Magnesium in PDB 4bn2: The Crystal Structure of Kinesin-Like Protein KIF15

The structure of The Crystal Structure of Kinesin-Like Protein KIF15, PDB code: 4bn2 was solved by M.Klejnot, A.Falnikar, V.Ulaganathan, R.Cross, P.Baas, F.Kozielski, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	29.486 / 2.70
Space group	P 32 2 1
Cell size a, b, c (Å), α, β, γ (°)	90.080, 90.080, 249.010, 90.00, 90.00, 120.00
R / Rfree (%)	20.18 / 25.91

The binding sites of Magnesium atom in the The Crystal Structure of Kinesin-Like Protein KIF15 (pdb code 4bn2). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 3 binding sites of Magnesium where determined in the The Crystal Structure of Kinesin-Like Protein KIF15, PDB code: 4bn2:
Jump to Magnesium binding site number: 1; 2; 3;

Magnesium binding site 1 out of 3 in the The Crystal Structure of Kinesin-Like Protein KIF15


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of The Crystal Structure of Kinesin-Like Protein KIF15 within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg601 b:15.8 occ:1.00 OG1 A:THR102 2.4 22.9 1.0 O2B A:ADP600 2.4 15.3 1.0 O A:HOH2037 2.5 18.3 1.0 O A:HOH2038 2.7 27.8 1.0 O A:HOH2036 2.9 34.9 1.0 O1B A:ADP600 3.1 24.5 1.0 PB A:ADP600 3.2 16.6 1.0 CB A:THR102 3.6 19.8 1.0 O A:HOH2035 4.2 15.9 1.0 N A:THR102 4.3 14.3 1.0 OD1 A:ASP251 4.3 30.1 1.0 O3B A:ADP600 4.4 22.1 1.0 OD2 A:ASP251 4.4 18.6 1.0 O3A A:ADP600 4.4 29.1 1.0 CA A:THR102 4.5 15.0 1.0 O1A A:ADP600 4.5 23.0 1.0 CE A:LYS101 4.6 16.3 1.0 CG2 A:THR102 4.6 7.2 1.0 NZ A:LYS101 4.7 23.2 1.0 O A:HOH2066 4.8 20.8 1.0 CG A:ASP251 4.8 27.3 1.0 PA A:ADP600 4.8 20.3 1.0 CB A:LYS101 4.9 17.2 1.0 NH2 A:ARG208 5.0 44.2 1.0 O A:SER219 5.0 34.4 1.0 O2A A:ADP600 5.0 18.0 1.0

Magnesium binding site 2 out of 3 in the The Crystal Structure of Kinesin-Like Protein KIF15


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of The Crystal Structure of Kinesin-Like Protein KIF15 within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg601 b:24.8 occ:1.00 O B:HOH2043 2.2 4.3 1.0 O B:HOH2044 2.5 9.7 1.0 O B:HOH2042 2.8 14.1 1.0 O1B B:ADP600 2.8 9.4 1.0 O2B B:ADP600 2.8 13.6 1.0 O B:HOH2041 3.2 15.5 1.0 O B:HOH2070 3.2 16.7 1.0 PB B:ADP600 3.3 16.1 1.0 OG1 B:THR102 3.7 18.6 1.0 O B:HOH2080 3.9 18.2 1.0 O3B B:ADP600 4.4 17.5 1.0 O1A B:ADP600 4.4 22.7 1.0 O3A B:ADP600 4.5 25.2 1.0 CA B:SER220 4.6 31.4 1.0 NZ B:LYS101 4.6 16.5 1.0 O B:SER219 4.7 31.0 1.0 CE B:LYS101 4.7 17.2 1.0 CG2 B:THR97 4.8 11.0 1.0 CB B:THR102 4.8 15.6 1.0 N B:SER220 5.0 44.3 1.0 C B:SER219 5.0 51.1 1.0

Magnesium binding site 3 out of 3 in the The Crystal Structure of Kinesin-Like Protein KIF15


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of The Crystal Structure of Kinesin-Like Protein KIF15 within 5.0Å range: probe atom residue distance (Å) B Occ C:Mg601 b:17.0 occ:1.00 O C:HOH2025 2.2 26.0 1.0 O C:HOH2026 2.2 20.1 1.0 O2B C:ADP600 2.3 13.7 1.0 OG1 C:THR102 2.3 24.8 1.0 O C:HOH2054 2.3 23.6 1.0 O C:HOH2027 2.6 17.8 1.0 O1B C:ADP600 3.0 16.9 1.0 PB C:ADP600 3.1 18.6 1.0 O C:HOH2042 3.4 31.3 1.0 CB C:THR102 3.5 15.8 1.0 N C:THR102 4.0 16.1 1.0 O C:HOH2024 4.1 25.6 1.0 O3B C:ADP600 4.2 21.2 1.0 OD1 C:ASP251 4.2 29.6 1.0 OD2 C:ASP251 4.2 19.6 1.0 CA C:THR102 4.3 14.1 1.0 O3A C:ADP600 4.3 27.2 1.0 O1A C:ADP600 4.3 25.9 1.0 CE C:LYS101 4.4 17.6 1.0 CG2 C:THR102 4.5 9.4 1.0 CB C:LYS101 4.6 18.8 1.0 CG C:ASP251 4.6 29.1 1.0 PA C:ADP600 4.6 24.2 1.0 NZ C:LYS101 4.7 20.0 1.0 O2A C:ADP600 4.8 19.8 1.0 O C:HOH2053 4.9 28.7 1.0 C C:LYS101 5.0 16.1 1.0

M.Klejnot, A.Falnikar, V.Ulaganathan, R.A.Cross, P.W.Baas, F.Kozielski. The Crystal Structure and Biochemical Characterization of KIF15: A Bifunctional Molecular Motor Involved in Bipolar Spindle Formation and Neuronal Development Acta Crystallogr.,Sect.D V. 70 123 2014.
ISSN: ISSN 0907-4449
PubMed: 24419385
DOI: 10.1107/S1399004713028721