Magnesium in PDB 4bbs: Structure of An Initially Transcribing Rna Polymerase II- Tfiib Complex

All present enzymatic activity of Structure of An Initially Transcribing Rna Polymerase II- Tfiib Complex:
2.7.7.6;

The structure of Structure of An Initially Transcribing Rna Polymerase II- Tfiib Complex, PDB code: 4bbs was solved by S.Sainsbury, J.Niesser, P.Cramer, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	49.273 / 3.60
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	222.170, 386.010, 254.470, 90.00, 90.00, 90.00
R / Rfree (%)	18.47 / 22.5

The structure of Structure of An Initially Transcribing Rna Polymerase II- Tfiib Complex also contains other interesting chemical elements:

Zinc

(Zn)

9 atoms

The binding sites of Magnesium atom in the Structure of An Initially Transcribing Rna Polymerase II- Tfiib Complex (pdb code 4bbs). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Structure of An Initially Transcribing Rna Polymerase II- Tfiib Complex, PDB code: 4bbs:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Structure of An Initially Transcribing Rna Polymerase II- Tfiib Complex


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Structure of An Initially Transcribing Rna Polymerase II- Tfiib Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg2458 b:0.8 occ:1.00 OD2 A:ASP481 2.1 0.1 1.0 O2' P:A10 2.9 0.3 1.0 O3' P:A10 3.0 1.0 1.0 CG A:ASP481 3.2 0.1 1.0 CB A:ASP481 3.6 0.6 1.0 OD2 A:ASP485 3.7 0.5 1.0 C2' P:A10 3.9 0.1 1.0 OD1 A:ASP483 3.9 0.2 1.0 C3' P:A10 3.9 0.1 1.0 N A:ASP481 3.9 0.0 1.0 OD1 A:ASP485 3.9 0.0 1.0 NH2 A:ARG446 4.1 88.5 1.0 OD2 A:ASP483 4.2 0.9 1.0 CG A:ASP485 4.2 0.1 1.0 OD1 A:ASP481 4.3 0.7 1.0 CA A:ASP481 4.4 0.6 1.0 CG A:ASP483 4.5 1.0 1.0 O A:ASN479 4.5 87.5 1.0 C4' P:A10 4.5 0.6 1.0 O A:ASP481 4.8 95.3 1.0 C A:ALA480 4.9 89.4 1.0 C A:ASP481 5.0 93.1 1.0

Magnesium binding site 2 out of 2 in the Structure of An Initially Transcribing Rna Polymerase II- Tfiib Complex


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Structure of An Initially Transcribing Rna Polymerase II- Tfiib Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg2459 b:0.2 occ:1.00 OD1 A:ASP481 2.1 0.7 1.0 CG A:ASP481 3.1 0.1 1.0 OD2 A:ASP481 3.5 0.1 1.0 OE1 B:GLU836 3.9 0.5 1.0 O A:ALA480 4.2 85.5 1.0 ND2 A:ASN479 4.2 0.2 1.0 CB A:ASP481 4.4 0.6 1.0 OE2 B:GLU836 4.4 0.8 1.0 C A:ALA480 4.4 89.4 1.0 N A:ASP481 4.6 0.0 1.0 CD B:GLU836 4.6 0.2 1.0 CA A:ASP481 4.7 0.6 1.0 C A:ASN479 4.8 87.3 1.0 CG2 A:THR475 4.8 93.2 1.0 O A:ASN479 4.8 87.5 1.0 N A:ALA480 4.9 86.0 1.0

S.Sainsbury, J.Niesser, P.Cramer. Structure and Function of the Initially Transcribing Rna Polymerase II-Tfiib Complex Nature V. 493 437 2013.
ISSN: ISSN 0028-0836
PubMed: 23151482
DOI: 10.1038/NATURE11715