Magnesium in PDB 4a35: Crystal Structure of Human Mitochondrial Enolase Superfamily Member 1 (ENOSF1)

The structure of Crystal Structure of Human Mitochondrial Enolase Superfamily Member 1 (ENOSF1), PDB code: 4a35 was solved by J.R.C.Muniz, D.S.Froese, T.Krojer, M.Vollmar, P.Canning, F.Von Delft, C.H.Arrowsmith, A.M.Edwards, J.Weigelt, C.Bountra, U.Oppermann, W.W.Yue, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.99 / 1.74
Space group	P 65 2 2
Cell size a, b, c (Å), α, β, γ (°)	84.770, 84.770, 316.300, 90.00, 90.00, 120.00
R / Rfree (%)	15.2 / 17.6

The binding sites of Magnesium atom in the Crystal Structure of Human Mitochondrial Enolase Superfamily Member 1 (ENOSF1) (pdb code 4a35). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of Human Mitochondrial Enolase Superfamily Member 1 (ENOSF1), PDB code: 4a35:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of Human Mitochondrial Enolase Superfamily Member 1 (ENOSF1)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of Human Mitochondrial Enolase Superfamily Member 1 (ENOSF1) within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1445 b:23.2 occ:1.00 O A:HOH2418 1.9 22.5 1.0 O A:HOH2387 2.0 29.2 1.0 OE1 A:GLU309 2.1 27.6 1.0 OD2 A:ASP254 2.2 20.7 1.0 OE2 A:GLU280 2.2 22.7 1.0 O A:HOH2389 2.4 39.2 1.0 CD A:GLU309 3.0 31.9 1.0 CG A:ASP254 3.1 18.6 1.0 CD A:GLU280 3.1 24.2 1.0 OD1 A:ASP254 3.4 17.3 1.0 OE2 A:GLU309 3.5 29.2 1.0 NZ A:LYS224 3.8 19.9 1.0 NZ A:LYS226 3.8 29.5 1.0 CG A:GLU280 3.9 20.1 1.0 O A:HOH2388 3.9 34.2 1.0 OE1 A:GLU280 4.0 21.6 1.0 O A:HOH2345 4.0 19.3 1.0 ND2 A:ASN256 4.0 27.0 1.0 O A:HOH2450 4.3 19.7 1.0 CG A:GLU309 4.3 24.6 1.0 CD2 A:HIS359 4.3 21.4 1.0 OE1 A:GLU281 4.4 22.4 1.0 CB A:ASP254 4.4 20.1 1.0 CE A:LYS224 4.5 25.0 1.0 NE2 A:HIS359 4.8 20.2 1.0 O A:HOH2505 4.8 47.5 1.0 CG A:ASN256 4.8 25.0 1.0 CB A:GLU309 4.9 20.5 1.0 OD1 A:ASN256 4.9 20.1 1.0 CB A:GLU280 5.0 18.1 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of Human Mitochondrial Enolase Superfamily Member 1 (ENOSF1)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of Human Mitochondrial Enolase Superfamily Member 1 (ENOSF1) within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1446 b:30.3 occ:1.00 OH A:TYR191 2.7 17.6 1.0 OD2 A:ASP376 2.9 22.6 1.0 O A:SER383 3.0 18.3 1.0 OG A:SER381 3.4 22.4 1.0 CE2 A:TYR191 3.4 18.9 1.0 CZ A:TYR191 3.5 20.9 1.0 N A:TYR377 3.6 17.3 1.0 CA A:TYR377 3.6 17.2 1.0 CB A:TYR377 3.6 17.5 1.0 CD A:ARG387 3.6 20.2 1.0 CG A:ASP376 4.0 21.2 1.0 O A:ILE373 4.0 19.1 1.0 CD2 A:LEU384 4.2 15.5 1.0 C A:SER383 4.2 20.9 1.0 C A:ASP376 4.2 20.2 1.0 CD1 A:TYR377 4.3 18.9 1.0 CB A:ASP376 4.3 17.1 1.0 CD1 A:ILE373 4.3 17.2 1.0 CG1 A:ILE373 4.3 22.5 1.0 O A:HOH2504 4.4 21.0 1.0 NE A:ARG387 4.4 18.2 1.0 CG A:TYR377 4.4 17.7 1.0 CB A:ARG387 4.4 17.4 1.0 CG A:ARG387 4.6 18.2 1.0 CB A:SER381 4.6 20.4 1.0 CD2 A:TYR191 4.7 18.4 1.0 O A:ASP376 4.7 18.9 1.0 CE1 A:TYR191 4.9 19.3 1.0 CA A:ASP376 4.9 17.0 1.0 N A:SER383 4.9 18.4 1.0 CA A:LEU384 5.0 20.1 1.0

D.J.Wichelecki, D.S.Froese, J.Kopec, J.R.Muniz, W.W.Yue, J.A.Gerlt. Enzymatic and Structural Characterization of Rts Gamma Provides Insights Into the Function of Rts Beta. Biochemistry V. 53 2732 2014.
ISSN: ISSN 1520-4995
PubMed: 24697329
DOI: 10.1021/BI500349E