Magnesium in PDB 4byf: Crystal Structure of Human Myosin 1C in Complex with Calmodulin in the Pre-Power Stroke State
Enzymatic activity of Crystal Structure of Human Myosin 1C in Complex with Calmodulin in the Pre-Power Stroke State
All present enzymatic activity of Crystal Structure of Human Myosin 1C in Complex with Calmodulin in the Pre-Power Stroke State:
3.6.4.1;
Protein crystallography data
The structure of Crystal Structure of Human Myosin 1C in Complex with Calmodulin in the Pre-Power Stroke State, PDB code: 4byf
was solved by
S.Munnich,
M.H.Taft,
S.Pathan-Chhatbar,
D.J.Manstein,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
47.94 /
2.74
|
Space group
|
P 1 21 1
|
Cell size a, b, c (Å), α, β, γ (°)
|
59.580,
158.450,
114.340,
90.00,
91.56,
90.00
|
R / Rfree (%)
|
18.3 /
23.67
|
Other elements in 4byf:
The structure of Crystal Structure of Human Myosin 1C in Complex with Calmodulin in the Pre-Power Stroke State also contains other interesting chemical elements:
Magnesium Binding Sites:
The binding sites of Magnesium atom in the Crystal Structure of Human Myosin 1C in Complex with Calmodulin in the Pre-Power Stroke State
(pdb code 4byf). This binding sites where shown within
5.0 Angstroms radius around Magnesium atom.
In total 3 binding sites of Magnesium where determined in the
Crystal Structure of Human Myosin 1C in Complex with Calmodulin in the Pre-Power Stroke State, PDB code: 4byf:
Jump to Magnesium binding site number:
1;
2;
3;
Magnesium binding site 1 out
of 3 in 4byf
Go back to
Magnesium Binding Sites List in 4byf
Magnesium binding site 1 out
of 3 in the Crystal Structure of Human Myosin 1C in Complex with Calmodulin in the Pre-Power Stroke State
 Mono view
 Stereo pair view
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 1 of Crystal Structure of Human Myosin 1C in Complex with Calmodulin in the Pre-Power Stroke State within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Mg1000
b:17.9
occ:1.00
|
H2G1
|
A:AOV1001
|
1.4
|
14.8
|
1.0
|
OG1
|
A:THR112
|
1.7
|
22.1
|
1.0
|
O2G
|
A:AOV1001
|
2.0
|
12.3
|
1.0
|
O
|
A:HOH2010
|
2.1
|
11.0
|
1.0
|
O1B
|
A:AOV1001
|
2.2
|
32.7
|
1.0
|
OG
|
A:SER161
|
2.5
|
22.0
|
1.0
|
CB
|
A:THR112
|
2.7
|
30.1
|
1.0
|
CB
|
A:SER161
|
3.4
|
30.7
|
1.0
|
PB
|
A:AOV1001
|
3.5
|
22.2
|
1.0
|
O2A
|
A:AOV1001
|
3.5
|
12.9
|
1.0
|
CA
|
A:THR112
|
3.7
|
32.2
|
1.0
|
N
|
A:THR112
|
3.8
|
32.9
|
1.0
|
CG2
|
A:THR112
|
3.8
|
22.6
|
1.0
|
VG
|
A:AOV1001
|
3.8
|
16.3
|
1.0
|
O3B
|
A:AOV1001
|
3.9
|
38.1
|
1.0
|
N
|
A:SER161
|
4.0
|
40.7
|
1.0
|
OD1
|
A:ASP386
|
4.0
|
79.9
|
1.0
|
CA
|
A:SER161
|
4.3
|
33.1
|
1.0
|
PA
|
A:AOV1001
|
4.3
|
19.9
|
1.0
|
OD2
|
A:ASP386
|
4.3
|
73.0
|
1.0
|
O
|
A:HOH2015
|
4.4
|
51.1
|
1.0
|
O1A
|
A:AOV1001
|
4.4
|
27.0
|
1.0
|
O3A
|
A:AOV1001
|
4.4
|
27.6
|
1.0
|
O2B
|
A:AOV1001
|
4.6
|
72.2
|
1.0
|
CG
|
A:ASP386
|
4.6
|
68.3
|
1.0
|
O4G
|
A:AOV1001
|
4.8
|
35.0
|
1.0
|
O1G
|
A:AOV1001
|
4.8
|
24.9
|
1.0
|
ND2
|
A:ASN151
|
4.9
|
25.8
|
1.0
|
C
|
A:LYS111
|
4.9
|
33.5
|
1.0
|
O
|
A:ASN159
|
5.0
|
46.8
|
1.0
|
C
|
A:SER160
|
5.0
|
40.1
|
1.0
|
ND2
|
A:ASN157
|
5.0
|
27.8
|
1.0
|
O
|
A:ILE387
|
5.0
|
48.5
|
1.0
|
|
Magnesium binding site 2 out
of 3 in 4byf
Go back to
Magnesium Binding Sites List in 4byf
Magnesium binding site 2 out
of 3 in the Crystal Structure of Human Myosin 1C in Complex with Calmodulin in the Pre-Power Stroke State
