Magnesium in PDB 4czy: Complex of Neurospora Crassa PAN2 (WD40-CS1) with PAN3 (Pseudokinase and C-Term)

All present enzymatic activity of Complex of Neurospora Crassa PAN2 (WD40-CS1) with PAN3 (Pseudokinase and C-Term):
3.1.13.4;

The structure of Complex of Neurospora Crassa PAN2 (WD40-CS1) with PAN3 (Pseudokinase and C-Term), PDB code: 4czy was solved by S.Jonas, E.Izaurralde, O.Weichenrieder, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	48.74 / 3.40
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	56.266, 139.976, 157.444, 90.00, 97.81, 90.00
R / Rfree (%)	22.11 / 26.29

The binding sites of Magnesium atom in the Complex of Neurospora Crassa PAN2 (WD40-CS1) with PAN3 (Pseudokinase and C-Term) (pdb code 4czy). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Complex of Neurospora Crassa PAN2 (WD40-CS1) with PAN3 (Pseudokinase and C-Term), PDB code: 4czy:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Complex of Neurospora Crassa PAN2 (WD40-CS1) with PAN3 (Pseudokinase and C-Term)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Complex of Neurospora Crassa PAN2 (WD40-CS1) with PAN3 (Pseudokinase and C-Term) within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg1651 b:0.8 occ:1.00 O2B B:ANP1650 2.1 0.5 1.0 O3G B:ANP1650 2.1 0.5 1.0 OD1 B:ASP271 2.4 90.5 1.0 O1A B:ANP1650 2.5 0.4 1.0 CG B:ASP271 3.1 0.2 1.0 OD2 B:ASP271 3.1 97.3 1.0 PB B:ANP1650 3.4 0.1 1.0 PG B:ANP1650 3.4 0.6 1.0 O B:SER285 3.6 0.2 1.0 N3B B:ANP1650 3.8 0.9 1.0 PA B:ANP1650 3.9 0.7 1.0 O3A B:ANP1650 4.0 0.6 1.0 O2G B:ANP1650 4.2 0.0 1.0 CG B:ARG276 4.3 0.5 1.0 OG B:SER285 4.4 1.0 1.0 O B:LYS273 4.4 0.2 1.0 CB B:ARG276 4.5 0.9 1.0 CB B:ASP271 4.5 0.8 1.0 CD B:ARG276 4.5 0.2 1.0 O1G B:ANP1650 4.5 0.1 1.0 O1B B:ANP1650 4.6 0.3 1.0 C B:SER285 4.7 0.5 1.0 O5' B:ANP1650 4.7 0.5 1.0 NE B:ARG276 4.9 0.1 1.0 CA B:ARG276 4.9 0.1 1.0

Magnesium binding site 2 out of 2 in the Complex of Neurospora Crassa PAN2 (WD40-CS1) with PAN3 (Pseudokinase and C-Term)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Complex of Neurospora Crassa PAN2 (WD40-CS1) with PAN3 (Pseudokinase and C-Term) within 5.0Å range: probe atom residue distance (Å) B Occ D:Mg1651 b:0.9 occ:1.00 O2B D:ANP1650 2.2 0.9 1.0 O2G D:ANP1650 2.2 0.8 1.0 OD1 D:ASP271 2.4 0.9 1.0 CG D:ASP271 3.2 0.1 1.0 OD2 D:ASP271 3.2 90.5 1.0 O1A D:ANP1650 3.3 0.8 1.0 PB D:ANP1650 3.4 0.1 1.0 PG D:ANP1650 3.5 0.4 1.0 N3B D:ANP1650 3.7 0.1 1.0 O D:SER285 3.7 0.8 1.0 NH1 D:ARG276 3.8 0.7 1.0 O3A D:ANP1650 4.1 0.2 1.0 OG D:SER285 4.2 0.1 1.0 O1G D:ANP1650 4.4 0.0 1.0 PA D:ANP1650 4.4 0.3 1.0 O3G D:ANP1650 4.5 0.0 1.0 CB D:ASP271 4.6 0.1 1.0 O1B D:ANP1650 4.7 90.5 1.0 C D:SER285 4.7 0.5 1.0 CZ D:ARG276 4.8 0.1 1.0 O D:LYS273 4.9 0.8 1.0

S.Jonas, M.Christie, D.Peter, D.Bhandari, B.Loh, E.Huntzinger, O.Weichenrieder, E.Izaurralde. An Asymmetric PAN3 Dimer Recruits A Single PAN2 Exonuclease to Mediate Mrna Deadenylation and Decay. Nat.Struct.Mol.Biol. V. 21 599 2014.
ISSN: ISSN 1545-9993
PubMed: 24880343
DOI: 10.1038/NSMB.2837