Magnesium in PDB 4as8: X-Ray Structure of the Cyan Fluorescent Protein Cerulean Cryoprotected with Ethylene Glycol

The structure of X-Ray Structure of the Cyan Fluorescent Protein Cerulean Cryoprotected with Ethylene Glycol, PDB code: 4as8 was solved by D.Von Stetten, G.Batot, M.Noirclerc-Savoye, A.Royant, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	46.75 / 1.02
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	51.344, 62.662, 70.205, 90.00, 90.00, 90.00
R / Rfree (%)	11.6 / 13.2

The binding sites of Magnesium atom in the X-Ray Structure of the Cyan Fluorescent Protein Cerulean Cryoprotected with Ethylene Glycol (pdb code 4as8). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the X-Ray Structure of the Cyan Fluorescent Protein Cerulean Cryoprotected with Ethylene Glycol, PDB code: 4as8:

Magnesium binding site 1 out of 1 in the X-Ray Structure of the Cyan Fluorescent Protein Cerulean Cryoprotected with Ethylene Glycol


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of X-Ray Structure of the Cyan Fluorescent Protein Cerulean Cryoprotected with Ethylene Glycol within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg3001 b:13.7 occ:0.50 O A:HOH2496 2.0 26.1 1.0 O A:HOH2495 2.0 15.9 0.5 O A:HOH2160 2.0 22.2 1.0 O A:HOH2495 2.1 20.3 0.5 O A:HOH2494 2.1 22.8 1.0 O A:HOH2164 2.1 17.6 1.0 OD1 A:ASP129 4.2 19.5 1.0 OD2 A:ASP129 4.3 18.8 1.0 O A:HOH2072 4.4 17.9 1.0 CG A:ASP129 4.7 14.1 1.0

D.Von Stetten, G.Batot, M.Noirclerc-Savoye, A.Royant. Alteration of Fluorescent Protein Spectroscopic Properties Upon Cryoprotection Acta Crystallogr.,Sect.D V. 68 1578 2012.
ISSN: ISSN 0907-4449
PubMed: 23090407
DOI: 10.1107/S0907444912037900