 Mono view
 Stereo pair view
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 2 of Crystal Structure of Human Myosin 1C in Complex with Calmodulin in the Pre-Power Stroke State within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
C:Mg1000
b:23.6
occ:1.00
|
O2G
|
C:AOV1001
|
1.6
|
40.6
|
1.0
|
O
|
C:HOH2021
|
1.7
|
23.7
|
1.0
|
O
|
C:HOH2019
|
1.9
|
16.4
|
1.0
|
OG1
|
C:THR112
|
2.1
|
26.5
|
1.0
|
O1B
|
C:AOV1001
|
2.2
|
21.7
|
1.0
|
H2G1
|
C:AOV1001
|
2.3
|
48.7
|
1.0
|
OG
|
C:SER161
|
2.6
|
33.6
|
1.0
|
CB
|
C:THR112
|
3.2
|
42.8
|
1.0
|
CB
|
C:SER161
|
3.2
|
38.5
|
1.0
|
VG
|
C:AOV1001
|
3.3
|
35.6
|
1.0
|
PB
|
C:AOV1001
|
3.4
|
27.0
|
1.0
|
O3B
|
C:AOV1001
|
3.5
|
46.0
|
1.0
|
O2A
|
C:AOV1001
|
3.8
|
55.5
|
1.0
|
N
|
C:SER161
|
3.9
|
45.5
|
1.0
|
OD1
|
C:ASP386
|
3.9
|
83.8
|
1.0
|
O3G
|
C:AOV1001
|
4.1
|
48.6
|
1.0
|
N
|
C:THR112
|
4.1
|
44.9
|
1.0
|
O4G
|
C:AOV1001
|
4.1
|
56.6
|
1.0
|
CA
|
C:THR112
|
4.2
|
37.6
|
1.0
|
CA
|
C:SER161
|
4.2
|
41.5
|
1.0
|
CG2
|
C:THR112
|
4.2
|
45.2
|
1.0
|
O2B
|
C:AOV1001
|
4.3
|
44.8
|
1.0
|
OD2
|
C:ASP386
|
4.4
|
79.0
|
1.0
|
O
|
C:HOH2020
|
4.5
|
36.6
|
1.0
|
O
|
C:ILE387
|
4.5
|
43.3
|
1.0
|
O3A
|
C:AOV1001
|
4.5
|
12.4
|
1.0
|
CG
|
C:ASP386
|
4.6
|
77.2
|
1.0
|
PA
|
C:AOV1001
|
4.7
|
40.8
|
1.0
|
ND2
|
C:ASN157
|
5.0
|
40.6
|
1.0
|
C
|
C:SER160
|
5.0
|
46.3
|
1.0
|
|
Magnesium binding site 3 out
of 3 in 4byf
Go back to
Magnesium Binding Sites List in 4byf
Magnesium binding site 3 out
of 3 in the Crystal Structure of Human Myosin 1C in Complex with Calmodulin in the Pre-Power Stroke State
 Mono view
 Stereo pair view
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 3 of Crystal Structure of Human Myosin 1C in Complex with Calmodulin in the Pre-Power Stroke State within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
D:Mg1000
b:48.3
occ:1.00
|
OD1
|
D:ASP94
|
2.2
|
54.3
|
1.0
|
O
|
D:TYR100
|
2.4
|
33.6
|
1.0
|
OD1
|
D:ASN98
|
2.4
|
54.2
|
1.0
|
OD2
|
D:ASP96
|
2.9
|
77.5
|
1.0
|
CG
|
D:ASP94
|
3.2
|
42.6
|
1.0
|
O
|
D:HOH2010
|
3.3
|
38.0
|
1.0
|
CG
|
D:ASP96
|
3.3
|
76.3
|
1.0
|
CB
|
D:ASP96
|
3.3
|
62.4
|
1.0
|
C
|
D:TYR100
|
3.5
|
31.2
|
1.0
|
CG
|
D:ASN98
|
3.5
|
55.2
|
1.0
|
N
|
D:ASP96
|
3.6
|
51.8
|
1.0
|
OD2
|
D:ASP94
|
3.9
|
33.7
|
1.0
|
CA
|
D:ASP96
|
4.0
|
50.1
|
1.0
|
N
|
D:TYR100
|
4.1
|
35.9
|
1.0
|
N
|
D:LYS95
|
4.1
|
42.8
|
1.0
|
ND2
|
D:ASN98
|
4.1
|
60.4
|
1.0
|
CB
|
D:ASP94
|
4.2
|
31.2
|
1.0
|
CA
|
D:TYR100
|
4.2
|
31.6
|
1.0
|
N
|
D:GLY97
|
4.3
|
36.5
|
1.0
|
OD1
|
D:ASP96
|
4.3
|
85.9
|
1.0
|
CA
|
D:ASP94
|
4.3
|
30.8
|
1.0
|
C
|
D:ASP94
|
4.4
|
36.7
|
1.0
|
N
|
D:ASN98
|
4.4
|
33.4
|
1.0
|
N
|
D:ILE101
|
4.6
|
21.6
|
1.0
|
CB
|
D:TYR100
|
4.6
|
24.0
|
1.0
|
C
|
D:LYS95
|
4.7
|
45.8
|
1.0
|
C
|
D:ASP96
|
4.7
|
43.1
|
1.0
|
CB
|
D:ASN98
|
4.7
|
43.9
|
1.0
|
CA
|
D:ILE101
|
4.8
|
30.9
|
1.0
|
N
|
D:GLY99
|
4.8
|
30.6
|
1.0
|
CA
|
D:LYS95
|
4.9
|
46.7
|
1.0
|
CG1
|
D:ILE101
|
5.0
|
39.5
|
1.0
|
CA
|
D:ASN98
|
5.0
|
37.4
|
1.0
|
|
Reference:
S.Munnich,
M.H.Taft,
D.J.Manstein.
Crystal Structure of Human Myosin 1C-the Motor in GLUT4 Exocytosis: Implications For Ca(2+) Regulation and 14-3-3 Binding. J.Mol.Biol. V. 426 2070 2014.
ISSN: ISSN 0022-2836
PubMed: 24636949
DOI: 10.1016/J.JMB.2014.03.004
Page generated: Thu Aug 15 16:37:36 2024
|