OD2 A:ASP184 2.1 5.4 1.0 O3B A:ATP403 2.8 10.3 1.0 PG A:ATP403 2.9 6.4 1.0 CG A:ASN171 3.0 4.8 1.0 CG A:ASP184 3.1 7.6 1.0 PA A:ATP403 3.3 4.8 1.0 ND2 A:ASN171 3.5 4.5 1.0 CB A:ASP184 3.5 2.4 1.0 O3G A:ATP403 3.6 4.2 1.0 PB A:ATP403 3.7 7.6 1.0 O3A A:ATP403 3.8 7.0 1.0 MG A:MG401 3.8 6.7 1.0 O1B A:ATP403 3.8 5.0 1.0 CE A:LYS168 4.0 3.8 1.0 OD1 A:ASP184 4.2 5.6 1.0 O1G A:ATP403 4.2 7.6 1.0 O1A A:ATP403 4.2 6.4 1.0 NZ A:LYS168 4.3 3.6 1.0 O3' A:ATP403 4.3 5.2 1.0 CB A:ASN171 4.4 4.5 1.0 O S:HOH703 4.4 7.5 1.0 O5' A:ATP403 4.4 7.2 1.0 OD2 A:ASP166 4.4 6.0 1.0 O A:HOH728 4.5 9.0 0.9 C5' A:ATP403 4.6 4.5 1.0 O A:HOH678 4.7 5.8 1.0 CA A:ASN171 4.7 3.4 1.0 O A:GLU170 4.8 6.4 1.0 C3' A:ATP403 4.8 3.1 1.0 CA A:ASP184 5.0 4.1 1.0

A.Das, O.Gerlits, J.M.Parks, P.Langan, A.Kovalevsky, W.T.Heller. Protein Kinase A Catalytic Subunit Primed For Action: Time-Lapse Crystallography of Michaelis Complex Formation. Structure V. 23 2331 2015.
ISSN: ISSN 0969-2126
PubMed: 26585512
DOI: 10.1016/J.STR.2015.10.005