Magnesium in PDB 4ct4: CNOT1 MIF4G Domain - DDX6 Complex

The structure of CNOT1 MIF4G Domain - DDX6 Complex, PDB code: 4ct4 was solved by S.Ozgur, J.Basquin, E.Conti, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	64.290 / 2.30
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	71.531, 120.014, 78.469, 90.00, 104.01, 90.00
R / Rfree (%)	16.6 / 21.98

The structure of CNOT1 MIF4G Domain - DDX6 Complex also contains other interesting chemical elements:

Chlorine

(Cl)

1 atom

The binding sites of Magnesium atom in the CNOT1 MIF4G Domain - DDX6 Complex (pdb code 4ct4). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 4 binding sites of Magnesium where determined in the CNOT1 MIF4G Domain - DDX6 Complex, PDB code: 4ct4:
Jump to Magnesium binding site number: 1; 2; 3; 4;

Magnesium binding site 1 out of 4 in the CNOT1 MIF4G Domain - DDX6 Complex


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of CNOT1 MIF4G Domain - DDX6 Complex within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg1470 b:61.3 occ:1.00 N B:GLY201 3.5 42.0 1.0 OG1 B:THR222 3.5 35.3 1.0 NH2 B:ARG174 3.6 60.1 1.0 CA B:GLY201 3.8 44.7 1.0 NE B:ARG174 3.8 67.3 1.0 CG2 B:THR222 3.9 33.7 1.0 CB B:THR222 3.9 36.4 1.0 O B:HOH2044 4.0 35.3 1.0 MG B:MG1471 4.2 63.5 1.0 CZ B:ARG174 4.2 63.8 1.0 CB B:THR200 4.4 40.7 1.0 O B:HOH2045 4.5 39.3 1.0 CB B:ARG174 4.6 37.2 1.0 C B:GLY201 4.6 52.7 1.0 N B:GLY202 4.6 51.3 1.0 C B:THR200 4.6 42.4 1.0 N B:ARG174 4.6 38.9 1.0 OG1 B:THR200 4.9 44.6 1.0 CA B:THR200 4.9 39.5 1.0 O B:HOH2108 4.9 53.0 1.0 CG B:ARG174 5.0 66.4 1.0 CA B:ARG174 5.0 44.5 1.0 CD B:ARG174 5.0 69.1 1.0

Magnesium binding site 2 out of 4 in the CNOT1 MIF4G Domain - DDX6 Complex


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of CNOT1 MIF4G Domain - DDX6 Complex within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg1471 b:63.5 occ:1.00 N B:ARG174 3.3 38.9 1.0 MG B:MG1472 3.5 65.5 1.0 CG B:ARG174 3.5 66.4 1.0 CA B:THR173 3.5 42.7 1.0 CB B:THR173 3.7 47.5 1.0 NE B:ARG174 3.8 67.3 1.0 C B:THR173 3.9 38.8 1.0 CD B:ARG174 4.0 69.1 1.0 CG2 B:THR173 4.1 38.4 1.0 MG B:MG1470 4.2 61.3 1.0 CB B:ARG174 4.2 37.2 1.0 CA B:ARG174 4.3 44.5 1.0 O B:PRO172 4.5 43.0 1.0 CZ B:ARG174 4.7 63.8 1.0 N B:THR173 4.8 44.1 1.0 OG1 B:THR173 4.9 43.8 1.0

Magnesium binding site 3 out of 4 in the CNOT1 MIF4G Domain - DDX6 Complex


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of CNOT1 MIF4G Domain - DDX6 Complex within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg1472 b:65.5 occ:1.00 N B:GLU175 3.3 44.3 1.0 CB B:GLU175 3.4 45.6 1.0 CG B:ARG174 3.4 66.4 1.0 CB B:THR173 3.5 47.5 1.0 MG B:MG1471 3.5 63.5 1.0 N B:ARG174 3.7 38.9 1.0 ND2 B:ASN374 3.8 76.3 1.0 OG1 B:THR173 3.9 43.8 1.0 CA B:GLU175 3.9 37.0 1.0 CG2 B:THR173 4.3 38.4 1.0 C B:ARG174 4.3 46.2 1.0 C B:THR173 4.3 38.8 1.0 CA B:ARG174 4.3 44.5 1.0 CA B:THR173 4.4 42.7 1.0 CD B:ARG174 4.4 69.1 1.0 CB B:ARG174 4.4 37.2 1.0 CG B:GLU175 4.7 66.2 1.0 CG B:GLN370 4.8 67.5 1.0 CG B:ASN374 5.0 69.4 1.0

Magnesium binding site 4 out of 4 in the CNOT1 MIF4G Domain - DDX6 Complex


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 4 of CNOT1 MIF4G Domain - DDX6 Complex within 5.0Å range: probe atom residue distance (Å) B Occ D:Mg1463 b:49.1 occ:1.00 OG1 D:THR417 3.1 44.1 1.0 N D:LYS413 3.2 57.0 1.0 N D:LEU414 3.3 54.1 1.0 CG D:LEU414 3.5 51.9 1.0 CB D:THR417 3.5 39.1 1.0 CB D:LEU414 3.5 47.5 1.0 CD1 D:LEU414 3.7 53.4 1.0 CG2 D:THR417 3.8 46.6 1.0 CA D:LYS413 3.9 60.6 1.0 CB D:LYS413 4.0 63.3 1.0 CB D:PRO412 4.0 48.5 1.0 CA D:LEU414 4.0 54.2 1.0 C D:LYS413 4.0 60.7 1.0 C D:PRO412 4.1 56.3 1.0 CA D:PRO412 4.2 48.1 1.0 CG D:LYS413 4.2 74.0 1.0 O D:LEU414 4.7 49.6 1.0 C D:LEU414 4.8 49.7 1.0 CD2 D:LEU414 4.9 55.8 1.0 CA D:THR417 4.9 46.6 1.0

H.Mathys, J.Basquin, S.Ozgur, M.Czarnocki-Cieciura, F.Bonneau, A.Aartse, A.Dziembowski, M.Nowotny, E.Conti, W.Filipowicz. Structural and Biochemical Insights to the Role of the CCR4- Not Complex and DDX6 Atpase in Microrna Repression. Mol.Cell V. 54 751 2014.
ISSN: ISSN 1097-2765
PubMed: 24768538
DOI: 10.1016/J.MOLCEL.2014.03.036